Identification and authentication of commercial mi-iuy croaker (Miichthys miiuy) products by two PCR-based methods

Mi-iuy croaker (Miichthys miiuy) is among the most necessary substances of Korean delicacies and thus has the best financial worth. Nevertheless, the same morphological traits amongst croaker fish belonging to household Sciaenidae are sometimes exploited for seafood fraud. On this research, M. miiuy-specific primer set was designed and additional improved by the event of a speedy and cost-effective duplex PCR methodology. The specificity of M. miiuy-specific duplex PCR was examined utilizing 22 seafood species, and no cross-reactivity was noticed. The sensitivity of the PCR assay was discovered to be 0.1 ng/µL. For the primary time, labeling compliance of 43 business m-iuy croaker merchandise was verified utilizing each full DNA barcoding and M. miiuy-specific duplex PCR strategies. For species identification, BOLDSYSTEMS and GenBank database had been screened with the consensus sequences of every PCR product as a question. This identification end result was additional confirmed utilizing the M. miiuy-specific duplex PCR methodology.

The findings of this research revealed that principal species substituted had been regulation croaker , bigeye croaker, whitemouth croaker, and tigertoothed croaker. A big share (21%) of mislabeling was current in business mi-iuy merchandise offered on the South Korea market. Authentication of dairy and meat merchandise is necessary to make sure truthful competitors, shopper profit, and meals security. The big distinction in worth between camel and cow milk could also be an incentive to adulterate camel dairy merchandise with cow-derived foodstuffs. Nevertheless, no research up to now have used triplex real-time PCR with an endogenous management to establish camel and cow origins in dairy and meat merchandise. On this research, we developed a triplex real-time PCR assay based mostly on amplification of mitochondrial 12S ribosomal DNA for the authentication of camel-derived dairy and meat merchandise.

This methodology was utilized to establish camel and cow DNA in milk, yogurt, cheese, milk powder, milk beverage, meat merchandise, and mixtures with milk and meat. Concentrations as little as 1 to five% and 0.1% camel milk and meat, respectively, had been detected within the mixtures, and 1 to five% and 0.1% cow milk and meat, respectively, had been recognized through this method.

 

Authentication of Ginkgo biloba Natural Merchandise by a Novel Quantitative Actual-Time PCR Strategy

Ginkgo biloba is a broadly used medicinal plant. As a consequence of its potential therapeutic results, it’s an ingredient in a number of natural merchandise, resembling plant infusions and plant meals dietary supplements (PFS). At present, ginkgo is among the hottest botanicals utilized in PFS. As a consequence of their recognition and excessive price, ginkgo-containing merchandise are susceptible to be fraudulently substituted by different plant species. Subsequently, this work aimed toward creating a way for G. biloba detection and quantification. A brand new inner transcribe spacer (ITS) marker was recognized, permitting the event of a ginkgo-specific real-time polymerase chain response (PCR) assay concentrating on the ITS area, with excessive specificity and sensitivity, right down to 0.02 pg of DNA.
Moreover, a normalized real-time PCR method utilizing the delta cycle quantification (ΔCq) methodology was proposed for the efficient quantification of ginkgo in plant mixtures. The strategy exhibited excessive efficiency parameters, particularly PCR effectivity, coefficient of correlation and lined dynamic vary (50-0.01%), reaching limits of detection and quantification of 0.01% (w/w) of ginkgo in tea plant (Camellia sinensis). The quantitative method was efficiently validated with blind mixtures and additional utilized to business ginkgo-containing natural infusions. The estimated ginkgo contents of plant combination samples recommend adulterations on account of discount or virtually elimination of ginkgo.
On this work, helpful and sturdy instruments had been proposed to detect/quantify ginkgo in natural merchandise, which suggests the necessity for a more practical and stricter management of such merchandise.
 Identification and authentication of commercial mi-iuy croaker (Miichthys miiuy) products by two PCR-based methods

Identification and authentication of commercial mi-iuy croaker (Miichthys miiuy) products by two PCR-based methods

Comparability of Actual-Time PCR Quantification Strategies within the Identification of Poultry Species in Meat Merchandise

Poultry meat is consumed worldwide and is susceptible to meals fraud due to giant worth variations amongst meat from totally different poultry species. Exact and delicate analytical strategies are crucial to regulate poultry meat merchandise. We selected species-specific sequences of the cytochrome b gene to develop two multiplex real-time polymerase chain response (real-time PCR) programs: one for rooster (Gallus gallus), guinea fowl (Numida meleagris), and pheasant (Phasianus colchicus), and one for quail (Coturnix japonica) and turkey (Meleagris gallopavo). For every species, added meat may very well be detected right down to 0.5 % w/w. No cross reactions had been seen.
For these two real-time PCR programs, we utilized three totally different quantification strategies: (A) with relative normal curves, (B) with matrix-specific multiplication elements, and (C) with an inner DNA reference sequence to normalize and to regulate inhibition. All three quantification strategies had affordable restoration charges from 43% to 173%. Methodology B had extra accepted restoration charges, i.e., within the vary 70-130%, particularly 83% in comparison with 75% for methodology A or C. The polymerase chain response is just not solely important for a lot of DNA-based diagnostic strategies however can be exploited in different molecular strategies that require an upstream amplification step. Multiplex PCRs are particularly enticing as they cut back the variety of particular person reactions. Nevertheless, the multiplexing effectivity is impaired by primer interactions such because the formation of primer dimers.

96-well GE/PCR Clean Up Purification Kit (2 plates)

FAPKE-96002 2 preps
EUR 225.6

96-well GE/PCR Clean Up Purification Kit (4 plates)

FAPKE-96004 4 preps
EUR 328.8

96-well GE/PCR Clean Up Purification Kit (1 plates)

FAPKE_96001 1 kit
EUR 36

96-well GE/PCR Clean Up Purification Kit (2 plates)

FAPKE_96002 1 kit
EUR 68.5

96-well GE/PCR Clean Up Purification Kit (4 plates)

FAPKE_96004 1 kit
EUR 134.5

HiPurA® 96 PCR Product Purification Kit

MB519-1X96PR 1 unit
EUR 145.13
Description: HiPurA® 96 PCR Product Purification Kit

illustra GFx 96 Purification Kit 10 x 96 well plates - EACH

28903445 EACH
EUR 2103.3

HiPurA® PCR Product Purification Kit

MB512-20PR 1 unit
EUR 29.23
Description: HiPurA® PCR Product Purification Kit

HiPurA® PCR Product Purification Kit

MB512-250PR 1 unit
EUR 259.94
Description: HiPurA® PCR Product Purification Kit

HiPurA® PCR Product Purification Kit

MB512-50PR 1 unit
EUR 62.37
Description: HiPurA® PCR Product Purification Kit

PCR Purification Solutions Kit (for 50x 96 well-plates)

9K-006-0005 50, 100ug
EUR 541.8

96-well GEL Purification Kit (1 prep)

FAGKE-96001 1 prep
EUR 189.6

96-well GEL Purification Kit (2 prep)

FAGKE-96002 2 preps
EUR 255.6

96-well GEL Purification Kit (4 prep)

FAGKE-96004 4 preps
EUR 380.4

96-well GEL Purification Kit (1 prep)

FAGKE_96001 1 kit
EUR 45

96-well GEL Purification Kit (2 prep)

FAGKE_96002 1 kit
EUR 87.5

96-well GEL Purification Kit (4 prep)

FAGKE_96004 1 kit
EUR 168

HiPer® PCR Product Purification Teaching

HTBM041-10PR 1 unit
EUR 19
Description: HiPer® PCR Product Purification Teaching

HiPurA® PCR Product and Gel Purification

MB563-20PR 1 unit
EUR 54.58
Description: HiPurA® PCR Product and Gel Purification

HiPurA® PCR Product and Gel Purification

MB563-250PR 1 unit
EUR 406.05
Description: HiPurA® PCR Product and Gel Purification

HiPurA® PCR Product and Gel Purification

MB563-50PR 1 unit
EUR 109.02
Description: HiPurA® PCR Product and Gel Purification

PCR Purification Kit

20-abx098084
  • Ask for price
  • Ask for price
  • 200 rxns
  • 50 rxns

PCR Purification Kit

abx098084-100l 100 µl
EUR 250

PCR Purification Kit

abx098084-1ml 1 ml Ask for price

PCR Purification Kit

abx098084-200l 200 µl
EUR 337.5

PURY PCR Purification Kit

P2004-100 100 Prep
EUR 149

PURY PCR Purification Kit

P2071-100 100 Prep Ask for price

Zenoquick PCR Purification Kit

Z3002-050 50 preps
EUR 262.8

Zenoquick PCR Purification Kit

Z3002-200 4x50 preps
EUR 757.2

PCR Purification Kit (100 preps)

9K-006-0003 100preps
EUR 268.8

PCR Purification Kit (200 preps)

9K-006-0004 200preps, 200prep
EUR 373.2

PCR Purification Kit - Column Kit

PP-201L 250preparations
EUR 251.2

PCR Purification Kit - Column Kit

PP-201S 50preparations
EUR 64.9

PURY PCR & Gel Purification Kit

P2006-100 100 Prep
EUR 160

SeqPure PCR Purification Kit, 500 ml

Z1510003 500 ml
EUR 6712

Gel and PCR DNA Purification kit

K1455-250 250 Preps
EUR 576

Gel and PCR DNA Purification kit

K1455-50 50 Preps
EUR 247.2

SeqPure PCR Purification Kit, 5 ml

Z1510001 5 ml
EUR 291

SeqPure PCR Purification Kit, 50 ml

Z1510002 50 ml
EUR 971

MicroElute GEL/PCR Purification Kit (200prep)

FAEPK-001-1 200 preps
EUR 249.6

MicroElute GEL/PCR Purification Kit (300prep)

FAEPK-001-2 300 preps
EUR 274.8

PCR DNA extraction and purification kit

K1444-100 each
EUR 405.6

MicroElute GEL/PCR Purification Kit (50prep)

FAEPK-001 50 preps
EUR 157.2

GEL/PCR Purification Mini Kit (100prep)

FAGCK-001 100 preps
EUR 154.8

GEL/PCR Purification Mini Kit (300prep)

FAGCK-001-1 300 preps
EUR 206.4

GEL/PCR Purification Mini Kit (100prep)

FAGCK_001 1 kit
EUR 22.5

GEL/PCR Purification Mini Kit (300prep)

FAGCK_001-1 1 kit
EUR 78.5

GEL/PCR Purification Mini Kit (100prep)

APGCK_100 100 extractions
EUR 20

GEL/PCR Purification Mini Kit (300prep)

APGCK_300 300 extractions
EUR 50

HiPurA® 96 Plasmid DNA Purification Kit

MB518-1X96PR 1 unit
EUR 153.64
Description: HiPurA® 96 Plasmid DNA Purification Kit

PCR DNA Extraction and Purification

EP10013 100 Tests
EUR 356.4

T-Pro Gel/PCR DNA Purification Mini Kit (100)

RB94-NES100 100preps/Kit
EUR 193.2

T-Pro Gel/PCR DNA Purification Mini Kit (250)

RB94-NES250 250preps/Kit
EUR 266.4

HiPurA™ 96 Total RNA Purification Kit

MB612-1X96PR 1 unit
EUR 220.5
Description: HiPurA™ 96 Total RNA Purification Kit

T-Pro Gel/PCR DNA Purification Maxi Kit (10)

RB94-NEL010 10preps/Kit
EUR 193.2

PCR Purification Kit, Centrifugal Colμmn Method, 50 reactions/pk

208101 8 pcs/cs
EUR 22.55
Description: PCR Purification Kit, Centrifugal Colμmn Method, 50 reactions/pk

RNA Purification Kit

abx098095-25rxns 25 rxns
EUR 276

RNA Purification Kit

MB1006-250preps 250 preps
EUR 476.4

RNA Purification Kit

MB1006-50preps 50 preps
EUR 199.2

RNA Purification Kit

abx098095-100l 100 µl
EUR 300

RNA Purification Kit

abx098095-1ml 1 ml Ask for price

RNA Purification Kit

abx098095-200l 200 µl Ask for price

illustra GFx PCR DNA and Gel Band Purification Kit 100 prep - EACH

28903470 EACH
EUR 275.4

Plant MicroRNA Purification Kit

10760135-1 25 Prep(s)
EUR 245.48

Plant RNA/DNA Purification Kit

10760069-1 50 Prep(s)
EUR 259.68

MicroElute GEL/PCR Purification Kit (100prep) (blister packing for columns)

FAEPK_001-1B 1 kit
EUR 64.96

Lysosome Purification Kit

K235-50 each
EUR 601.2

Adenovirus Purification Kit

K1459-100 100 Preps
EUR 1244.4

Adenovirus Purification Kit

K1459-20 20 Preps
EUR 727.2

PURY Gel Purification Kit

P2005-100 100 Prep
EUR 149

Pury Viral RNA Purification Kit for RT-PCR Detection

P2020-050 50 Prep
EUR 190

Pury Viral RNA Purification Kit for RT-PCR Detection

P2020-100 2x50 Prep
EUR 285

HCV Mini Purification Kit

K1309-10 each
EUR 802.8

HCV Mini Purification Kit

K1309-20 each
EUR 1096.8

HCV Maxi Purification Kit

K1310-10 each
EUR 1683.6

HCV Maxi Purification Kit

K1310-2 each
EUR 639.6

HCV Maxi Purification Kit

K1310-4 each
EUR 1083.6

MicroElute GEL/PCR Purification Kit (50prep) (blister packing for columns)

FAEPK_001B 1 kit
EUR 33

His-tag Purification Kit

abx299036-4U 4 U
EUR 427.2

Sperm DNA Purification Kit

K1465-250 250 Preps
EUR 661.2

Sperm DNA Purification Kit

K1465-50 50 Preps
EUR 381.6

Stool DNA Purification Kit

K1474-250 250 Preps
EUR 721.2

Stool DNA Purification Kit

K1474-50 50 Preps
EUR 417.6

Saliva DNA Purification Kit

K1468-250 250 Preps
EUR 661.2

Saliva DNA Purification Kit

K1468-50 50 Preps
EUR 381.6

Phosphoprotein Purification Kit

AKR-105 2 preps
EUR 358.8
Description: The Phosphoprotein Purification Kit provides an efficient system for quick purification/enrichment of phosphoproteins from various samples. Phosphorylated proteins are affinity purified from lysates with a single-step purification matrix. The entire procedure takes about 4 hours. Each preparation can handle 2.5 mg of total lysate protein (approx. one confluent 100 mm dish).

Phosphoprotein Purification Kit

AKR-106 5 preps
EUR 582
Description: The Phosphoprotein Purification Kit provides an efficient system for quick purification/enrichment of phosphoproteins from various samples. Phosphorylated proteins are affinity purified from lysates with a single-step purification matrix. The entire procedure takes about 4 hours. Each preparation can handle 2.5 mg of total lysate protein (approx. one confluent 100 mm dish).

PurePrep 96 Nucleic Acid Purification System

AS00001 1
EUR 27000

PureXtract RNA Purification Kit

R6111-050 50 prep
EUR 336

PureXtract RNA Purification Kit

R6111-200 200 prep
EUR 1048.8

Rapid Antibody Purification Kit

AKR-160 10 assays
EUR 622.8
Description: Cell Biolabs? Rapid Antibody Purification kit is designed for rapid, single-step purification of high-quality IgG from ascites, serum and tissue culture media or hybridoma supernatants.

Endo-Low Max Plasmid Purification Kit

EP020 10T
EUR 229

Chromatin DNA purification (100) Kit

TBS8055-100 100 samples
EUR 158

HiPurA® 96 SuperPlant DNA Purification K

MB574-1X96PR 1 unit
EUR 200.76
Description: HiPurA® 96 SuperPlant DNA Purification K

Adenovirus Mini Purification Kit

K1300-10 each
EUR 652.8

Adenovirus Mini Purification Kit

K1300-20 each
EUR 1096.8

Adenovirus Maxi Purification Kit

K1301-10 each
EUR 1554

Adenovirus Maxi Purification Kit

K1301-2 each
EUR 639.6

Adenovirus Maxi Purification Kit

K1301-4 each
EUR 1032

Lentivirus Mini Purification Kit

K1305-10 each
EUR 652.8

Lentivirus Mini Purification Kit

K1305-20 each
EUR 1096.8

Lentivirus Maxi Purification Kit

K1306-10 each
EUR 1554

Lentivirus Maxi Purification Kit

K1306-2 each
EUR 639.6

Lentivirus Maxi Purification Kit

K1306-4 each
EUR 1032

Retrovirus Mini Purification Kit

K1307-10 each
EUR 652.8

Retrovirus Mini Purification Kit

K1307-20 each
EUR 1096.8

Retrovirus Maxi Purification Kit

K1308-10 each
EUR 1554

Retrovirus Maxi Purification Kit

K1308-2 each
EUR 639.6

Retrovirus Maxi Purification Kit

K1308-4 each
EUR 1032

Ethanol, DEPC Treated 96% , For RNA Purification

E4500-050 500ml Ask for price

Protein A Antibody Purification Kit

K974-10 each
EUR 418.8

Mitochondrial DNA Purification Kit

K389-25 each
EUR 470.4

Buccal Swab DNA Purification Kit

K1466-250 250 Preps
EUR 661.2

Buccal Swab DNA Purification Kit

K1466-50 50 Preps
EUR 381.6

ViraBind™ AAV Purification Kit

VPK-140 10 preps
EUR 630

PURY Agarose Gel Purification Kit

P2072-100 100 Prep Ask for price

GST-tagged Protein Purification Kit

EA-TP-K004 10T
EUR 392
Description: Protein Purification

Chromatin DNA purification (50) Kit

TBS8055-50 50 samples
EUR 98

Bacterial DNA Purification Kit II

K1457-250 250 Preps
EUR 697.2

Bacterial DNA Purification Kit II

K1457-50 50 Preps
EUR 381.6

Plasmid DNA extraction and purification kit

K1445-100 each
EUR 444

Total RNA Purification Kit - Column Kit

PP-210L 250preparations
EUR 519.5

Total RNA Purification Kit - Column Kit

PP-210S 50preparations
EUR 129.9

IsoPure™ 96, Automated Purification System, 115V

AP1096 1 each
EUR 47250

IsoPure™ 96, Automated Purification System, 230V

AP1096-E 1 each
EUR 47250

MicroElute GEL Purification Kit (200prep)

FAMGK-001-1 200 preps
EUR 224.4

Genorise® Gel DNA Purification Mini Kit-PCR Clean Kit- 100

GR101080-100 100
EUR 267

Genorise® Gel DNA Purification Mini Kit-PCR Clean Kit- 200

GR101081 200
EUR 477

Genorise® Gel DNA Purification Mini Kit-PCR Clean Kit- 500

GR101081-500 500
EUR 1074

EZCatch? GST-Spin Purification Kit

K861-10 each
EUR 288

Zenoprep Serum DNA Purification Kit

Z2005-000 Adjustable
EUR 574.8

Genorise® Gel DNA Purification Mini Kit-PCR Clean Kit- 50

GR101080 50
EUR 148

HiPurA® DNA/RNA Purification Kit

MB583-20PR 1 unit
EUR 50.25
Description: HiPurA® DNA/RNA Purification Kit

HiPurA® DNA/RNA Purification Kit

MB583-250PR 1 unit
EUR 489.18
Description: HiPurA® DNA/RNA Purification Kit

HiPurA® DNA/RNA Purification Kit

MB583-50PR 1 unit
EUR 106.39
Description: HiPurA® DNA/RNA Purification Kit

Zenoquick Viral DNA Purification Kit

Z2009-050 50 preps
EUR 408

Zenoquick Viral DNA Purification Kit

Z2009-200 4x50 preps
EUR 1129.2

HiPurA® Bone DNA Purification Kit

MB525-10PR 1 unit
EUR 56.1
Description: HiPurA® Bone DNA Purification Kit

HiPurA™ Bone DNA Purification Kit

MB525-20PR 1 unit
EUR 84.96
Description: HiPurA™ Bone DNA Purification Kit

HiPurA® Soil DNA Purification Kit

MB542-20PR 1 unit
EUR 58.38
Description: HiPurA® Soil DNA Purification Kit

HiPurA® Soil DNA Purification Kit

MB542-250PR 1 unit
EUR 372
Description: HiPurA® Soil DNA Purification Kit

HiPurA® Soil DNA Purification Kit

MB542-50PR 1 unit
EUR 116.61
Description: HiPurA® Soil DNA Purification Kit

HiPurA® Food DNA Purification Kit

MB562-20PR 1 unit
EUR 44.02
Description: HiPurA® Food DNA Purification Kit

HiPurA® Food DNA Purification Kit

MB562-250PR 1 unit
EUR 415.6
Description: HiPurA® Food DNA Purification Kit

HiPurA® Food DNA Purification Kit

MB562-50PR 1 unit
EUR 93.28
Description: HiPurA® Food DNA Purification Kit

Zenoprep Genomic DNA Purification Kit

Z2004-400 400 preps
EUR 660

Eye, Nose & Swabs gDNA Purification Kit

K1467-250 250 Preps
EUR 661.2

Eye, Nose & Swabs gDNA Purification Kit

K1467-50 50 Preps
EUR 381.6
On this research, covalent crosslinking of primers through their 5′-ends was used to keep away from these undesired results. The specificity of the primers in addition to the effectivity of the PCR may very well be elevated upon primer crosslinking in reactions containing as much as 34 primer pairs concentrating on crucial antibiotic resistance genes in a single multiplex response.
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