Real-Time PCR Quantification Methods in the Identification of Poultry Species in Meat Products

Comparability of Actual-Time PCR Quantification Strategies within the Identification of Poultry Species in Meat Merchandise

Poultry meat is consumed worldwide and is liable to meals fraud due to massive value variations amongst meat from totally different poultry species. Exact and delicate analytical strategies are needed to regulate poultry meat merchandise. We selected species-specific sequences of the cytochrome b gene to develop two multiplex real-time polymerase chain response (real-time PCR) techniques: one for hen (Gallus gallus), guinea fowl (Numida meleagris), and pheasant (Phasianus colchicus), and one for quail (Coturnix japonica) and turkey (Meleagris gallopavo).

For every species, added meat may very well be detected all the way down to 0.5 % w/w. No cross reactions had been seen. For these two real-time PCR techniques, we utilized three totally different quantification strategies: (A) with relative commonplace curves, (B) with matrix-specific multiplication elements, and (C) with an inner DNA reference sequence to normalize and to regulate inhibition. All three quantification strategies had affordable restoration charges from 43% to 173%. Methodology B had extra accepted restoration charges, i.e., within the vary 70-130%, particularly 83% in comparison with 75% for technique A or C.

Key phrases: hen; guinea fowl; pheasant; quail; quantification; real-time PCR; turkey

Lateral Circulate Genochromatographic Strip for Bare-Eye Detection of Mycobacterium Tuberculosis PCR Merchandise with Gold Nanoparticles as a Reporter

Background: Mycobacterium tuberculosis (MTB) is a pathogen inflicting tuberculosis (TB) in human, and TB may cause huge social and financial disruptions. Lateral circulate take a look at strips (LFTSs) are cheap, moveable, disposable, fast, and easy-to-use analytical instruments.

Goal: LFTSs had been ready for the detection of MTB. LFTSs had been fabricated utilizing a brand new particular probe for MTB H37Rv, primarily based on IS6110 sequence gene, and tailed with poly deoxyadenine (dA).

Materials and strategies: On this experimental research, to create take a look at and management zones, streptavidin (STP) and a 150-mer dA had been dotted on a nitrocellolose membrane. Gold nanoparticles (GNPs) had been conjugated with poly deoxythymidine sequence and positioned on the conjugate pad.

The composition of immersion buffers for pattern pad and conjugate pad, working answer, options of GNPs-S-dT150 and STP had been launched. DNA genome of MTB and Mycobacterium bovisin scientific samples was amplified with PCR, after which detected by the LFTSs. Through the assay, samples had been firstly hybridized in two steps after which positioned on a conjugate pad in a fashion that optimistic and adverse samples offered two and one pink traces, respectively, on the detection pad.

Outcomes: After PCR response with biotinylated primer, hybridization course of with particular MTB probe-dA70-100 toke 10 min, and working course of on the strip was carried out inside 5 min.

Conclusion: We confirmed that LFTS can discriminate a specific bacteria pressure from others. The LFTSs might be redesigned for detection of different pathogenic genomes.

Key phrases: Gold; Lateral Circulate; Metallic Nanoparticles; Nucleic Acid Hybridization; Take a look at Strip; Tuberculosis.

genpcr

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Growth and Validation of a Actual-Time PCR Primarily based Assay to Detect Adulteration with Corn in Business Turmeric Powder Merchandise

Turmeric, or Curcuma longa, is usually consumed within the South East Asian nations as a medical product and as meals on account of its therapeutic properties. Nevertheless, with growing demand for turmeric powder, adulterated turmeric powders combined with different low cost starch powders, equivalent to from corn or cassava, are being distributed by meals suppliers for financial profit.

Right here, we developed molecular markers utilizing quantitative real-time PCR to establish adulteration in industrial turmeric powder merchandise. Chloroplast genes, equivalent to matK, atpF, and ycf2, had been used to design species-specific primers for C. longa and Zea mays. Of the six primer pairs designed and examined, the correlation coefficients (R²) had been larger than 0.99 and slopes had been -3.136 to -3.498.

The effectivity of the primers was between 93.14 and 108.4%. The specificity of the primers was confirmed with ten different species, which may very well be deliberately added to C. longa powders or used as elements in advanced turmeric meals. In whole, 20 blind samples and 10 industrialC. longa meals merchandise had been examined with the designed primer units to display the effectiveness of this method to detect the addition of Z. mays merchandise in turmeric powders. Taken collectively, the real-time PCR assay developed right here has the potential to contribute to meals security and the safety of shopper’s rights.

Key phrases: Curcuma longa; DNA markers; SYBR-GREEN real-time PCR; Zea mays; anti meals fraud; species identification

Anti-LAMP3 antibody (Alexa-fluor 488)
STJ170004	St John's Laboratory	100 µg	EUR 393.00
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-IL3RA antibody (Alexa-fluor 488)
STJ170009	St John's Laboratory	100 µg	EUR 393.00
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ‚Äö√†√∂‚àö√¢‚Äö√¢√†‚Äö√†√® subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-CD207 antibody (Alexa-fluor 488)
STJ170014	St John's Laboratory	100 µg	EUR 393.00
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-IL7R antibody (Alexa-fluor 488)
STJ170020	St John's Laboratory	100 µg	EUR 393.00
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, na‚Äö√†√∂‚àö√¢¬¨¬®‚àö√≤ve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-Hu CD16 Alexa Fluor® 488
A4-646-T100	ExBio	100 tests	EUR 269.00

Goat anti Mouse IgG1 (Alexa Fluor 488)
43R-1649	Fitzgerald	500 ug	EUR 570.00
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488)

Anti-Hu CD72 Alexa Fluor^® 488
A4-310-T100	ExBio	100 tests	EUR 269.00

Anti-Bov CD9 Alexa Fluor^® 488
A4-354-C100	ExBio	0.1 mg	EUR 269.00

AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate]
16891	AAT Bioquest	1 mg	EUR 176.00
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12171501

AF488 Phalloidin [equivalent to Alexa Fluor® 488 phalloidin]
23153	AAT Bioquest	300 Tests	EUR 306.00
R-phrase: R23, R24, R25 H-Phrase: H301, H311, H331 Symbol for dangerous compounds: T UNSPEC Code: 12352200

Endoglin/CD105 Alexa Fluor
FC15024	Neuromics	100 Tests	EUR 448.00

Goat Anti-Mouse IgG(H+L) Alexa Fluor 488–conjugated
S0017	Affbiotech	200ul	EUR 304.00

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488–conjugated
S0018	Affbiotech	200ul	EUR 304.00

Anti-Hu CD3 zeta (pY153) Alexa Fluor^® 488
A4-686-C100	ExBio	0.1 mg	EUR 269.00

Anti-Hu CD3 zeta (pY72) Alexa Fluor^® 488
A4-712-C100	ExBio	0.1 mg	EUR 269.00

Anti-Hu CD3 zeta (pY142) Alexa Fluor^® 488
A4-730-C100	ExBio	0.1 mg	EUR 269.00

Anti-Hu CD3 zeta (pY111) Alexa Fluor^® 488
A4-737-C100	ExBio	0.1 mg	EUR 269.00

Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated
Ab8032-001	GenDepot	0.5mg	EUR 435.00

SAM FCM (Alexa Fluor 647)
abx098902-100tests	Abbexa	100 tests	EUR 1233.00
Shipped within 5-10 working days.

Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor^® 488)
M09406	BosterBio	100ug	EUR 565.00
Description: Mouse Monoclonal LAMP3 (human) Antibody (104G4) (Alexa Fluor^® 488). Validated in IHC and tested in Human.

Alpha Fluor™ 488 amine
1705	AAT Bioquest	1 mg	EUR 306.00
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12171501

Alpha Fluor™ 488 Hydroxylamine
1900	AAT Bioquest	1 mg	EUR 306.00
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12171501

Anti-LAMP3 antibody (Alexa-fluor 546)
STJ170005	St John's Laboratory	100 µg	EUR 393.00
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-LAMP3 antibody (Alexa-fluor 647)
STJ170006	St John's Laboratory	100 µg	EUR 393.00
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-IL3RA antibody (Alexa-fluor 546)
STJ170010	St John's Laboratory	100 µg	EUR 393.00
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ‚Äö√†√∂‚àö√¢‚Äö√¢√†‚Äö√†√® subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-IL3RA antibody (Alexa-fluor 647)
STJ170011	St John's Laboratory	100 µg	EUR 393.00
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ‚Äö√†√∂‚àö√¢‚Äö√¢√†‚Äö√†√® subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-CD207 antibody (Alexa-fluor 546)
STJ170015	St John's Laboratory	100 µg	EUR 393.00
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-CD207 antibody (Alexa-fluor 647)
STJ170016	St John's Laboratory	100 µg	EUR 393.00
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-IL7R antibody (Alexa-fluor 546)
STJ170021	St John's Laboratory	100 µg	EUR 393.00
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, na‚Äö√†√∂‚àö√¢¬¨¬®‚àö√≤ve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-IL7R antibody (Alexa-fluor 647)
STJ170022	St John's Laboratory	100 µg	EUR 393.00
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, na‚Äö√†√∂‚àö√¢¬¨¬®‚àö√≤ve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor^® 488)
M02316	BosterBio	100ug	EUR 580.00
Description: Mouse Monoclonal Langerin (human) Antibody (DCGM4/122D5) (Alexa Fluor^® 488). Validated in IHC and tested in Human.

Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG)
50336	Alpha Diagnostics	0.5 ml	EUR 225.00

Alpha Fluor™ 488 NHS Ester
1812	AAT Bioquest	1 mg	EUR 219.00
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12171501

Streptavidin-Alexa488 (Alexas fluor 488) conjugate
SV-A488-100	Alpha Diagnostics	100 tests	EUR 225.00

Alpha Fluor™ 532 acid [equivalent to Alexa Fluor™ 532 acid]
1795	AAT Bioquest	10 mg	EUR 393.00
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12171501

Mouse IgG1-Alexa 488 conjugate (isotype control)
20102-101-A488	Alpha Diagnostics	50 Tests	EUR 263.00

Anti-Hu CD30 Alexa Fluor^® 700
A7-455-T100	ExBio	100 tests	EUR 269.00

Anti-Hu CD94 Alexa Fluor^® 700
A7-727-T100	ExBio	100 tests	EUR 269.00

Anti-Hu CD56 Alexa Fluor^® 700
A7-789-T100	ExBio	100 tests	EUR 269.00

AF350-streptavidin conjugate [Streptavidin, Alexa Fluor™ 350 Conjugate]
16890	AAT Bioquest	1 mg	EUR 176.00
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12171501

AF594-streptavidin conjugate [Streptavidin, Alexa Fluor™ 594 Conjugate]
16892	AAT Bioquest	1 mg	EUR 176.00
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12171501

AF350 Phalloidin [equivalent to Alexa Fluor® 350 phalloidin]
23150	AAT Bioquest	300 Tests	EUR 306.00
R-phrase: R23, R24, R25 H-Phrase: H301, H311, H331 Symbol for dangerous compounds: T UNSPEC Code: 12352200

AF594 Phalloidin [equivalent to Alexa Fluor® 594 phalloidin]
23158	AAT Bioquest	300 Tests	EUR 306.00
R-phrase: R23, R24, R25 H-Phrase: H301, H311, H331 Symbol for dangerous compounds: T UNSPEC Code: 12352200

Donkey anti Goat IgG (H + L) (Alexa Fluor 594)
43R-ID005AF	Fitzgerald	500 ug	EUR 338.00
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)
43R-ID022AF	Fitzgerald	500 ug	EUR 364.00
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Goat IgG (H + L) (Alexa Fluor 647)
43R-ID028AF	Fitzgerald	500 ug	EUR 430.00
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)
43R-ID047AF	Fitzgerald	500 ug	EUR 462.00
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 594)
43R-ID056AF	Fitzgerald	500 ug	EUR 343.00
Description: Donkey anti Chicken IgY secondary antibody (H + L) (Alexa Fluor 594)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 647)
43R-ID060AF	Fitzgerald	300 ug	EUR 425.00
Description: Donkey anti Chicken IgY (H + L) (Fab'2) (Alexa Fluor 647)

Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594)
43R-IR016AF	Fitzgerald	1 mg	EUR 281.00
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594)

Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated
S0005	Affbiotech	200ul	EUR 376.00

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 594–conjugated
S0006	Affbiotech	200ul	EUR 376.00

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 647–conjugated
S0013	Affbiotech	200ul	EUR 304.00

Goat Anti-Mouse IgG(H+L) Alexa Fluor 647–conjugated
S0014	Affbiotech	200ul	EUR 304.00

Mouse pre-microRNA Expression Construct mir-488
MMIR-488-PA-1	SBI	Bacterial Streak	EUR 684.00
Category: MicroRNA Tools

Anti-Hu CD3 zeta (pY153) Alexa Fluor^® 647
A6-686-C100	ExBio	0.1 mg	EUR 269.00

Anti-Hu CD3 zeta (pY72) Alexa Fluor^® 647
A6-712-C100	ExBio	0.1 mg	EUR 269.00

Anti-Hu CD3 zeta (pY142) Alexa Fluor^® 647
A6-730-C100	ExBio	0.1 mg	EUR 269.00

Anti-Hu CD3 zeta (pY111) Alexa Fluor^® 647
A6-737-C100	ExBio	0.1 mg	EUR 269.00

Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated
Ab8011-001	GenDepot	1mg	EUR 334.00

Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594)
43R-ID012AF	Fitzgerald	300 ug	EUR 410.00
Description: Donkey anti Goat IgG (H + L) secondary antibody (Fab'2) (Alexa Fluor 594)

Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG)
50337	Alpha Diagnostics	0.5 ml	EUR 225.00

Recombinant (E.Coli) Hepatitis C Virus (HCV) NS5 Genotype-2a
RP-488	Alpha Diagnostics	100 ug	EUR 286.00

Anti-Cytokeratins Alexa Fluor488
A4-108-C025	ExBio	0.025 mg	EUR 175.00

Anti-Cytokeratins Alexa Fluor488
A4-108-C100	ExBio	0.1 mg	EUR 310.00

Anti-PSMA Alexa Fluor488
A4-539-C025	ExBio	0.025 mg	EUR 227.00

Anti-PSMA Alexa Fluor488
A4-539-C100	ExBio	0.1 mg	EUR 414.00

Anti-FoxP3 Alexa Fluor488
A4-601-C025	ExBio	0.025 mg	EUR 201.00

Anti-FoxP3 Alexa Fluor488
A4-601-C100	ExBio	0.1 mg	EUR 362.00

Anti-Phosphotyrosine Alexa Fluor647
A6-263-C025	ExBio	0.025 mg	EUR 154.00

Anti-Phosphotyrosine Alexa Fluor647
A6-263-C100	ExBio	0.1 mg	EUR 269.00

Anti-LCK Alexa Fluor647
A6-269-C025	ExBio	0.025 mg	EUR 206.00

Anti-LCK Alexa Fluor647
A6-269-C100	ExBio	0.1 mg	EUR 373.00

Anti-FoxP3 Alexa Fluor647
A6-601-C025	ExBio	0.025 mg	EUR 201.00

Anti-FoxP3 Alexa Fluor647
A6-601-C100	ExBio	0.1 mg	EUR 362.00

Tide Fluor 2-LL-37
H-8286.0100	Bachem	0.1mg	EUR 312.00
Description: Sum Formula: C205H340N60O53+dye

Tide Fluor 2-LL-37
H-8286.0500	Bachem	0.5mg	EUR 1017.00
Description: Sum Formula: C205H340N60O53+dye

Monoclonal Anti-Monkey IgG-Alexa 488 Conj. (specific for monkey; no reactivity with human or animals IgG)
70030-AF488	Alpha Diagnostics	50 tests	EUR 347.00

CellBriteâ„¢ Fix 488
30090	Biotium	1KIT	EUR 644.00
Description: Minimum order quantity: 1 unit of 1KIT

Anti-Cytokeratin 18 Alexa Fluor488
A4-106-C025	ExBio	0.025 mg	EUR 186.00

Anti-Cytokeratin 18 Alexa Fluor488
A4-106-C100	ExBio	0.1 mg	EUR 331.00

Anti-Cytokeratin 19 Alexa Fluor488
A4-120-C025	ExBio	0.025 mg	EUR 186.00

Anti-Cytokeratin 19 Alexa Fluor488
A4-120-C100	ExBio	0.1 mg	EUR 331.00

Anti-Ki-67 Alexa Fluor488
A4-155-T025	ExBio	25 tests	EUR 154.00

Anti-Ki-67 Alexa Fluor488
A4-155-T100	ExBio	100 tests	EUR 269.00

Anti-Hu CD45 Alexa Fluor488
A4-160-T100	ExBio	100 tests	EUR 269.00

Anti-Hu CD193 Alexa Fluor488
A4-161-T100	ExBio	100 tests	EUR 269.00

Anti-Hu CD279 Alexa Fluor488
A4-176-T100	ExBio	100 tests	EUR 269.00

Anti-Hu CD43 Alexa Fluor488
A4-220-T025	ExBio	25 tests	EUR 154.00

Anti-Hu CD43 Alexa Fluor488
A4-220-T100	ExBio	100 tests	EUR 269.00

Anti-Hu CD44 Alexa Fluor488
A4-221-T025	ExBio	25 tests	EUR 154.00

Anti-Hu CD44 Alexa Fluor488
A4-221-T100	ExBio	100 tests	EUR 269.00

Anti-Hu CD45 Alexa Fluor488
A4-222-T025	ExBio	25 tests	EUR 154.00

Anti-Hu CD45 Alexa Fluor488
A4-222-T100	ExBio	100 tests	EUR 269.00

Anti-Hu CD55 Alexa Fluor488
A4-230-T025	ExBio	25 tests	EUR 154.00

Anti-Hu CD55 Alexa Fluor488
A4-230-T100	ExBio	100 tests	EUR 269.00

Anti-Hu CD50 Alexa Fluor488
A4-266-T025	ExBio	25 tests	EUR 154.00

Comparability of Actual-Time PCR Quantification Strategies within the Identification of Poultry Species in Meat Merchandise

Lateral Circulate Genochromatographic Strip for Bare-Eye Detection of Mycobacterium Tuberculosis PCR Merchandise with Gold Nanoparticles as a Reporter

Growth and Validation of a Actual-Time PCR Primarily based Assay to Detect Adulteration with Corn in Business Turmeric Powder Merchandise

Anti-LAMP3 antibody (Alexa-fluor 488)

Anti-IL3RA antibody (Alexa-fluor 488)

Anti-CD207 antibody (Alexa-fluor 488)

Anti-IL7R antibody (Alexa-fluor 488)

Anti-Hu CD16 Alexa Fluor® 488

Goat anti Mouse IgG1 (Alexa Fluor 488)

Anti-Hu CD72 Alexa Fluor® 488

Anti-Bov CD9 Alexa Fluor® 488

AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate]

AF488 Phalloidin [equivalent to Alexa Fluor® 488 phalloidin]

Endoglin/CD105 Alexa Fluor

Goat Anti-Mouse IgG(H+L) Alexa Fluor 488–conjugated

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488–conjugated

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 488

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 488

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 488

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 488

Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated

SAM FCM (Alexa Fluor 647)

Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor® 488)

Alpha Fluor™ 488 amine

Alpha Fluor™ 488 Hydroxylamine

Anti-LAMP3 antibody (Alexa-fluor 546)

Anti-LAMP3 antibody (Alexa-fluor 647)

Anti-IL3RA antibody (Alexa-fluor 546)

Anti-IL3RA antibody (Alexa-fluor 647)

Anti-CD207 antibody (Alexa-fluor 546)

Anti-CD207 antibody (Alexa-fluor 647)

Anti-IL7R antibody (Alexa-fluor 546)

Anti-IL7R antibody (Alexa-fluor 647)

Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor® 488)

Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG)

Alpha Fluor™ 488 NHS Ester

Streptavidin-Alexa488 (Alexas fluor 488) conjugate

Alpha Fluor™ 532 acid [equivalent to Alexa Fluor™ 532 acid]

Mouse IgG1-Alexa 488 conjugate (isotype control)

Anti-Hu CD30 Alexa Fluor® 700

Anti-Hu CD94 Alexa Fluor® 700

Anti-Hu CD56 Alexa Fluor® 700

AF350-streptavidin conjugate [Streptavidin, Alexa Fluor™ 350 Conjugate]

AF594-streptavidin conjugate [Streptavidin, Alexa Fluor™ 594 Conjugate]

AF350 Phalloidin [equivalent to Alexa Fluor® 350 phalloidin]

AF594 Phalloidin [equivalent to Alexa Fluor® 594 phalloidin]

Donkey anti Goat IgG (H + L) (Alexa Fluor 594)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

Donkey anti Goat IgG (H + L) (Alexa Fluor 647)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 594)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 647)

Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594)

Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 594–conjugated

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 647–conjugated

Goat Anti-Mouse IgG(H+L) Alexa Fluor 647–conjugated

Mouse pre-microRNA Expression Construct mir-488

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 647

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 647

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 647

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 647

Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated

Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594)

Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG)

Recombinant (E.Coli) Hepatitis C Virus (HCV) NS5 Genotype-2a

Anti-Cytokeratins Alexa Fluor488

Anti-Cytokeratins Alexa Fluor488

Anti-PSMA Alexa Fluor488

Anti-PSMA Alexa Fluor488

Anti-FoxP3 Alexa Fluor488

Anti-FoxP3 Alexa Fluor488

Anti-Phosphotyrosine Alexa Fluor647

Anti-Phosphotyrosine Alexa Fluor647

Anti-LCK Alexa Fluor647

Anti-LCK Alexa Fluor647

Anti-FoxP3 Alexa Fluor647

Anti-FoxP3 Alexa Fluor647

Tide Fluor 2-LL-37

Tide Fluor 2-LL-37

Anti-Hu CD72 Alexa Fluor^® 488

Anti-Bov CD9 Alexa Fluor^® 488

Anti-Hu CD3 zeta (pY153) Alexa Fluor^® 488

Anti-Hu CD3 zeta (pY72) Alexa Fluor^® 488

Anti-Hu CD3 zeta (pY142) Alexa Fluor^® 488

Anti-Hu CD3 zeta (pY111) Alexa Fluor^® 488

Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor^® 488)

Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor^® 488)

Anti-Hu CD30 Alexa Fluor^® 700

Anti-Hu CD94 Alexa Fluor^® 700

Anti-Hu CD56 Alexa Fluor^® 700

Anti-Hu CD3 zeta (pY153) Alexa Fluor^® 647

Anti-Hu CD3 zeta (pY72) Alexa Fluor^® 647

Anti-Hu CD3 zeta (pY142) Alexa Fluor^® 647

Anti-Hu CD3 zeta (pY111) Alexa Fluor^® 647