Comparability of Actual-Time PCR Quantification Strategies within the Identification of Poultry Species in Meat Merchandise
Poultry meat is consumed worldwide and is liable to meals fraud due to massive value variations amongst meat from totally different poultry species. Exact and delicate analytical strategies are needed to regulate poultry meat merchandise. We selected species-specific sequences of the cytochrome b gene to develop two multiplex real-time polymerase chain response (real-time PCR) techniques: one for hen (Gallus gallus), guinea fowl (Numida meleagris), and pheasant (Phasianus colchicus), and one for quail (Coturnix japonica) and turkey (Meleagris gallopavo).
For every species, added meat may very well be detected all the way down to 0.5 %Â w/w. No cross reactions had been seen. For these two real-time PCR techniques, we utilized three totally different quantification strategies: (A) with relative commonplace curves, (B) with matrix-specific multiplication elements, and (C) with an inner DNA reference sequence to normalize and to regulate inhibition. All three quantification strategies had affordable restoration charges from 43% to 173%. Methodology B had extra accepted restoration charges, i.e., within the vary 70-130%, particularly 83% in comparison with 75% for technique A or C.
Key phrases:Â hen; guinea fowl; pheasant; quail; quantification; real-time PCR; turkey
Lateral Circulate Genochromatographic Strip for Bare-Eye Detection of Mycobacterium Tuberculosis PCR Merchandise with Gold Nanoparticles as a Reporter
Background: Mycobacterium tuberculosis (MTB) is a pathogen inflicting tuberculosis (TB) in human, and TB may cause huge social and financial disruptions. Lateral circulate take a look at strips (LFTSs) are cheap, moveable, disposable, fast, and easy-to-use analytical instruments.
Goal:Â LFTSs had been ready for the detection of MTB. LFTSs had been fabricated utilizing a brand new particular probe for MTB H37Rv, primarily based on IS6110 sequence gene, and tailed with poly deoxyadenine (dA).
Materials and strategies:Â On this experimental research, to create take a look at and management zones, streptavidin (STP) and a 150-mer dA had been dotted on a nitrocellolose membrane. Gold nanoparticles (GNPs) had been conjugated with poly deoxythymidine sequence and positioned on the conjugate pad.
The composition of immersion buffers for pattern pad and conjugate pad, working answer, options of GNPs-S-dT150 and STP had been launched. DNA genome of MTB and Mycobacterium bovisin scientific samples was amplified with PCR, after which detected by the LFTSs. Through the assay, samples had been firstly hybridized in two steps after which positioned on a conjugate pad in a fashion that optimistic and adverse samples offered two and one pink traces, respectively, on the detection pad.
Outcomes:Â After PCR response with biotinylated primer, hybridization course of with particular MTB probe-dA70-100 toke 10 min, and working course of on the strip was carried out inside 5 min.
Conclusion:Â We confirmed that LFTS can discriminate a specific bacteria pressure from others. The LFTSs might be redesigned for detection of different pathogenic genomes.
Key phrases:Â Gold; Lateral Circulate; Metallic Nanoparticles; Nucleic Acid Hybridization; Take a look at Strip; Tuberculosis.
genpcr
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HCV Seroconversion Panel Donor# 63792 (6 X 1 mL) |
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HCV9046 |
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HCV9050 |
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HCV9054 |
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HCV9055 |
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) HCV Seroconversion Panel Donor# 66861 (11 X 1 mL) |
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HCV9057 |
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) HCV Seroconversion Panel Donor# 66647 (5 X 1 mL) |
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HCV9058 |
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HCV9094 |
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) HCV Seroconversion Panel Donor# 1038851 (5 X 1 mL) |
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HCV9095 |
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 Positive control tissue section for each antibody; Based on availability INQUIRE |
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Control-Slides |
Innovex |
Set of 5 |
EUR 176 |
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HCV10235 |
Zeptometrix |
5 X 1 mL |
EUR 512.8 |
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) Zika Virus Seroconversion Panel (7 X 0.5mL) |
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KZMC047 |
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EUR 2367.12 |
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) HIV Seroconversion Panel Donor# 65404 (7 X mL) |
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HIV9013 |
Zeptometrix |
7 X mL |
EUR 1548.64 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
) HCV Genotype Panel (14 X 0.5 mL) |
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KZMC049 |
Zeptometrix |
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EUR 2191.36 |
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) EBV Seroconversion Panel Donor# 283843421 (14 X 1 mL) |
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EBV9097 |
Zeptometrix |
14 X 1 mL |
EUR 1548.64 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
) HBV Seroconversion Panel Donor# 64090 (5 X 1 mL) |
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HBV10231 |
Zeptometrix |
5 X 1 mL |
EUR 582.48 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
) HBV Seroconversion Panel Donor# 64017 (6 X 1 mL) |
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HBV10232 |
Zeptometrix |
6 X 1 mL |
EUR 582.48 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
) HBV Seroconversion Panel Donor# 65449 (9 X 1 mL) |
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HBV11000 |
Zeptometrix |
9 X 1 mL |
EUR 859.12 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
) HBV Seroconversion Panel Donor# 65524 (8 X 1 mL) |
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HBV11001 |
Zeptometrix |
8 X 1 mL |
EUR 859.12 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
) HBV Seroconversion Panel Donor# 65556 (6 X 1 mL) |
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HBV11002 |
Zeptometrix |
6 X 1 mL |
EUR 859.12 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
) HBV Seroconversion Panel Donor# 65584 (8 X 1 mL) |
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HBV11003 |
Zeptometrix |
8 X 1 mL |
EUR 859.12 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
) HBV Seroconversion Panel Donor# 65732 (8 X 1 mL) |
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HBV11004 |
Zeptometrix |
8 X 1 mL |
EUR 859.12 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
) HBV Seroconversion Panel Donor# 65777 (14 X 1 mL) |
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HBV11005 |
Zeptometrix |
14 X 1 mL |
EUR 859.12 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
) HBV Seroconversion Panel Donor# 66201 (17 X 1 mL) |
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HBV11006 |
Zeptometrix |
17 X 1 mL |
EUR 859.12 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
) HBV Seroconversion Panel Donor# 66537 (14 X 1 mL) |
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HBV11007 |
Zeptometrix |
14 X 1 mL |
EUR 859.12 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
) HBV Seroconversion Panel Donor# 67303 (18 X 1 mL) |
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HBV11008 |
Zeptometrix |
18 X 1 mL |
EUR 859.12 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
) HBV Seroconversion Panel Donor# 67449 (23 X 1 mL) |
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HBV11009 |
Zeptometrix |
23 X 1 mL |
EUR 859.12 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
) HBV Seroconversion Panel Donor# 67457 (18 X 1 mL) |
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HBV11010 |
Zeptometrix |
18 X 1 mL |
EUR 859.12 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
) HBV Seroconversion Panel Donor# 67694 (14 X 1 mL) |
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HBV11011 |
Zeptometrix |
14 X 1 mL |
EUR 859.12 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
) HBV Seroconversion Panel Donor# 67773 (6 X 1 mL) |
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HBV11012 |
Zeptometrix |
6 X 1 mL |
EUR 859.12 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
) HBV Seroconversion Panel Donor# 67962 (35 X 1 mL) |
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HBV11013 |
Zeptometrix |
35 X 1 mL |
EUR 859.12 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
Growth and Validation of a Actual-Time PCR Primarily based Assay to Detect Adulteration with Corn in Business Turmeric Powder Merchandise
Turmeric, or Curcuma longa, is usually consumed within the South East Asian nations as a medical product and as meals on account of its therapeutic properties. Nevertheless, with growing demand for turmeric powder, adulterated turmeric powders combined with different low cost starch powders, equivalent to from corn or cassava, are being distributed by meals suppliers for financial profit.
Right here, we developed molecular markers utilizing quantitative real-time PCR to establish adulteration in industrial turmeric powder merchandise. Chloroplast genes, equivalent to matK, atpF, and ycf2, had been used to design species-specific primers for C. longa and Zea mays. Of the six primer pairs designed and examined, the correlation coefficients (R2) had been larger than 0.99 and slopes had been -3.136 to -3.498.
The effectivity of the primers was between 93.14 and 108.4%. The specificity of the primers was confirmed with ten different species, which may very well be deliberately added to C. longa powders or used as elements in advanced turmeric meals. In whole, 20 blind samples and 10 industrialC. longa meals merchandise had been examined with the designed primer units to display the effectiveness of this method to detect the addition of Z. mays merchandise in turmeric powders. Taken collectively, the real-time PCR assay developed right here has the potential to contribute to meals security and the safety of shopper’s rights.
Key phrases:Â Curcuma longa; DNA markers; SYBR-GREEN real-time PCR; Zea mays; anti meals fraud; species identification
 Anti-Hu CD16 Alexa Fluor® 488 |
|
A4-646-T100 |
ExBio |
100 tests |
EUR 269 |
) Goat anti Mouse IgG1 (Alexa Fluor 488) |
|
43R-1649 |
Fitzgerald |
500 ug |
EUR 570 |
|
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488) |
 AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate] |
|
16891 |
AAT Bioquest |
1 mg |
EUR 176 |
|
|
 AF488 Phalloidin [equivalent to Alexa Fluor® 488 phalloidin] |
|
23153 |
AAT Bioquest |
300 Tests |
EUR 306 |
|
|
 Anti-Hu CD72 Alexa Fluor® 488 |
|
A4-310-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Bov CD9 Alexa Fluor® 488 |
|
A4-354-C100 |
ExBio |
0.1 mg |
EUR 269 |
 Endoglin/CD105 Alexa Fluor |
|
FC15024 |
Neuromics |
100 Tests |
EUR 448 |
 Alexa Fluor 488–conjugated) Goat Anti-Mouse IgG(H+L) Alexa Fluor 488–conjugated |
|
S0017 |
Affbiotech |
200ul |
EUR 304 |
 Alexa Fluor 488–conjugated) Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488–conjugated |
|
S0018 |
Affbiotech |
200ul |
EUR 304 |
, Alexa Fluor® 488 Conjugated) Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated |
|
Ab8032-001 |
GenDepot |
0.5mg |
EUR 435 |
 Alexa Fluor<sup>®</sup> 488) Anti-Hu CD3 zeta (pY153) Alexa Fluor® 488 |
|
A4-686-C100 |
ExBio |
0.1 mg |
EUR 269 |
 Alexa Fluor<sup>®</sup> 488) Anti-Hu CD3 zeta (pY72) Alexa Fluor® 488 |
|
A4-712-C100 |
ExBio |
0.1 mg |
EUR 269 |
 Alexa Fluor<sup>®</sup> 488) Anti-Hu CD3 zeta (pY142) Alexa Fluor® 488 |
|
A4-730-C100 |
ExBio |
0.1 mg |
EUR 269 |
 Alexa Fluor<sup>®</sup> 488) Anti-Hu CD3 zeta (pY111) Alexa Fluor® 488 |
|
A4-737-C100 |
ExBio |
0.1 mg |
EUR 269 |
) SAM FCM (Alexa Fluor 647) |
|
abx098902-100tests |
Abbexa |
100 tests |
EUR 1233 |
|
|
 Monoclonal Antibody (104G4) (Alexa Fluor<sup>®</sup> 488)) Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor® 488) |
|
M09406 |
BosterBio |
100ug |
EUR 565 |
|
Description: Mouse Monoclonal LAMP3 (human) Antibody (104G4) (Alexa Fluor® 488). Validated in IHC and tested in Human. |
 Alpha Fluor™ 488 Hydroxylamine |
|
1900 |
AAT Bioquest |
1 mg |
EUR 306 |
|
|
) Anti-LAMP3 antibody (Alexa-fluor 546) |
|
STJ170005 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface. |
) Anti-LAMP3 antibody (Alexa-fluor 647) |
|
STJ170006 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface. |
) Anti-IL3RA antibody (Alexa-fluor 546) |
|
STJ170010 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ‚Äö√†√∂‚à ö√¢‚Äö√¢√†‚Äö√†√® subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment |
) Anti-IL3RA antibody (Alexa-fluor 647) |
|
STJ170011 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ‚Äö√†√∂‚à ö√¢‚Äö√¢√†‚Äö√†√® subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment |
) Anti-CD207 antibody (Alexa-fluor 546) |
|
STJ170015 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307) |
) Anti-CD207 antibody (Alexa-fluor 647) |
|
STJ170016 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307) |
) Anti-IL7R antibody (Alexa-fluor 546) |
|
STJ170021 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, na‚Äö√†√∂‚à ö√¢¬¨¬®‚à ö√≤ve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54) |
) Anti-IL7R antibody (Alexa-fluor 647) |
|
STJ170022 |
St John's Laboratory |
100 µg |
EUR 393 |
|
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, na‚Äö√†√∂‚à ö√¢¬¨¬®‚à ö√≤ve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54) |
 Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor<sup>®</sup> 488)) Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor® 488) |
|
M02316 |
BosterBio |
100ug |
EUR 580 |
|
Description: Mouse Monoclonal Langerin (human) Antibody (DCGM4/122D5) (Alexa Fluor® 488). Validated in IHC and tested in Human. |
 conjugate) Streptavidin-Alexa488 (Alexas fluor 488) conjugate |
|
SV-A488-100 |
Alpha Diagnostics |
100 tests |
EUR 225 |
-Alexa 488 Fluor conjugate (adsorbed with human IgG)) Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG) |
|
50336 |
Alpha Diagnostics |
0.5 ml |
EUR 225 |
 Alpha Fluor™ 532 acid [equivalent to Alexa Fluor™ 532 acid] |
|
1795 |
AAT Bioquest |
10 mg |
EUR 393 |
|
|
) Mouse IgG1-Alexa 488 conjugate (isotype control) |
|
20102-101-A488 |
Alpha Diagnostics |
50 Tests |
EUR 263 |
 AF350-streptavidin conjugate [Streptavidin, Alexa Fluor™ 350 Conjugate] |
|
16890 |
AAT Bioquest |
1 mg |
EUR 176 |
|
|
 AF594-streptavidin conjugate [Streptavidin, Alexa Fluor™ 594 Conjugate] |
|
16892 |
AAT Bioquest |
1 mg |
EUR 176 |
|
|
 AF350 Phalloidin [equivalent to Alexa Fluor® 350 phalloidin] |
|
23150 |
AAT Bioquest |
300 Tests |
EUR 306 |
|
|
 AF594 Phalloidin [equivalent to Alexa Fluor® 594 phalloidin] |
|
23158 |
AAT Bioquest |
300 Tests |
EUR 306 |
|
|
 Anti-Hu CD30 Alexa Fluor® 700 |
|
A7-455-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD94 Alexa Fluor® 700 |
|
A7-727-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD56 Alexa Fluor® 700 |
|
A7-789-T100 |
ExBio |
100 tests |
EUR 269 |
 (Alexa Fluor 594)) Donkey anti Goat IgG (H + L) (Alexa Fluor 594) |
|
43R-ID005AF |
Fitzgerald |
500 ug |
EUR 338 |
|
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594) |
 (Alexa Fluor 594)) Donkey anti Rat IgG (H + L) (Alexa Fluor 594) |
|
43R-ID022AF |
Fitzgerald |
500 ug |
EUR 364 |
|
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594) |
 (Alexa Fluor 647)) Donkey anti Goat IgG (H + L) (Alexa Fluor 647) |
|
43R-ID028AF |
Fitzgerald |
500 ug |
EUR 430 |
|
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647) |
Donkey anti Rat IgG (H + L) (Alexa Fluor 594) |
|
43R-ID047AF |
Fitzgerald |
500 ug |
EUR 462 |
|
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594) |
 (Alexa Fluor 594)) Donkey anti Chicken IgY (H + L) (Alexa Fluor 594) |
|
43R-ID056AF |
Fitzgerald |
500 ug |
EUR 343 |
|
Description: Donkey anti Chicken IgY secondary antibody (H + L) (Alexa Fluor 594) |
 (Alexa Fluor 647)) Donkey anti Chicken IgY (H + L) (Alexa Fluor 647) |
|
43R-ID060AF |
Fitzgerald |
300 ug |
EUR 425 |
|
Description: Donkey anti Chicken IgY (H + L) (Fab'2) (Alexa Fluor 647) |
 (Alexa Fluor 594)) Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594) |
|
43R-IR016AF |
Fitzgerald |
1 mg |
EUR 281 |
|
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594) |
 Alexa Fluor 594–conjugated) Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated |
|
S0005 |
Affbiotech |
200ul |
EUR 376 |
 Alexa Fluor 594–conjugated) Goat Anti-Rabbit IgG(H+L) Alexa Fluor 594–conjugated |
|
S0006 |
Affbiotech |
200ul |
EUR 376 |
 Alexa Fluor 647–conjugated) Goat Anti-Rabbit IgG(H+L) Alexa Fluor 647–conjugated |
|
S0013 |
Affbiotech |
200ul |
EUR 304 |
 Alexa Fluor 647–conjugated) Goat Anti-Mouse IgG(H+L) Alexa Fluor 647–conjugated |
|
S0014 |
Affbiotech |
200ul |
EUR 304 |
 Mouse pre-microRNA Expression Construct mir-488 |
|
MMIR-488-PA-1 |
SBI |
Bacterial Streak |
EUR 684 |
|
|
, Alexa Fluor® 594 Conjugated) Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated |
|
Ab8011-001 |
GenDepot |
1mg |
EUR 334 |
 Alexa Fluor<sup>®</sup> 647) Anti-Hu CD3 zeta (pY153) Alexa Fluor® 647 |
|
A6-686-C100 |
ExBio |
0.1 mg |
EUR 269 |
 Alexa Fluor<sup>®</sup> 647) Anti-Hu CD3 zeta (pY72) Alexa Fluor® 647 |
|
A6-712-C100 |
ExBio |
0.1 mg |
EUR 269 |
 Alexa Fluor<sup>®</sup> 647) Anti-Hu CD3 zeta (pY142) Alexa Fluor® 647 |
|
A6-730-C100 |
ExBio |
0.1 mg |
EUR 269 |
 Alexa Fluor<sup>®</sup> 647) Anti-Hu CD3 zeta (pY111) Alexa Fluor® 647 |
|
A6-737-C100 |
ExBio |
0.1 mg |
EUR 269 |
 (Fab 2) (Alexa Fluor 594)) Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594) |
|
43R-ID012AF |
Fitzgerald |
300 ug |
EUR 410 |
|
Description: Donkey anti Goat IgG (H + L) secondary antibody (Fab'2) (Alexa Fluor 594) |
-Alexa 594 Fluor conjugate (adsorbed with human IgG)) Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG) |
|
50337 |
Alpha Diagnostics |
0.5 ml |
EUR 225 |
 Anti-Cytokeratins Alexa Fluor488 |
|
A4-108-C025 |
ExBio |
0.025 mg |
EUR 175 |
Anti-Cytokeratins Alexa Fluor488 |
|
A4-108-C100 |
ExBio |
0.1 mg |
EUR 310 |
 Anti-PSMA Alexa Fluor488 |
|
A4-539-C025 |
ExBio |
0.025 mg |
EUR 227 |
Anti-PSMA Alexa Fluor488 |
|
A4-539-C100 |
ExBio |
0.1 mg |
EUR 414 |
 Anti-FoxP3 Alexa Fluor488 |
|
A4-601-C025 |
ExBio |
0.025 mg |
EUR 201 |
Anti-FoxP3 Alexa Fluor488 |
|
A4-601-C100 |
ExBio |
0.1 mg |
EUR 362 |
 Anti-Phosphotyrosine Alexa Fluor647 |
|
A6-263-C025 |
ExBio |
0.025 mg |
EUR 154 |
Anti-Phosphotyrosine Alexa Fluor647 |
|
A6-263-C100 |
ExBio |
0.1 mg |
EUR 269 |
 Anti-LCK Alexa Fluor647 |
|
A6-269-C025 |
ExBio |
0.025 mg |
EUR 206 |
Anti-LCK Alexa Fluor647 |
|
A6-269-C100 |
ExBio |
0.1 mg |
EUR 373 |
 Anti-FoxP3 Alexa Fluor647 |
|
A6-601-C025 |
ExBio |
0.025 mg |
EUR 201 |
Anti-FoxP3 Alexa Fluor647 |
|
A6-601-C100 |
ExBio |
0.1 mg |
EUR 362 |
 Tide Fluor 2-LL-37 |
|
H-8286.0100 |
Bachem |
0.1mg |
EUR 312 |
|
Description: Sum Formula: C205H340N60O53+dye |
Tide Fluor 2-LL-37 |
|
H-8286.0500 |
Bachem |
0.5mg |
EUR 1017 |
|
Description: Sum Formula: C205H340N60O53+dye |
 Hepatitis C Virus (HCV) NS5 Genotype-2a ) Recombinant (E.Coli) Hepatitis C Virus (HCV) NS5 Genotype-2a |
|
RP-488 |
Alpha Diagnostics |
100 ug |
EUR 286 |
 4-1BB Ligand / TNFSF9 Recombinant Protein |
|
11-488 |
ProSci |
0.1 mg |
EUR 595.25 |
|
Description: Tumor necrosis factor ligand superfamily member 9 (4-1BBL) is also known as 4-1BB ligand, CD137L or TNFSF9, which is a cytokine that binds to TNFRSF9. 4-1BBL is the high affinity ligand of 4-1BB. 4-1BBL induces the proliferation of activated peripheral blood T-cells. Also, 4-1BBL may have a role in activation-induced cell death (AICD). Furthermore, 4-1BBL may play a role in cognate interactions between T-cells and B-cells/macrophages. As for diseases, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases. |
 IL-6R Recombinant Protein |
|
90-488 |
ProSci |
50 ug |
EUR 516.5 |
|
Description: Interleukin 6 receptor (IL-6R; CD126) is a type I cytokine receptor, which is a protein complex consisting of an IL-6 receptor subunit (IL-6R) and interleukin 6 signal transducer glycoprotein 130 (gp130). It is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. Dysregulated production of IL-6 and IL-6R are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. |
 Serpin F1 Recombinant Protein |
|
91-488 |
ProSci |
0.05 mg |
EUR 542.75 |
|
Description: Serpin F1 is a secreted glycoprotein that belongs to the noninhibitory serpin. It has an alpha/beta core serine-protease inhibitor domain, three major beta-sheets, and ten alpha-helices. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins. Serpin F1 is a new promising approach for the treatment of osteosarcoma and has been described as a natural angiogenesis inhibitor with neurotrophic and immune-modulation properties. The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Levels of the natural ocular anti-angiogenic factor SentrinF1 (PEDF) is associated with proliferative retinopathy. |
 HVEM Recombinant Protein |
|
92-488 |
ProSci |
0.05 mg |
EUR 311.75 |
|
Description: Mouse Protein Tnfrsf14, is a type I transmembrane protein belonging to the TNF receptor superfamily. It is tumor necrosis factor receptor superfamily member 14 and expressed on the surface of T cells during the resting state. Interaction of HVEM with TNF family member LIGHT co-stimulates T cells and promotes inflammation. HVEM also triggers inhibitory signaling cascade in effector T (Teff) cells and regulatory T cells (Tregs) as a ligand of B and T lymphocyte attenuator. Tnfrsf14 is detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. It has demonstrated that HVEM Ig is able to exert a significant antiviral effect against HSV-1 infection in vivo. |
) Monoclonal Anti-Monkey IgG-Alexa 488 Conj. (specific for monkey; no reactivity with human or animals IgG) |
|
70030-AF488 |
Alpha Diagnostics |
50 tests |
EUR 347 |
 CellBriteâ„¢ Fix 488 |
|
30090 |
Biotium |
1KIT |
EUR 644 |
|
Description: Minimum order quantity: 1 unit of 1KIT |
 Anti-Cytokeratin 18 Alexa Fluor488 |
|
A4-106-C025 |
ExBio |
0.025 mg |
EUR 186 |
Anti-Cytokeratin 18 Alexa Fluor488 |
|
A4-106-C100 |
ExBio |
0.1 mg |
EUR 331 |
 Anti-Cytokeratin 19 Alexa Fluor488 |
|
A4-120-C025 |
ExBio |
0.025 mg |
EUR 186 |
Anti-Cytokeratin 19 Alexa Fluor488 |
|
A4-120-C100 |
ExBio |
0.1 mg |
EUR 331 |
 Anti-Ki-67 Alexa Fluor488 |
|
A4-155-T025 |
ExBio |
25 tests |
EUR 154 |
Anti-Ki-67 Alexa Fluor488 |
|
A4-155-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD45 Alexa Fluor488 |
|
A4-160-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD193 Alexa Fluor488 |
|
A4-161-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD279 Alexa Fluor488 |
|
A4-176-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD43 Alexa Fluor488 |
|
A4-220-T025 |
ExBio |
25 tests |
EUR 154 |
Anti-Hu CD43 Alexa Fluor488 |
|
A4-220-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD44 Alexa Fluor488 |
|
A4-221-T025 |
ExBio |
25 tests |
EUR 154 |
Anti-Hu CD44 Alexa Fluor488 |
|
A4-221-T100 |
ExBio |
100 tests |
EUR 269 |
Anti-Hu CD45 Alexa Fluor488 |
|
A4-222-T025 |
ExBio |
25 tests |
EUR 154 |
Anti-Hu CD45 Alexa Fluor488 |
|
A4-222-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD55 Alexa Fluor488 |
|
A4-230-T025 |
ExBio |
25 tests |
EUR 154 |
Anti-Hu CD55 Alexa Fluor488 |
|
A4-230-T100 |
ExBio |
100 tests |
EUR 269 |
 Anti-Hu CD50 Alexa Fluor488 |
|
A4-266-T025 |
ExBio |
25 tests |
EUR 154 |
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