Posts Tagged: equipmentshare website

Optimized methodology for product recovery following emulsion PCR: applications for amplification of aptamer libraries and other complex templates.

Bias and background points make environment friendly amplification of complicated template mixes similar to aptamer and genomic DNA libraries through standard PCR strategies tough; emulsion PCR is being more and more utilized in such situations to avoid these issues. Nonetheless, earlier than merchandise generated through emulsion PCR can be utilized in downstream workflows, they must be recovered from the […]

Evaluating putative chimeric sequences from PCR-amplified products.

BACKGROUND PCR amplification of extremely homologous genes from complicated DNA mixtures is understood to generate a major proportion of chimeric sequences. Ribosomal RNA genes are used for microbial species detection and identification in pure environments, and present assessments of microbial range are based mostly on these sequences. Thus, chimeric sequences may result in the invention […]