Mi-iuy croaker (Miichthys miiuy) is among the most necessary substances of Korean delicacies and thus has the best financial worth. Nevertheless, the same morphological traits amongst croaker fish belonging to household Sciaenidae are sometimes exploited for seafood fraud. On this research, M. miiuy-specific primer set was designed and additional improved by the event of a speedy and cost-effective duplex PCR methodology. The specificity of M. miiuy-specific duplex PCR was examined utilizing 22 seafood species, and no cross-reactivity was noticed. The sensitivity of the PCR assay was discovered to be 0.1 ng/µL. For the primary time, labeling compliance of 43 business m-iuy croaker merchandise was verified utilizing each full DNA barcoding and M. miiuy-specific duplex PCR strategies. For species identification, BOLDSYSTEMS and GenBank database had been screened with the consensus sequences of every PCR product as a question. This identification end result was additional confirmed utilizing the M. miiuy-specific duplex PCR methodology.
The findings of this research revealed that principal species substituted had been regulation croaker , bigeye croaker, whitemouth croaker, and tigertoothed croaker. A big share (21%) of mislabeling was current in business mi-iuy merchandise offered on the South Korea market. Authentication of dairy and meat merchandise is necessary to make sure truthful competitors, shopper profit, and meals security. The big distinction in worth between camel and cow milk could also be an incentive to adulterate camel dairy merchandise with cow-derived foodstuffs. Nevertheless, no research up to now have used triplex real-time PCR with an endogenous management to establish camel and cow origins in dairy and meat merchandise. On this research, we developed a triplex real-time PCR assay based mostly on amplification of mitochondrial 12S ribosomal DNA for the authentication of camel-derived dairy and meat merchandise.
This methodology was utilized to establish camel and cow DNA in milk, yogurt, cheese, milk powder, milk beverage, meat merchandise, and mixtures with milk and meat. Concentrations as little as 1 to five% and 0.1% camel milk and meat, respectively, had been detected within the mixtures, and 1 to five% and 0.1% cow milk and meat, respectively, had been recognized through this method.
Authentication of Ginkgo biloba Natural Merchandise by a Novel Quantitative Actual-Time PCR Strategy
Ginkgo biloba is a broadly used medicinal plant. As a consequence of its potential therapeutic results, it’s an ingredient in a number of natural merchandise, resembling plant infusions and plant meals dietary supplements (PFS). At present, ginkgo is among the hottest botanicals utilized in PFS. As a consequence of their recognition and excessive price, ginkgo-containing merchandise are susceptible to be fraudulently substituted by different plant species. Subsequently, this work aimed toward creating a way for G. biloba detection and quantification. A brand new inner transcribe spacer (ITS) marker was recognized, permitting the event of a ginkgo-specific real-time polymerase chain response (PCR) assay concentrating on the ITS area, with excessive specificity and sensitivity, right down to 0.02 pg of DNA.
Moreover, a normalized real-time PCR method utilizing the delta cycle quantification (ΔCq) methodology was proposed for the efficient quantification of ginkgo in plant mixtures. The strategy exhibited excessive efficiency parameters, particularly PCR effectivity, coefficient of correlation and lined dynamic vary (50-0.01%), reaching limits of detection and quantification of 0.01% (w/w) of ginkgo in tea plant (Camellia sinensis). The quantitative method was efficiently validated with blind mixtures and additional utilized to business ginkgo-containing natural infusions. The estimated ginkgo contents of plant combination samples recommend adulterations on account of discount or virtually elimination of ginkgo.
On this work, helpful and sturdy instruments had been proposed to detect/quantify ginkgo in natural merchandise, which suggests the necessity for a more practical and stricter management of such merchandise.
Identification and authentication of commercial mi-iuy croaker (Miichthys miiuy) products by two PCR-based methods
Comparability of Actual-Time PCR Quantification Strategies within the Identification of Poultry Species in Meat Merchandise
Poultry meat is consumed worldwide and is susceptible to meals fraud due to giant worth variations amongst meat from totally different poultry species. Exact and delicate analytical strategies are crucial to regulate poultry meat merchandise. We selected species-specific sequences of the cytochrome b gene to develop two multiplex real-time polymerase chain response (real-time PCR) programs: one for rooster (Gallus gallus), guinea fowl (Numida meleagris), and pheasant (Phasianus colchicus), and one for quail (Coturnix japonica) and turkey (Meleagris gallopavo). For every species, added meat may very well be detected right down to 0.5 % w/w. No cross reactions had been seen.
For these two real-time PCR programs, we utilized three totally different quantification strategies: (A) with relative normal curves, (B) with matrix-specific multiplication elements, and (C) with an inner DNA reference sequence to normalize and to regulate inhibition. All three quantification strategies had affordable restoration charges from 43% to 173%. Methodology B had extra accepted restoration charges, i.e., within the vary 70-130%, particularly 83% in comparison with 75% for methodology A or C. The polymerase chain response is just not solely important for a lot of DNA-based diagnostic strategies however can be exploited in different molecular strategies that require an upstream amplification step. Multiplex PCRs are particularly enticing as they cut back the variety of particular person reactions. Nevertheless, the multiplexing effectivity is impaired by primer interactions such because the formation of primer dimers.
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EUR 176 |
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PCR-96-C-S |
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EUR 183 |
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EUR 202 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
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R-96-PCR-FY |
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5/pk |
EUR 119 |
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Description: Micro Tubes; Racks Tubes - Axygen |
 384 WELL BLACK RIGI PLATE PCR MICROPLATE |
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PCR-384-RGD-BK |
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20/pk |
EUR 488 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
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PCR-96M2-HS-BK |
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EUR 187 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
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PCR-96M2-HS-C |
CORNING |
10/pk |
EUR 167 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
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PCR-96M2-HS-R |
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10/pk |
EUR 195 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
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CORNING |
10/pk |
EUR 195 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
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PCR-960-LP-S2 |
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EUR 144.25 |
- Product category: Labware/Multiwell Plates/96-Well
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) 96-well GE/PCR Clean Up Purification Kit (1 plates) |
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FAPKE-96001 |
Favorgen |
1 prep |
EUR 146 |
) 96-well GE/PCR Clean Up Purification Kit (2 plates) |
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FAPKE-96002 |
Favorgen |
2 preps |
EUR 188 |
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FAPKE-96004 |
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4 preps |
EUR 274 |
 Adapter for 96 well plate |
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3510147 |
Atto |
1unit |
EUR 5581 |
 Streptavidin coated 96-well Plate |
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6523-5 |
Biovision |
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EUR 327 |
 PCR 96-Plate unskirted |
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A28239 |
GeneOn |
25 pcs |
EUR 98 |
 384 WELL CLEAR RIGI PLATE PCR MICROPLATE. CLEAR |
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PCR-384-RGD-C |
CORNING |
20/pk |
EUR 459 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
 SINGLE BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED |
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480130 |
CORNING |
1/pk |
EUR 223 |
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Description: Lab Equipment; Constant Temperature Equipment |
 DUAL BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED |
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480132 |
CORNING |
1/pk |
EUR 218 |
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Description: Lab Equipment; Constant Temperature Equipment |
 Adaptor for 96-well PCR plate, accessory for AgileSealer? |
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APS-200-96PCR |
ACTGene |
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EUR 351 |
 96 Well PCR Plates, Fits ABI Fast Plate Machines |
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GP615 |
GenDepot |
25 plates |
EUR 212 |
) 96 well filter plate (960ul each well) |
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SD5006 |
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EUR 96.54 |
- Product category: Labware/Filtration Supplies/Filter Plates - DNA
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) 96-well GEL Purification Kit (1 prep) |
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FAGKE-96001 |
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EUR 158 |
) 96-well GEL Purification Kit (2 prep) |
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FAGKE-96002 |
Favorgen |
2 preps |
EUR 213 |
) 96-well GEL Purification Kit (4 prep) |
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4 preps |
EUR 317 |
 EZ-10 Spin Column PCR Products Purification Kit |
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BS363 |
Bio Basic |
50Preps |
EUR 76.64 |
- Product category: Molecular Biology Kits/DNA - Extraction/PCR Products
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EZ-10 Spin Column PCR Products Purification Kit |
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BS364 |
Bio Basic |
100preps |
EUR 95.68 |
- Product category: Molecular Biology Kits/DNA - Extraction/PCR Products
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EUR 166.58 |
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 96 WELL FLUORESCENT PCR STORAGE RACK IN SPECTRUM PACK. |
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R-96-PCR-FSP |
CORNING |
5/pk |
EUR 119 |
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Description: Micro Tubes; Racks Tubes - Axygen |
 96-Well Plate DNA Cleanup Miniprep Kit |
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BS369 |
Bio Basic |
5XPlates, 480prep |
EUR 250.31 |
- Product category: Molecular Biology Kits/DNA - Extraction/Clean-up
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96-Well Plate DNA Cleanup Miniprep Kit |
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BS3692 |
Bio Basic |
2xPlates, 192prep |
EUR 155.16 |
- Product category: Molecular Biology Kits/DNA - Extraction/Clean-up
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 96-Well Plate Plasmid DNA Miniprep Kit |
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BS415 |
Bio Basic |
5XPlates, 480prep |
EUR 250.31 |
- Product category: Molecular Biology Kits/DNA - Extraction/Bacteria
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96-Well Plate Plasmid DNA Miniprep Kit |
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Bio Basic |
2XPlates, 192prep |
EUR 155.16 |
- Product category: Molecular Biology Kits/DNA - Extraction/Bacteria
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 96-Well Plate Viral DNA Miniprep Kit |
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VT92032 |
Bio Basic |
2XPlates, 192prep |
EUR 413.22 |
- Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Viral
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 1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY |
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P-DW-12-HC |
CORNING |
5/pk |
EUR 152 |
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Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen |
) Polystyrene-bottomed 96-well plate (ELISA) |
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CT361 |
U-CyTech |
6 |
EUR 73 |
 Clear Coated 96 Well Plate, 1EA |
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C107-1EA |
Arbor Assays |
1EA |
EUR 189 |
) 96 well filter plate (no filter) |
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SD5006-EMPTY |
Bio Basic |
12UNIT |
EUR 87.41 |
- Product category: Labware/Filtration Supplies/Filter Plates - DNA
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 384 Well Plate, 10/Bag |
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PCR-384-SN |
Bio Basic |
1PK, 10UNIT |
EUR 93.5 |
- Product category: Labware/Multiwell Plates/384-Well
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 96 WELL CLEAR LOW PROFILE PCR MICROPLATE FOR ABI |
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PCR-96-LP-AB-C |
CORNING |
25/pk |
EUR 388 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
 96 WELL WHITE LOW PROFILE PCR MICROPLATE FOR AB |
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PCR-96-LP-AB-W |
CORNING |
25/pk |
EUR 388 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
 96 WELL CLEAR LOW PROFILE, FLAT TOP PCR MICROPLATE |
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PCR-96-LP-FLT-C |
CORNING |
25/pk |
EUR 230 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
 96 WELL WHITE LOW PROFILE, FLAT TOP PCR MICROPLATE |
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PCR-96-LP-FLT-W |
CORNING |
25/pk |
EUR 230 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
 DUAL BLOCK, 384 WELL PCR PLATE |
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480133 |
CORNING |
1/pk |
EUR 231 |
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Description: Lab Equipment; Constant Temperature Equipment |
 96 WELL SITTING DROP HIGH THROUGHPUT CRYSTALLOGRAPHY PLATE. |
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CP-AXYGEM-96-50 |
CORNING |
10/pk |
EUR 457 |
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Description: Crystallography Plates; Crystallography Plates - Axygen |
 96 WELL CLEAR PCR HALF SKIRT SINGLE BAR-CODED AMPLIFICATION PLATE WITH A SINGLE NOTCH. |
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PCR-96M2-HS-C-BC |
CORNING |
10/pk |
EUR 229 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
 96 WELL BARCODED H1-H12, CLEAR PCR HALF SKIRT AMPLIFICATION PLATE WITH A SINGLE NOTCH |
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PCR-96M2-HS-C-PBC |
CORNING |
10/pk |
EUR 227 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
) 96 well DNA plate with membrane (960ul each well) |
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SD5007 |
Bio Basic |
12UNIT |
EUR 242.7 |
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) 96 well RNA plate with membrane (960ul each well) |
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SD5009 |
Bio Basic |
12UNIT |
EUR 260.97 |
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 SEALING MAT, FOR 96 WELL PCR MICROPLATES, SILICONE, NATURAL, NONSTERILE, BULK |
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AM-96-PCR-RD |
CORNING |
10/pk |
EUR 164 |
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Description: Sealing Products; Sealing mats - Axygen |
) 96-well Plasmid DNA Extraction Kit (1 plate) |
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FAPWE-96001 |
Favorgen |
1 prep |
EUR 146 |
 96 Well Plate RNA Cleanup and Concentration Kit |
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BRC1251 |
Bio Basic |
2xPlates, 192prep |
EUR 250.31 |
- Product category: Molecular Biology Kits/RNA - Extraction/Clean-up
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 96-Well Plate Animal Genomic DNA Miniprep Kit |
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BS437 |
Bio Basic |
5XPlates, 480prep |
EUR 362.98 |
- Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal
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96-Well Plate Animal Genomic DNA Miniprep Kit |
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BS4372 |
Bio Basic |
2XPlates, 192prep |
EUR 195.5 |
- Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Animal
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 96 Well Plate Yeast Genomic DNA Miniprep Kit |
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BS8357 |
Bio Basic |
2XPlates, 192prep |
EUR 195.5 |
- Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Yeast
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 96-Well Plate Plant Genomic DNA Miniprep Kit |
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BS8361 |
Bio Basic |
2XPlates, 192prep |
EUR 195.5 |
- Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Plant
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 96 Well Plate Blood Genomic DNA Miniprep Kit |
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BT92031 |
Bio Basic |
2Plates, 192prep |
EUR 175.71 |
- Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Blood
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96 Well Plate Blood Genomic DNA Miniprep Kit |
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BT92032 |
Bio Basic |
5Plates, 480prep |
EUR 324.92 |
- Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Blood
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 96-Well Plate Bacteria Genomic DNA Miniprep Kit |
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SK1292 |
Bio Basic |
2XPlates, 192prep |
EUR 175.71 |
- Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria
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96-Well Plate Bacteria Genomic DNA Miniprep Kit |
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SK1295 |
Bio Basic |
5XPlates, 480prep |
EUR 324.92 |
- Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Bacteria
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 PCR Purification Kit |
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20-abx098084 |
Abbexa |
|
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- Shipped within 5-10 working days.
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 96 Well Breast Tumor Genomic DNA Plate |
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D8235086-1 |
Biochain |
1 plate |
EUR 316 |
 96 Well Colon Tumor Genomic DNA Plate |
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D8235090-1 |
Biochain |
1 plate |
EUR 316 |
 96 Well Lung Tumor Genomic DNA Plate |
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D8235152-1 |
Biochain |
1 plate |
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 96 Well Stomach Tumor Genomic DNA Plate |
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D8235248-1 |
Biochain |
1 plate |
EUR 316 |
) Polystyrene-bottomed 96-well plate + lid (ELISPOT) |
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CT365 |
U-CyTech |
5 |
EUR 78 |
 2 x Clear 96 well Plate, 2EA |
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X003-2EA |
Arbor Assays |
2EA |
EUR 65 |
 5 x Clear 96 well Plate, 5EA |
|
X003-5EA |
Arbor Assays |
5EA |
EUR 80 |
2 x Clear 96 well Plate, 2EA |
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X018-2EA |
Arbor Assays |
2EA |
EUR 65 |
 4 x Clear 96 Well Plate, 4EA |
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X018-4EA |
Arbor Assays |
4EA |
EUR 87 |
 Black Half Area 96 Well Plate, 1EA |
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X023-1EA |
Arbor Assays |
1EA |
EUR 80 |
 PCR 96-Plate unskirted, low profile |
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A28240 |
GeneOn |
25 pcs |
EUR 98 |
 PCR 96-Plate skirted, low profile |
|
A28241 |
GeneOn |
25 pcs |
EUR 100 |
 48 WELL PCR MICROPLATE |
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PCR-48-C |
CORNING |
10/pk |
EUR 102 |
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Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen |
 96 WELL HALF SKIRT AUTOMATION COMPATIBLE PCR MICROPLATE, NON-STERILE, BLUE |
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PCR-96-HS-AC-B |
CORNING |
10/pk |
EUR 170 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
 96 WELL HALF SKIRT AUTOMATION COMPATIBLE PCR MICROPLATE, NON-STERILE, CLEAR |
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PCR-96-HS-AC-C |
CORNING |
10/pk |
EUR 170 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
 96 WELL HALF SKIRT AUTOMATION COMPATIBLE PCR MICROPLATE, NON-STERILE, GREEN |
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PCR-96-HS-AC-G |
CORNING |
10/pk |
EUR 170 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
 96 WELL HALF SKIRT AUTOMATION COMPATIBLE PCR MICROPLATE, NON-STERILE, RED |
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PCR-96-HS-AC-R |
CORNING |
10/pk |
EUR 170 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
 96 WELL HALF SKIRT AUTOMATION COMPATIBLE PCR MICROPLATE, NON-STERILE, WHITE |
|
PCR-96-HS-AC-W |
CORNING |
10/pk |
EUR 170 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
 96 WELL HALF SKIRT AUTOMATION COMPATIBLE PCR MICROPLATE, NON-STERILE, YELLOW |
|
PCR-96-HS-AC-Y |
CORNING |
10/pk |
EUR 170 |
|
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
 1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY PRE-STERILIZED |
|
P-DW-12-HC-S |
CORNING |
5/pk |
EUR 207 |
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Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen |
) SpotLiter? 96 well Plate Light Tracker (foot pedal & USB included) |
|
ACT-SL-96 |
ACTGene |
|
EUR 647 |
 96 Well Plate Bacterial Total RNA Miniprep Super Kit |
|
BS585 |
Bio Basic |
5XPlates, 480prep |
EUR 487.82 |
- Product category: Molecular Biology Kits/RNA - Extraction/Bacteria
|
On this research, covalent crosslinking of primers through their 5′-ends was used to keep away from these undesired results. The specificity of the primers in addition to the effectivity of the PCR may very well be elevated upon primer crosslinking in reactions containing as much as 34 primer pairs concentrating on crucial antibiotic resistance genes in a single multiplex response.
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