Evaluating putative chimeric sequences from PCR-amplified products.

BACKGROUND
PCR amplification of extremely homologous genes from complicated DNA mixtures is understood to generate a major proportion of chimeric sequences. Ribosomal RNA genes are used for microbial species detection and identification in pure environments, and present assessments of microbial range are based mostly on these sequences. Thus, chimeric sequences may result in the invention of non-existent microbial species and false range estimates.
METHODS
In essence, our solely supply of data to determine if a sequence is chimeric or not is to match it with identified, non-chimeric sequences. Putative chimeric sequences had been analyzed from sequence fragments of chosen size (known as phrases) by evaluating nucleotides at corresponding positions. Distances for every phrase between reference sequences (intently associated to the examined sequence) had been in comparison with the variations launched by the examined sequence. The proposed technique considers the precise variability present in several areas all through the analyzed sequences. The result’s an environment friendly technique for the analysis of putative chimeric sequences.

Localized transfection on arrays of magnetic beads coated with PCR merchandise.

Excessive-throughput gene evaluation would profit from new approaches for delivering DNA or RNA into cells. Right here we describe a easy system that enables any molecular biology laboratory to hold out a number of, parallel cell transfections on microscope coverslip arrays. By utilizing magnetically outlined positions and PCR product-coated paramagnetic beads, we achieved transfection in a wide range of cell strains. Beads could also be added to the cells at any time, permitting each spatial and temporal management of transfection. As a result of the beads could also be coated with a couple of gene assemble, the tactic can be utilized to attain cotransfection inside single cells. Moreover, PCR-generated mutants could also be conveniently screened, bypassing cloning and plasmid purification steps. We illustrated the applicability of the tactic by screening combinatorial peptide libraries, fused to GFP, to establish beforehand unknown mobile localization motifs. On this approach, we recognized a number of localizing peptides, together with structured localization alerts based mostly across the scaffold of a single C2H2 zinc finger. The thermophilic, aciduric Alicyclobacillus spp. have gotten an rising spoilage concern within the beverage business. Speedy strategies to detect their presence in each uncooked supplies and remaining merchandise are fascinating for industrial high quality management.
The target of this examine was to develop a real-time TaqMan-based polymerase chain response (PCR) system for fast and particular detection of thermophilic spoilage micro organism, primarily Alicyclobacillus spp., for meals business functions. Two primers and a fluorogenic probe concentrating on 16S rRNA encoding gene sequences, able to detecting the genus Alicyclobacillus and some different intently associated thermophiles, had been developed, and the effectivity of the detection system was evaluated in each bacterial medium and juice merchandise. Utilizing this technique, the presence of lower than 100 Alicyclobacillus cells could possibly be detected with out cross-reactivity with different frequent food-borne micro organism. Identification of 10 white fish species related to U.Okay. meals merchandise was achieved utilizing PCR-RFLP of the mitochondrial cytochrome b gene. Use of lab-on-a-chip capillary electrophoresis for end-point evaluation enabled correct sizing of DNA fragments and identification of fish species at a stage of 5% (w/w) in a fish admixture. One restriction enzyme, DdeI, allowed discrimination of eight species. When mixed with NlaIII and HaeIII, particular profiles for all 10 species had been generated.
The strategy was utilized to a spread of merchandise and subjected to an interlaboratory examine carried out by 5 U.Okay. meals management laboratories. 100% right identification of single species samples and 6 of 9 admixture samples was achieved by all laboratories. The outcomes indicated that fish species identification could possibly be carried out utilizing a database of PCR-RFLP profiles with out the necessity for reference supplies.

Direct detection of bacterial pathogens in consultant dairy merchandise utilizing a mixed bacterial concentration-PCR strategy.

OBJECTIVE
To develop a easy, fast methodology to pay attention and purify micro organism and their nucleic acids from complicated dairy meals matrices in preparation for direct pathogen detection utilizing polymerase chain response (PCR).
RESULTS
Plain non-fat yogurt and cheddar cheese had been every seeded with Listeria monocytogenes or Salmonella enterica serovar. Enteritidis within thevary of 10(1)-10(6) CFU per 11-g pattern. Samples had been then processed for bacterial focusutilizing high-speed centrifugation (9700 g) adopted by DNA extraction, PCR amplification, and amplicon affirmation by hybridization. Bacterial recoveries after centrifugation ranged from 53 to >100% and 71 to >100% for serovar. Enteritidis and L. monocytogenes, respectively, within the non-fat yogurt samples; and from 77 to >100% and 69 to >100% for serovar. Enteritidis and L. monocytogenes, respectively, within the cheddar cheese samples. There have been no important variations in restoration effectivity at totally different inocula ranges, and losses to discarded supernatants had been at all times <5%, no matter dairy product or pathogen.

Human Advanced Oxidation Protein Products ELISA Kit (AOPP)

RK00906 96 Tests
EUR 625.2

Human AOPP(Advanced Oxidation Protein Products) ELISA Kit

ELK5337-48T 48T Ask for price
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AOPP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AOPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AOPP in the samples is then determined by comparing the OD of the samples to the standard curve.

Human AOPP(Advanced Oxidation Protein Products) ELISA Kit

ELK5337-96T 96T Ask for price
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AOPP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AOPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AOPP in the samples is then determined by comparing the OD of the samples to the standard curve.

Rat Advanced oxidation protein products ELISA kit

E02A0832-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Advanced oxidation protein products ELISA kit

E02A0832-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Advanced oxidation protein products ELISA kit

E02A0832-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Advanced oxidation protein products ELISA kit

E01A9123 96T
EUR 700
Description: ELISA

Pig Advanced oxidation protein products ELISA kit

E07A0832-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Advanced oxidation protein products ELISA kit

E07A0832-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Advanced oxidation protein products ELISA kit

E07A0832-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Advanced oxidation protein products ELISA kit

E08A0832-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Advanced oxidation protein products ELISA kit

E08A0832-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Advanced oxidation protein products ELISA kit

E08A0832-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Advanced oxidation protein products ELISA kit

E01A44044 96T
EUR 700
Description: ELISA

Goat Advanced oxidation protein products ELISA kit

E06A0832-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Advanced oxidation protein products ELISA kit

E06A0832-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Advanced oxidation protein products ELISA kit

E06A0832-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Sheep Advanced oxidation protein products ELISA kit

E01A96337 96T
EUR 700
Description: ELISA

Mouse Advanced oxidation protein products ELISA kit

E03A0832-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Advanced oxidation protein products ELISA kit

E03A0832-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Advanced oxidation protein products ELISA kit

E03A0832-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Advanced oxidation protein products ELISA kit

E01A17865 96T
EUR 700
Description: ELISA

Human advanced oxidation protein products,AOPP ELISA Kit

201-12-1267 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human advanced oxidation protein products(AOPP) Elisa Kit

EK710883 96 Wells
EUR 0.12

Human advanced oxidation protein products(AOPP) Elisa Kit

EK700152 96 Wells
EUR 0.15

Human Advanced Oxidation Protein Products (AOPP) ELISA Kit

EKU08721-48T 48T
EUR 527.66

Human Advanced Oxidation Protein Products (AOPP) ELISA Kit

EKU08721-5x96T 5x96T
EUR 3580.55

Human Advanced Oxidation Protein Products (AOPP) ELISA Kit

EKU08721-96T 96T
EUR 753.8

Human Advanced Oxidation Protein Products (AOPP) ELISA Kit

EKN49832-48T 48T
EUR 328.44

Human Advanced Oxidation Protein Products (AOPP) ELISA Kit

EKN49832-5x96T 5x96T
EUR 2228.7

Human Advanced Oxidation Protein Products (AOPP) ELISA Kit

EKN49832-96T 96T
EUR 469.2

Human advanced oxidation protein products(AOPP)ELISA Kit

GA-E1283HM-48T 48T
EUR 346.8

Human advanced oxidation protein products(AOPP)ELISA Kit

GA-E1283HM-96T 96T
EUR 559.2

Human Advanced oxidation protein products (AOPP) ELISA Kit

EK12126 96Т
EUR 799

Human Advanced Oxidation Protein Products (AOPP) ELISA Kit

CEB223Hu-10x96wellstestplate 10x96-wells test plate
EUR 5402.92
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human Advanced Oxidation Protein Products (AOPP) in serum, plasma and other biological fluids.

Human Advanced Oxidation Protein Products (AOPP) ELISA Kit

CEB223Hu-1x48wellstestplate 1x48-wells test plate
EUR 550.13
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human Advanced Oxidation Protein Products (AOPP) in serum, plasma and other biological fluids.

Human Advanced Oxidation Protein Products (AOPP) ELISA Kit

CEB223Hu-1x96wellstestplate 1x96-wells test plate
EUR 734.46
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human Advanced Oxidation Protein Products (AOPP) in serum, plasma and other biological fluids.

Human Advanced Oxidation Protein Products (AOPP) ELISA Kit

CEB223Hu-5x96wellstestplate 5x96-wells test plate
EUR 2945.08
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human Advanced Oxidation Protein Products (AOPP) in serum, plasma and other biological fluids.

Human Advanced Oxidation Protein Products (AOPP) ELISA Kit

4-CEB223Hu
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  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Advanced Oxidation Protein Products (AOPP) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Advanced oxidation protein products (AOPP) ELISA Kit

AE63142HU-48Tests 48 Tests
EUR 360
Description: Human (Homo sapiens)

Human Advanced oxidation protein products (AOPP) ELISA Kit

AE63142HU-96Tests 96 Tests
EUR 680
Description: Human (Homo sapiens)

Human advanced oxidation protein products,AOPP ELISA Kit

CN-04217H1 96T
EUR 573.6

Human advanced oxidation protein products,AOPP ELISA Kit

CN-04217H2 48T
EUR 394.8

Human Advanced Oxidation Protein Products (AOPP) ELISA Kit

20-abx258311
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human advanced oxidation protein products(AOPP)ELISA Kit

QY-E01116 96T
EUR 433.2

Human Advanced oxidation protein products (AOPP) ELISA Kit

NSL2251Hu 96 wells
EUR 468

Human Advanced Oxidation Protein Products(AOPP) ELISA Kit

SL0078Hu 96 Tests
EUR 468

Human advanced oxidation protein products,AOPP ELISA Kit

YLA0085HU-48T 48T Ask for price

Human advanced oxidation protein products,AOPP ELISA Kit

YLA0085HU-96T 96T Ask for price

Human Advanced Oxidation Protein Products (AOPP) ELISA Kit

abx258311-1mg 1 mg
EUR 5962.5

Human Advanced Oxidation Protein Products (AOPP) ELISA Kit

abx258311-20g 20 µg
EUR 3150

Human Advanced Oxidation Protein Products (AOPP) ELISA Kit

abx258311-5g 5 µg
EUR 662.5

Canine Advanced oxidation protein products ELISA kit

E01A61479 96T
EUR 700
Description: ELISA

Rabbit Advanced oxidation protein products ELISA kit

E01A26612 96T
EUR 700
Description: ELISA

Rabbit Advanced oxidation protein products ELISA kit

E04A0832-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Advanced oxidation protein products ELISA kit

E04A0832-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Advanced oxidation protein products ELISA kit

E04A0832-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Bovine Advanced oxidation protein products ELISA kit

E01A78908 96T
EUR 700
Description: ELISA

Monkey Advanced oxidation protein products ELISA kit

E01A70191 96T
EUR 700
Description: ELISA

Monkey Advanced oxidation protein products ELISA kit

E09A0832-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Monkey Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Advanced oxidation protein products ELISA kit

E09A0832-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Monkey Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Advanced oxidation protein products ELISA kit

E09A0832-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Monkey Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Porcine Advanced oxidation protein products ELISA kit

E01A52761 96T
EUR 700
Description: ELISA

Chicken Advanced oxidation protein products ELISA kit

E01A87634 96T
EUR 700
Description: ELISA

Rat AOPP(Advanced Oxidation Protein Products) ELISA Kit

ELK0784-48T 48T Ask for price
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat AOPP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AOPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AOPP in the samples is then determined by comparing the OD of the samples to the standard curve.

Rat AOPP(Advanced Oxidation Protein Products) ELISA Kit

ELK0784-96T 96T Ask for price
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat AOPP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AOPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AOPP in the samples is then determined by comparing the OD of the samples to the standard curve.

ELISA kit for Human Advanced oxidation protein products (AOPP)

KTE62659-48T 48T
EUR 424.8
Description: Quantitative sandwich ELISA for measuring Human Advanced oxidation protein products (AOPP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Advanced oxidation protein products (AOPP)

KTE62659-5platesof96wells 5 plates of 96 wells
EUR 2702.4
Description: Quantitative sandwich ELISA for measuring Human Advanced oxidation protein products (AOPP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Advanced oxidation protein products (AOPP)

KTE62659-96T 96T
EUR 686.4
Description: Quantitative sandwich ELISA for measuring Human Advanced oxidation protein products (AOPP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Guinea Pig Advanced oxidation protein products ELISA kit

E01A35326 96T
EUR 700
Description: ELISA

Mouse AOPP(Advanced Oxidation Protein Products) ELISA Kit

ELK9292-48T 48T Ask for price
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse AOPP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse AOPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse AOPP in the samples is then determined by comparing the OD of the samples to the standard curve.

Mouse AOPP(Advanced Oxidation Protein Products) ELISA Kit

ELK9292-96T 96T Ask for price
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse AOPP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse AOPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse AOPP in the samples is then determined by comparing the OD of the samples to the standard curve.

Guinea pig Advanced oxidation protein products ELISA kit

E05A0832-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Advanced oxidation protein products ELISA kit

E05A0832-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Advanced oxidation protein products ELISA kit

E05A0832-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig Advanced oxidation protein products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

ELISA kit for Human AOPP (Advanced Oxidation Protein Products)

ELK5337 1 plate of 96 wells
EUR 518.4
Description: A competitive Inhibition ELISA kit for detection of Advanced Oxidation Protein Products from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Chicken AOPP(Advanced Oxidation Protein Products) ELISA Kit

ELK9203-48T 48T Ask for price
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken AOPP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken AOPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken AOPP in the samples is then determined by comparing the OD of the samples to the standard curve.

Chicken AOPP(Advanced Oxidation Protein Products) ELISA Kit

ELK9203-96T 96T Ask for price
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken AOPP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken AOPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken AOPP in the samples is then determined by comparing the OD of the samples to the standard curve.

Rat advanced oxidation protein products(AOPP) Elisa Kit

EK721337 96 Wells
EUR 0.63

Rat advanced oxidation protein products(AOPP) ELISA kit

EKC38625-48T 48T
EUR 535.99

Rat advanced oxidation protein products(AOPP) ELISA kit

EKC38625-5x96T 5x96T
EUR 3637.08

Rat advanced oxidation protein products(AOPP) ELISA kit

EKC38625-96T 96T
EUR 765.7

Rat Advanced Oxidation Protein Products (AOPP) ELISA Kit

EKU10612-48T 48T
EUR 573.02

Rat Advanced Oxidation Protein Products (AOPP) ELISA Kit

EKU10612-5x96T 5x96T
EUR 3888.35

Rat Advanced Oxidation Protein Products (AOPP) ELISA Kit

EKU10612-96T 96T
EUR 818.6

Rat Advanced Oxidation Protein Products (AOPP) ELISA Kit

EKN51723-48T 48T
EUR 356.58

Rat Advanced Oxidation Protein Products (AOPP) ELISA Kit

EKN51723-5x96T 5x96T
EUR 2419.65

Rat Advanced Oxidation Protein Products (AOPP) ELISA Kit

EKN51723-96T 96T
EUR 509.4

Rat Advanced oxidation protein products (AOPP) ELISA Kit

EK11949 96Т
EUR 799

Rat Advanced Oxidation Protein Products (AOPP) ELISA Kit

20-abx585108
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

ELISA Kit for Advanced Oxidation Protein Products (AOPP)

CEB223Hu 96Т
EUR 665

ELISA Kit for Advanced Oxidation Protein Products (AOPP)

CEB223Mu 96Т
EUR 720

for Advanced Oxidation Protein Products (AOPP)ELISA kit

CEB223Mu-10x96wellstestplate 10x96-wells test plate
EUR 5834.88
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of for Advanced Oxidation Protein Products (AOPP) in serum, plasma and other biological fluids.

for Advanced Oxidation Protein Products (AOPP)ELISA kit

CEB223Mu-1x48wellstestplate 1x48-wells test plate
EUR 585.7
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of for Advanced Oxidation Protein Products (AOPP) in serum, plasma and other biological fluids.

for Advanced Oxidation Protein Products (AOPP)ELISA kit

CEB223Mu-1x96wellstestplate 1x96-wells test plate
EUR 785.28
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of for Advanced Oxidation Protein Products (AOPP) in serum, plasma and other biological fluids.

for Advanced Oxidation Protein Products (AOPP)ELISA kit

CEB223Mu-5x96wellstestplate 5x96-wells test plate
EUR 3173.76
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of for Advanced Oxidation Protein Products (AOPP) in serum, plasma and other biological fluids.

ELISA Kit for Advanced Oxidation Protein Products (AOPP)

4-CEB223Mu
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  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Advanced Oxidation Protein Products (AOPP) in samples from Serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

ELISA Kit for Advanced Oxidation Protein Products (AOPP)

CEB223Ra 96Т
EUR 722

Rat Advanced Oxidation Protein Products (AOPP) ELISA Kit

CEB223Ra-10x96wellstestplate 10x96-wells test plate
EUR 5850.59
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat Advanced Oxidation Protein Products (AOPP) in serum, plasma and other biological fluids.

Rat Advanced Oxidation Protein Products (AOPP) ELISA Kit

CEB223Ra-1x48wellstestplate 1x48-wells test plate
EUR 586.99
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat Advanced Oxidation Protein Products (AOPP) in serum, plasma and other biological fluids.

Rat Advanced Oxidation Protein Products (AOPP) ELISA Kit

CEB223Ra-1x96wellstestplate 1x96-wells test plate
EUR 787.13
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat Advanced Oxidation Protein Products (AOPP) in serum, plasma and other biological fluids.

Rat Advanced Oxidation Protein Products (AOPP) ELISA Kit

CEB223Ra-5x96wellstestplate 5x96-wells test plate
EUR 3182.08
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat Advanced Oxidation Protein Products (AOPP) in serum, plasma and other biological fluids.

Rat Advanced Oxidation Protein Products (AOPP) ELISA Kit

4-CEB223Ra
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  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Rat Advanced Oxidation Protein Products (AOPP) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

Rat advanced oxidation protein products(AOPP) ELISA kit

CSB-EQ027429RA-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Rat advanced oxidation protein products (AOPP) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat advanced oxidation protein products(AOPP) ELISA kit

1-CSB-EQ027429RA
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  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat advanced oxidation protein products(AOPP) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Advanced oxidation protein products (AOPP) ELISA Kit

AE62828RA-48Tests 48 Tests
EUR 325
Description: Rat (Rattus norvegicus)

Rat Advanced oxidation protein products (AOPP) ELISA Kit

AE62828RA-96Tests 96 Tests
EUR 610
Description: Rat (Rattus norvegicus)

Rat Advanced oxidation protein products(AOPP) ELISA Kit

QY-E11667 96T
EUR 433.2

Rat Advanced oxidation protein products(AOPP) ELISA Kit

NSL0914r 96 Tests
EUR 498

Rat Advanced Oxidation Protein Products(AOPP) ELISA Kit

SL1129Ra -
EUR 498

Rat advanced oxidation protein products,AOPP ELISA Kit

YLA0180RA-48T 48T Ask for price

Rat advanced oxidation protein products,AOPP ELISA Kit

YLA0180RA-96T 96T Ask for price

Goat Advanced oxidation protein products(AOPP) ELISA Kit

NSL1336Gt 96T
EUR 528

Mouse Advanced Oxidation Protein Products (AOPP) ELISA Kit

EKU10613-48T 48T
EUR 571.55

Mouse Advanced Oxidation Protein Products (AOPP) ELISA Kit

EKU10613-5x96T 5x96T
EUR 3878.38

Mouse Advanced Oxidation Protein Products (AOPP) ELISA Kit

EKU10613-96T 96T
EUR 816.5

Mouse Advanced Oxidation Protein Products (AOPP) ELISA Kit

EKN51724-48T 48T
EUR 355.6

Mouse Advanced Oxidation Protein Products (AOPP) ELISA Kit

EKN51724-5x96T 5x96T
EUR 2413

Mouse Advanced Oxidation Protein Products (AOPP) ELISA Kit

EKN51724-96T 96T
EUR 508

Mouse advanced oxidation protein products(AOPP)ELISA Kit

GA-E0937MS-48T 48T
EUR 403.2

Mouse advanced oxidation protein products(AOPP)ELISA Kit

GA-E0937MS-96T 96T
EUR 640.8

Mouse Advanced Oxidation Protein Products (AOPP) ELISA Kit

20-abx585219
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Advanced oxidation protein products (AOPP) ELISA Kit

NSL1650m 96 Test
EUR 528

Mouse advanced oxidation protein products,AOPP ELISA Kit

SL0737Mo -
EUR 528

Mouse advanced oxidation protein products,AOPP ELISA Kit

YLA0017MO-48T 48T Ask for price

Mouse advanced oxidation protein products,AOPP ELISA Kit

YLA0017MO-96T 96T Ask for price

Human advanced oxidation Protein products, AOPP GENLISA ELISA

KBH1266 1 x 96 wells
EUR 286

Bovine Advanced oxidation protein products(AOPP)ELISA Kit

NSL1642Bo 96 T
EUR 528

Canine Advanced oxidation protein products(AOPP) ELISA Kit

NSL1788Ca 1883 wells
EUR 528

Human Advanced Oxidation Protein Products (AOPP) CLIA Kit

20-abx490387
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Porcine Advanced oxidation protein products(AOPP) ELISA Kit

NSL1480Po 96T
EUR 528

Chicken Advanced oxidation protein products(AOPP) ELISA Kit

NSL1469Ch 1564 T
EUR 528

Human Advanced Oxidation Protein Products (AOPP) Protein

20-abx651587
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Advanced Oxidation Protein Products (AOPP) Protein

abx651587-100g 100 µg
EUR 400

Human Advanced Oxidation Protein Products (AOPP) Protein

abx651587-10g 10 µg
EUR 250

Human Advanced Oxidation Protein Products (AOPP) Protein

abx651587-50g 50 µg
EUR 337.5

ELISA kit for Rat Advanced oxidation protein products (AOPP)

KTE100979-48T 48T
EUR 398.4
Description: Quantitative sandwich ELISA for measuring Rat Advanced oxidation protein products (AOPP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat Advanced oxidation protein products (AOPP)

KTE100979-5platesof96wells 5 plates of 96 wells
EUR 2538
Description: Quantitative sandwich ELISA for measuring Rat Advanced oxidation protein products (AOPP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat Advanced oxidation protein products (AOPP)

KTE100979-96T 96T
EUR 646.8
Description: Quantitative sandwich ELISA for measuring Rat Advanced oxidation protein products (AOPP) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Guinea pig Advanced oxidation protein products(AOPP) ELISA Kit

NSL1432Gp 96T
EUR 528

Native Advanced Oxidation Protein Products (AOPP)

RPU55157-100ug 100ug
EUR 348.7

Native Advanced Oxidation Protein Products (AOPP)

RPU55157-1mg 1mg
EUR 1372.8
CONCLUSIONS
When adopted by pathogen detection utilizing PCR and affirmation by amplicon hybridization, detection limits of 10(3) and 10(1) CFU per 11-g pattern had been achieved for L. monocytogenes and serovar. Enteritidis, respectively, in each product varieties and with out prior cultural enrichment.
Nicholas

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Nicholas

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