Excessive-efficiency transformation of archaea by direct PCR merchandise with its utility to directed evolution of a thermostable enzyme
Hyperthermophilic archaea with distinctive biochemical and physiological traits are necessary organisms for basic analysis of life science and have nice potential for biotechnological functions. Nonetheless, low transformation effectivity of international DNA molecules impedes developments in genetic modification instruments and industrial functions.
- On this research, we utilized extended overlap extension PCR (POE-PCR) to generate multimeric DNA molecules after which remodeled them into two hyperthermophilic archaea, Thermococcus kodakarensis KOD1 and Pyrococcus yayanosii A1.
- This research was the primary instance to display the improved transformation efficiencies of POE-PCR merchandise by an element of roughly 100 for T. kodakarensis KOD1 and eight for P. yayanosii A1, respectively, relative to round shuttle plasmids.
- Moreover, directed evolution of a modestly thermophilic enzyme, Methanothermococcus okinawensis 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), was carried out to acquire extra steady ones on account of excessive transformation effectivity of T. kodakarensis (i.e. ~3 × 104 CFU per μg DNA). T. kodakarensis harbouring essentially the most thermostable MoHMGR mutant can develop within the presence of a thermostable antibiotic simvastatin at 85°C and even greater temperatures.
- This excessive transformation effectivity method couldn’t solely assist develop extra hyperthermophilic enzyme mutants by way of directed evolution but in addition simplify genetical modification of archaea, which could possibly be novel hosts for industrial biotechnology
Screening of business meat merchandise from grocery store chains for feline derivatives utilizing SP-PCR-RLFP and lab-on-a-chip
Willpower of feline meat in meals merchandise is a vital situation for social, well being, financial and spiritual concern. Therefore this paper documented the applying of species particular polymerase chain reaction-restriction fragment size polymorphism (SP-PCR-RFLP) assay concentrating on a short-fragments (69 bp) of mitochondrial cytochrome b (cytb) gene to display feline meat in industrial meat merchandise utilizing lab-on-a-chip.
The SP-PCR assay proved its specificity theoretically and experimentally whereas testing with totally different widespread animal, aquatic and plant species of DNA. The feline particular (69 bp, 43- and 26-bp) attribute molecular
DNA sample was noticed by SP-PCR and RFLP evaluation.
For assay efficiency, it was examined in three several types of industrial dummy meat merchandise equivalent to frankfurters, nuggets and meatballs and digested with AluI-restriction enzyme. The best sensitivity of the assay utilizing lab-on-a-chip was as little as 0.1 pg or 0.01 % (w/w) in industrial dummy meat merchandise. Now we have additionally utilized this assay to display three necessary industrial meat merchandise of six totally different model from six grocery store chains positioned at three totally different states of Malaysia.
Thus whole 378 samples had been examined to validate the specificity, sensitivity, stability of the assay and utilization of it for industrial meat product screening.
Key phrases: Industrial meat merchandise; Feline meat; Halal; Lab-on-a-chip; Restriction digestion.

genpcr
Alexa Fluor® 594 anti-mouse CD31 |
E16FMJ031-100U |
EnoGene |
100 μg |
EUR 931.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 594 anti-mouse CD45 |
E16FMJ045-100U |
EnoGene |
100 μg |
EUR 931.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 594 anti-mouse CD68 |
E16FMJ068-100U |
EnoGene |
100 μg |
EUR 1365 |
Description: Available in various conjugation types. |
Alexa Fluor® 594 anti-human CD8a |
E16FHJ008a-100U |
EnoGene |
100 μg |
EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 594 anti-human CD14 |
E16FHJ0142-100U |
EnoGene |
100 μg |
EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 594 anti-human CD19 |
E16FHJ019-100U |
EnoGene |
100 μg |
EUR 1083.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 594 anti-mouse CD11c |
E16FMJ011c-100U |
EnoGene |
100 μg |
EUR 758.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 594 anti-mouse CD106 |
E16FMJ106-100U |
EnoGene |
100 μg |
EUR 931.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 594 anti-human CD11a |
E16FHJ011a-100U |
EnoGene |
100 μg |
EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 594 anti-human CD11b |
E16FHJ011b-100U |
EnoGene |
100 μg |
EUR 931.67 |
Description: Available in various conjugation types. |
Mouse Anti-Human IgA - Alexa Fluor 594 |
DL87387A-100ul |
DL Develop |
100 ul |
EUR 190 |
|
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Human IgA - Alexa Fluor 594 |
DL87387A-500ul |
DL Develop |
500 ul |
EUR 280 |
|
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Human IgM - Alexa Fluor 594 |
DL87390A-100ul |
DL Develop |
100 ul |
EUR 155 |
|
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Human IgM - Alexa Fluor 594 |
DL87390A-500ul |
DL Develop |
500 ul |
EUR 350 |
|
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Human IgA - Alexa Fluor 594 |
E38A3634 |
EnoGene |
100μL |
EUR 125 |
Mouse Anti-Human IgM - Alexa Fluor 594 |
E38A3638 |
EnoGene |
100μL |
EUR 125 |
Mouse Anti-Human IgM - Alexa Fluor 594 |
YLF0361-100ul |
Shanghai YL Biotech |
100 ul |
EUR 130 |
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Human IgM - Alexa Fluor 594 |
YLF0361-1ml |
Shanghai YL Biotech |
1 ml |
EUR 480 |
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Human IgM - Alexa Fluor 594 |
YLF0361-500ul |
Shanghai YL Biotech |
500 ul |
EUR 290 |
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Human IgA - Alexa Fluor 594 |
YLF0564-100ul |
Shanghai YL Biotech |
100 ul |
EUR 130 |
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Human IgA - Alexa Fluor 594 |
YLF0564-1ml |
Shanghai YL Biotech |
1 ml |
EUR 300 |
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Human IgA - Alexa Fluor 594 |
YLF0564-500ul |
Shanghai YL Biotech |
500 ul |
EUR 210 |
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Rabbit Anti-Human IgM - Alexa Fluor 594 |
DL87379A-100ul |
DL Develop |
100 ul |
EUR 190 |
|
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Rabbit Anti-Human IgM - Alexa Fluor 594 |
DL87379A-500ul |
DL Develop |
500 ul |
EUR 280 |
|
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Rabbit Anti-Human IgM - Alexa Fluor 594 |
E38A3625 |
EnoGene |
100μL |
EUR 125 |
Rabbit Anti-Human IgM - Alexa Fluor 594 |
YLF0561-100ul |
Shanghai YL Biotech |
100 ul |
EUR 130 |
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Rabbit Anti-Human IgM - Alexa Fluor 594 |
YLF0561-1ml |
Shanghai YL Biotech |
1 ml |
EUR 300 |
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Rabbit Anti-Human IgM - Alexa Fluor 594 |
YLF0561-500ul |
Shanghai YL Biotech |
500 ul |
EUR 210 |
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Goat Anti-Human IgG Fc - Alexa Fluor 594 |
DL87358A-100ul |
DL Develop |
100 ul |
EUR 155 |
|
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Goat Anti-Human IgG Fc - Alexa Fluor 594 |
DL87358A-500ul |
DL Develop |
500 ul |
EUR 350 |
|
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Goat Anti-Human IgG Fc - Alexa Fluor 594 |
YLF0339-100ul |
Shanghai YL Biotech |
100 ul |
EUR 130 |
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Goat Anti-Human IgG Fc - Alexa Fluor 594 |
YLF0339-1ml |
Shanghai YL Biotech |
1 ml |
EUR 480 |
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Goat Anti-Human IgG Fc - Alexa Fluor 594 |
YLF0339-500ul |
Shanghai YL Biotech |
500 ul |
EUR 290 |
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Goat Anti-Human IgG Fab - Alexa Fluor 594 |
DL87357A-100ul |
DL Develop |
100 ul |
EUR 190 |
|
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Goat Anti-Human IgG Fab - Alexa Fluor 594 |
DL87357A-500ul |
DL Develop |
500 ul |
EUR 280 |
|
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Human IgG Fc - Alexa Fluor 594 |
DL87389A-100ul |
DL Develop |
100 ul |
EUR 190 |
|
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Human IgG Fc - Alexa Fluor 594 |
DL87389A-500ul |
DL Develop |
500 ul |
EUR 280 |
|
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Alexa Fluor® 594 anti-mouse CD206 (MMR) |
E16FMJ206-100U |
EnoGene |
100 μg |
EUR 1191.67 |
Description: Available in various conjugation types. |
Goat Anti-Human IgG Fab - Alexa Fluor 594 |
E38A3603 |
EnoGene |
100μL |
EUR 125 |
Mouse Anti-Human IgG Fc - Alexa Fluor 594 |
E38A3636 |
EnoGene |
100μL |
EUR 125 |
Goat Anti-Human IgG Fab - Alexa Fluor 594 |
YLF0558-100ul |
Shanghai YL Biotech |
100 ul |
EUR 130 |
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Goat Anti-Human IgG Fab - Alexa Fluor 594 |
YLF0558-1ml |
Shanghai YL Biotech |
1 ml |
EUR 300 |
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Goat Anti-Human IgG Fab - Alexa Fluor 594 |
YLF0558-500ul |
Shanghai YL Biotech |
500 ul |
EUR 210 |
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Human IgG Fc - Alexa Fluor 594 |
YLF0566-100ul |
Shanghai YL Biotech |
100 ul |
EUR 130 |
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Human IgG Fc - Alexa Fluor 594 |
YLF0566-1ml |
Shanghai YL Biotech |
1 ml |
EUR 300 |
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Human IgG Fc - Alexa Fluor 594 |
YLF0566-500ul |
Shanghai YL Biotech |
500 ul |
EUR 210 |
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Rabbit Anti-Human IgG Fc - Alexa Fluor 594 |
DL87376A-100ul |
DL Develop |
100 ul |
EUR 155 |
|
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Rabbit Anti-Human IgG Fc - Alexa Fluor 594 |
DL87376A-500ul |
DL Develop |
500 ul |
EUR 350 |
|
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Human IgG Fab - Alexa Fluor 594 |
DL87388A-100ul |
DL Develop |
100 ul |
EUR 190 |
|
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Human IgG Fab - Alexa Fluor 594 |
DL87388A-500ul |
DL Develop |
500 ul |
EUR 280 |
|
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Alexa Fluor® 594 anti-human/mouse CD44 |
E16FMJ044-100U |
EnoGene |
100 μg |
EUR 931.67 |
Description: Available in various conjugation types. |
Rabbit Anti-Human IgG Fc - Alexa Fluor 594 |
E38A3622 |
EnoGene |
100μL |
EUR 125 |
Mouse Anti-Human IgG Fab - Alexa Fluor 594 |
E38A3635 |
EnoGene |
100μL |
EUR 125 |
Rabbit Anti-Human IgG Fc - Alexa Fluor 594 |
YLF0354-100ul |
Shanghai YL Biotech |
100 ul |
EUR 130 |
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Rabbit Anti-Human IgG Fc - Alexa Fluor 594 |
YLF0354-1ml |
Shanghai YL Biotech |
1 ml |
EUR 480 |
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Rabbit Anti-Human IgG Fc - Alexa Fluor 594 |
YLF0354-500ul |
Shanghai YL Biotech |
500 ul |
EUR 290 |
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Human IgG Fab - Alexa Fluor 594 |
YLF0565-100ul |
Shanghai YL Biotech |
100 ul |
EUR 130 |
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Human IgG Fab - Alexa Fluor 594 |
YLF0565-1ml |
Shanghai YL Biotech |
1 ml |
EUR 300 |
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Human IgG Fab - Alexa Fluor 594 |
YLF0565-500ul |
Shanghai YL Biotech |
500 ul |
EUR 210 |
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Alexa Fluor® 594 anti-human/mouse CD11b |
E16FMJ011b-100U |
EnoGene |
100 μg |
EUR 931.67 |
Description: Available in various conjugation types. |
Donkey anti Rat IgG (H + L) (Alexa Fluor 594) |
43R-ID022AF |
Fitzgerald |
500 ug |
EUR 334 |
|
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594) |
Donkey anti Rat IgG (H + L) (Alexa Fluor 594) |
43R-ID047AF |
Fitzgerald |
500 ug |
EUR 465 |
|
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594) |
Goat Anti-Pig IgG (H&L) - Alexa Fluor 594 |
DL87364A-100ul |
DL Develop |
100 ul |
EUR 155 |
|
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Goat Anti-Pig IgG (H&L) - Alexa Fluor 594 |
DL87364A-500ul |
DL Develop |
500 ul |
EUR 350 |
|
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Goat Anti-Pig IgG (H&L) - Alexa Fluor 594 |
E38A3610 |
EnoGene |
100μL |
EUR 125 |
Goat Anti-Pig IgG (H&L) - Alexa Fluor 594 |
YLF0343-100ul |
Shanghai YL Biotech |
100 ul |
EUR 130 |
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Goat Anti-Pig IgG (H&L) - Alexa Fluor 594 |
YLF0343-1ml |
Shanghai YL Biotech |
1 ml |
EUR 480 |
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Goat Anti-Pig IgG (H&L) - Alexa Fluor 594 |
YLF0343-500ul |
Shanghai YL Biotech |
500 ul |
EUR 290 |
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Donkey anti Goat IgG (H + L) (Alexa Fluor 594) |
43R-ID005AF |
Fitzgerald |
500 ug |
EUR 333 |
|
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594) |
Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594 |
DL87393A-100ul |
DL Develop |
100 ul |
EUR 190 |
|
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594 |
DL87393A-500ul |
DL Develop |
500 ul |
EUR 280 |
|
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594 |
E38A3641 |
EnoGene |
100μL |
EUR 125 |
Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594 |
YLF0569-100ul |
Shanghai YL Biotech |
100 ul |
EUR 130 |
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594 |
YLF0569-1ml |
Shanghai YL Biotech |
1 ml |
EUR 300 |
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594 |
YLF0569-500ul |
Shanghai YL Biotech |
500 ul |
EUR 210 |
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads. |
Goat Anti-Human IgG (H&L) - Alexa Fluor 594 |
DL87356A-100ul |
DL Develop |
100 ul |
EUR 140 |
|
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Goat Anti-Human IgG (H&L) - Alexa Fluor 594 |
DL87356A-500ul |
DL Develop |
500 ul |
EUR 330 |
|
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. |
Multiplex PCR for simultaneous identification of the most typical European Opisthorchiid and Heterophyid in fish or fish merchandise
Amongst others, the households Opisthorchiidae and Heterophyidae contains a number of genera inflicting fish-borne zoonoses and distributed additionally in European Nations and which can be included within the ParaFishControl (Superior Instruments and Analysis Methods for Parasite Management in European farmed fish) H2020 EU venture.
Because of the small dimension of the metacercariae, the infective stage for human, these parasites can’t be detected visually in fish and monitoring requires professional utility of time-consuming methods. The goal of this was to develop a fast and inexpensive molecular technique primarily based on multiplex PCR for simultaneous identification of metacercariae of the most typical European Opisthorchiid and Heterophyid in fish or fish merchandise.
Key phrases: Europe; Fish merchandise; Freshwater fish; Heterophyidae; Multiplex PCR; Opisthorchiidae
TaqMan probe primarily based multiplex quantitative PCR assay for dedication of bovine, porcine and fish DNA in gelatin admixture, meals merchandise and dietary dietary supplements.
Detection of animal supplies in gelatin-based merchandise is required to deal with spiritual and cultural considerations, as a result of porcine and bovine gelatins are prohibited in Halal, Kosher and Hindus shopper goods. On this paper, multiplex quantitative polymerase chain response (qPCR) assay utilizing TaqMan probe was developed to discriminate bovine, porcine and fish gelatin species in a single assay platform.
The assay was particular to cattle, pigs and fish, having been examined in opposition to 14 non-target species. The restrict of detection, underneath gelatin admixed circumstances, was 0.005 ng/µL. Lastly, a pilot survey was undertaken testing 35 Halal branded processed meals and dietary gadgets. Out of 35 samples, solely two had been discovered to be optimistic for porcine species. The authenticity of those two qPCR merchandise was confirmed by DNA sequencing evaluation, which confirmed 99-100% similarity with Sus scrofa (Wild boar) species.
HDV Real Time RT-PCR Kit |
GWB-LRB030 |
GenWay Biotech |
25 tests |
Ask for price |
HCMV Real Time PCR Kit |
GWB-LRB009 |
GenWay Biotech |
25 tests |
Ask for price |
HCMV Real Time PCR Kit |
GWB-LRB010 |
GenWay Biotech |
25 tests |
Ask for price |
HEV IV Real Time RT-PCR Kit |
GWB-LRB031 |
GenWay Biotech |
25 tests |
Ask for price |
HEV IV Real Time RT-PCR Kit |
GWB-LRB032 |
GenWay Biotech |
25 tests |
Ask for price |
ONE STEP RT-PCR REAL TIME KIT |
MBT128-10R |
EWC Diagnostics |
1 unit |
EUR 37.4 |
Description: ONE STEP RT-PCR REAL TIME KIT |
ONE STEP RT-PCR REAL TIME KIT |
MBT128-25R |
EWC Diagnostics |
1 unit |
EUR 89.72 |
Description: ONE STEP RT-PCR REAL TIME KIT |
ONE STEP RT-PCR REAL TIME KIT |
MBT128-2X50R |
EWC Diagnostics |
1 unit |
EUR 314.51 |
Description: ONE STEP RT-PCR REAL TIME KIT |
ONE STEP RT-PCR REAL TIME KIT |
MBT128-50R |
EWC Diagnostics |
1 unit |
EUR 165.56 |
Description: ONE STEP RT-PCR REAL TIME KIT |
HCV Genetype Real Time RT-PCR Kit |
GWB-LRB027 |
GenWay Biotech |
25 tests |
Ask for price |
HCV Genetype Real Time RT-PCR Kit |
GWB-LRB028 |
GenWay Biotech |
25 tests |
Ask for price |
RV Real Time RT-PCR Detection kit |
TRI-L02M1 |
TRI Biotech |
32T |
EUR 705.6 |
RV Real Time RT-PCR Detection kit |
TRI-L02S1 |
TRI Biotech |
16T |
EUR 378 |
Measles Virus Real Time RT-PCR Kit |
GWB-LRB043 |
GenWay Biotech |
25 tests |
Ask for price |
Measles Virus Real Time RT-PCR Kit |
GWB-LRB044 |
GenWay Biotech |
25 tests |
Ask for price |
Rubella Virus Real Time RT-PCR Kit |
GWB-LRB045 |
GenWay Biotech |
25 tests |
Ask for price |
Rubella Virus Real Time RT-PCR Kit |
GWB-LRB046 |
GenWay Biotech |
25 tests |
Ask for price |
FCV Real Time RT-PCR Detection kit |
TRI-L14M1 |
TRI Biotech |
32T |
EUR 705.6 |
FCV Real Time RT-PCR Detection kit |
TRI-L14S1 |
TRI Biotech |
16T |
EUR 378 |
FIV Real Time RT-PCR Detection kit |
TRI-L22M1 |
TRI Biotech |
32T |
EUR 705.6 |
FIV Real Time RT-PCR Detection kit |
TRI-L22S1 |
TRI Biotech |
16T |
EUR 378 |
FIPV Real Time RT-PCR Detection kit |
TRI-L03M1 |
TRI Biotech |
32T |
EUR 453.6 |
FIPV Real Time RT-PCR Detection kit |
TRI-L03S1 |
TRI Biotech |
16T |
EUR 252 |
CPIV Real Time RT-PCR Detection kit |
TRI-L07M1 |
TRI Biotech |
32T |
EUR 453.6 |
CPIV Real Time RT-PCR Detection kit |
TRI-L07S1 |
TRI Biotech |
16T |
EUR 252 |
CSFV Real Time RT-PCR Detection kit |
TRI-L28M1 |
TRI Biotech |
48T |
EUR 1008 |
CSFV Real Time RT-PCR Detection kit |
TRI-L28S1 |
TRI Biotech |
24T |
EUR 529.2 |
PEDV Real Time RT-PCR Detection kit |
TRI-L34M1 |
TRI Biotech |
48T |
EUR 1008 |
PEDV Real Time RT-PCR Detection kit |
TRI-L34S1 |
TRI Biotech |
24T |
EUR 529.2 |
CP&MP Real Time PCR Kit |
GWB-LRB005 |
GenWay Biotech |
25 tests |
Ask for price |
CP&MP Real Time PCR Kit |
GWB-LRB006 |
GenWay Biotech |
25 tests |
Ask for price |
Chikungunya Virus Real Time RT-PCR Kit |
GWB-LRB001 |
GenWay Biotech |
25 tests |
Ask for price |
Chikungunya Virus Real Time RT-PCR Kit |
GWB-LRB002 |
GenWay Biotech |
25 tests |
Ask for price |
Monkeypox Virus Real Time PCR Kit |
PDPS-AR064 |
Creative Biogene |
1 unit |
Ask for price |
Description: Creative Biogene Monkeypox Virus Real Time PCR Kit is used for the detection of monkeypox Virus in serum or lesion exudate samples by using real time PCR systems. Monkeypox virus (MPV) is a double-stranded DNA, zoonotic virus and a species of the genus Orthopoxvirus in the family Poxviridae. It is one of the human orthopoxviruses that includes variola (VARV), cowpox (CPX), and vaccinia (VACV) viruses. The kit contains a specific ready-to-use system for the detection of the monkeypox Virus. Fluorescence is emitted and measured by the real time systems' optical unit during the PCR. |
VetAlert Johnes Real Time PCR Kit |
TC-9828-100 |
Tetracore |
100 rxns |
EUR 460 |
Monkeypox Virus Real Time PCR Kit |
ZD-0076-01 |
Gentaur Genprice |
25 tests/kit |
Ask for price |
|
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load. |
Monkeypox Virus Real Time PCR Kit |
ZD-0076-02 |
Gentaur Genprice |
25 tests/kit |
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Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load. |
Monkeypox Virus Real Time PCR Kit |
YJC70115NW-25T |
Jiangsu Bioperfectus Technologies |
25 tests/kit |
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Description: The Bioperfectus Monkeypox Virus Real Time PCR Kit is an in vitro diagnostic test, based on real-time PCR technology, for the detection of DNA from the Monkeypox virus. Specimens can be obtained from human serum, lesion exudate samples and scab. BSL-2 facilities with standard BSL-2 work practices may be used for the test of t he Monkeypox virus. |
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