Transformation of Archaea by Direct PCR Merchandise with its Utility To Directed Evolution

Excessive-efficiency transformation of archaea by direct PCR merchandise with its utility to directed evolution of a thermostable enzyme

Hyperthermophilic archaea with distinctive biochemical and physiological traits are necessary organisms for basic analysis of life science and have nice potential for biotechnological functions. Nonetheless, low transformation effectivity of international DNA molecules impedes developments in genetic modification instruments and industrial functions.

 

  • On this research, we utilized extended overlap extension PCR (POE-PCR) to generate multimeric DNA molecules after which remodeled them into two hyperthermophilic archaea, Thermococcus kodakarensis KOD1 and Pyrococcus yayanosii A1.
  • This research was the primary instance to display the improved transformation efficiencies of POE-PCR merchandise by an element of roughly 100 for T. kodakarensis KOD1 and eight for P. yayanosii A1, respectively, relative to round shuttle plasmids.
  • Moreover, directed evolution of a modestly thermophilic enzyme, Methanothermococcus okinawensis 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), was carried out to acquire extra steady ones on account of excessive transformation effectivity of T. kodakarensis (i.e. ~3 × 104 CFU per μg DNA). T. kodakarensis harbouring essentially the most thermostable MoHMGR mutant can develop within the presence of a thermostable antibiotic simvastatin at 85°C and even greater temperatures.
  • This excessive transformation effectivity method couldn’t solely assist develop extra hyperthermophilic enzyme mutants by way of directed evolution but in addition simplify genetical modification of archaea, which could possibly be novel hosts for industrial biotechnology

Screening of business meat merchandise from grocery store chains for feline derivatives utilizing SP-PCR-RLFP and lab-on-a-chip

Willpower of feline meat in meals merchandise is a vital situation for social, well being, financial and spiritual concern. Therefore this paper documented the applying of species particular polymerase chain reaction-restriction fragment size polymorphism (SP-PCR-RFLP) assay concentrating on a short-fragments (69 bp) of mitochondrial cytochrome b (cytb) gene to display feline meat in industrial meat merchandise utilizing lab-on-a-chip.
The SP-PCR assay proved its specificity theoretically and experimentally whereas testing with totally different widespread animal, aquatic and plant species of DNA. The feline particular (69 bp, 43- and 26-bp) attribute molecular DNA sample was noticed by SP-PCR and RFLP evaluation.

For assay efficiency, it was examined in three several types of industrial dummy meat merchandise equivalent to frankfurters, nuggets and meatballs and digested with AluI-restriction enzyme. The best sensitivity of the assay utilizing lab-on-a-chip was as little as 0.1 pg or 0.01 % (w/w) in industrial dummy meat merchandise. Now we have additionally utilized this assay to display three necessary industrial meat merchandise of six totally different model from six grocery store chains positioned at three totally different states of Malaysia.

Thus whole 378 samples had been examined to validate the specificity, sensitivity, stability of the assay and utilization of it for industrial meat product screening.
Key phrases: Industrial meat merchandise; Feline meat; Halal; Lab-on-a-chip; Restriction digestion.
genpcr

genpcr

Alexa Fluor® 594 anti-mouse CD45

E16FMJ045-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-mouse CD68

E16FMJ068-100U 100 μg
EUR 1365
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-human CD8a

E16FHJ008a-100U 100 μg
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-human CD14

E16FHJ0142-100U 100 μg
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-human CD19

E16FHJ019-100U 100 μg
EUR 1083.33
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-mouse CD11c

E16FMJ011c-100U 100 μg
EUR 758.33
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-mouse CD106

E16FMJ106-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-human CD11a

E16FHJ011a-100U 100 μg
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-human CD11b

E16FHJ011b-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Mouse Anti-Human IgA - Alexa Fluor 594

DL87387A-100ul 100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgA - Alexa Fluor 594

DL87387A-500ul 500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 594

DL87390A-100ul 100 ul
EUR 155
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 594

DL87390A-500ul 500 ul
EUR 350
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgA - Alexa Fluor 594

E38A3634 100μL
EUR 125

Mouse Anti-Human IgM - Alexa Fluor 594

E38A3638 100μL
EUR 125

Mouse Anti-Human IgA - Alexa Fluor 594

YLF0564-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgA - Alexa Fluor 594

YLF0564-1ml 1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgA - Alexa Fluor 594

YLF0564-500ul 500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 594

YLF0361-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 594

YLF0361-1ml 1 ml
EUR 480
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 594

YLF0361-500ul 500 ul
EUR 290
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 594

DL87379A-100ul 100 ul
EUR 190
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 594

DL87379A-500ul 500 ul
EUR 280
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 594

E38A3625 100μL
EUR 125

Rabbit Anti-Human IgM - Alexa Fluor 594

YLF0561-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 594

YLF0561-1ml 1 ml
EUR 300
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 594

YLF0561-500ul 500 ul
EUR 210
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594

DL87358A-100ul 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594

DL87358A-500ul 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594

E38A3604 100ug/100ul
EUR 125
Description: Available in various conjugation types.

Goat Anti-Human IgG Fc - Alexa Fluor 594

YLF0339-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594

YLF0339-1ml 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594

YLF0339-500ul 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 594

DL87357A-100ul 100 ul
EUR 190
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 594

DL87357A-500ul 500 ul
EUR 280
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 594

DL87389A-100ul 100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 594

DL87389A-500ul 500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Alexa Fluor® 594 anti-mouse CD206 (MMR)

E16FMJ206-100U 100 μg
EUR 1191.67
Description: Available in various conjugation types.

Goat Anti-Human IgG Fab - Alexa Fluor 594

E38A3603 100μL
EUR 125

Mouse Anti-Human IgG Fc - Alexa Fluor 594

E38A3636 100μL
EUR 125

Goat Anti-Human IgG Fab - Alexa Fluor 594

YLF0558-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 594

YLF0558-1ml 1 ml
EUR 300
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 594

YLF0558-500ul 500 ul
EUR 210
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 594

YLF0566-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 594

YLF0566-1ml 1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 594

YLF0566-500ul 500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

DL87376A-100ul 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

DL87376A-500ul 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fab - Alexa Fluor 594

DL87388A-100ul 100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fab - Alexa Fluor 594

DL87388A-500ul 500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Alexa Fluor® 594 anti-human/mouse CD44

E16FMJ044-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

E38A3622 100μL
EUR 125

Mouse Anti-Human IgG Fab - Alexa Fluor 594

E38A3635 100μL
EUR 125

Mouse Anti-Human IgG Fab - Alexa Fluor 594

YLF0565-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fab - Alexa Fluor 594

YLF0565-1ml 1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fab - Alexa Fluor 594

YLF0565-500ul 500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

YLF0354-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

YLF0354-1ml 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

YLF0354-500ul 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Alexa Fluor® 594 anti-human/mouse CD11b

E16FMJ011b-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

DL87364A-100ul 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

DL87364A-500ul 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

E38A3610 100μL
EUR 125

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

43R-ID022AF 500 ug
EUR 334
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

43R-ID047AF 500 ug
EUR 465
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0343-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0343-1ml 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0343-500ul 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594

DL87393A-100ul 100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594

DL87393A-500ul 500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594

E38A3641 100μL
EUR 125

Donkey anti Goat IgG (H + L) (Alexa Fluor 594)

43R-ID005AF 500 ug
EUR 333
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594)

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0569-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0569-1ml 1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0569-500ul 500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG (H&L) - Alexa Fluor 594

DL87356A-100ul 100 ul
EUR 140
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG (H&L) - Alexa Fluor 594

DL87356A-500ul 500 ul
EUR 330
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Multiplex PCR for simultaneous identification of the most typical European Opisthorchiid and Heterophyid in fish or fish merchandise

Amongst others, the households Opisthorchiidae and Heterophyidae contains a number of genera inflicting fish-borne zoonoses and distributed additionally in European Nations and which can be included within the ParaFishControl (Superior Instruments and Analysis Methods for Parasite Management in European farmed fish) H2020 EU venture.
Because of the small dimension of the metacercariae, the infective stage for human, these parasites can’t be detected visually in fish and monitoring requires professional utility of time-consuming methods. The goal of this was to develop a fast and inexpensive molecular technique primarily based on multiplex PCR for simultaneous identification of metacercariae of the most typical European Opisthorchiid and Heterophyid in fish or fish merchandise.
Key phrases: Europe; Fish merchandise; Freshwater fish; Heterophyidae; Multiplex PCR; Opisthorchiidae
TaqMan probe primarily based multiplex quantitative PCR assay for dedication of bovine, porcine and fish DNA in gelatin admixture, meals merchandise and dietary dietary supplements.
Detection of animal supplies in gelatin-based merchandise is required to deal with spiritual and cultural considerations, as a result of porcine and bovine gelatins are prohibited in Halal, Kosher and Hindus shopper goods. On this paper, multiplex quantitative polymerase chain response (qPCR) assay utilizing TaqMan probe was developed to discriminate bovine, porcine and fish gelatin species in a single assay platform.
The assay was particular to cattle, pigs and fish, having been examined in opposition to 14 non-target species. The restrict of detection, underneath gelatin admixed circumstances, was 0.005 ng/µL. Lastly, a pilot survey was undertaken testing 35 Halal branded processed meals and dietary gadgets. Out of 35 samples, solely two had been discovered to be optimistic for porcine species. The authenticity of those two qPCR merchandise was confirmed by DNA sequencing evaluation, which confirmed 99-100% similarity with Sus scrofa (Wild boar) species.

HCMV Real Time PCR Kit

GWB-LRB010 25 tests Ask for price

Human Lung Cancer PCR Primer Library

HLUCPL-I 1 set
EUR 657.6

Human Schwann Cell PCR Primer Library

HSCH-I 1 set
EUR 657.6

HEV IV Real Time RT-PCR Kit

GWB-LRB031 25 tests Ask for price

HEV IV Real Time RT-PCR Kit

GWB-LRB032 25 tests Ask for price

ONE STEP RT-PCR REAL TIME KIT

MBT128-10R 1 unit
EUR 37.4
Description: ONE STEP RT-PCR REAL TIME KIT

ONE STEP RT-PCR REAL TIME KIT

MBT128-25R 1 unit
EUR 89.72
Description: ONE STEP RT-PCR REAL TIME KIT

ONE STEP RT-PCR REAL TIME KIT

MBT128-2X50R 1 unit
EUR 314.51
Description: ONE STEP RT-PCR REAL TIME KIT

ONE STEP RT-PCR REAL TIME KIT

MBT128-50R 1 unit
EUR 165.56
Description: ONE STEP RT-PCR REAL TIME KIT

CP&MP Real Time PCR Kit

GWB-LRB005 25 tests Ask for price

CP&MP Real Time PCR Kit

GWB-LRB006 25 tests Ask for price

HCV Genetype Real Time RT-PCR Kit

GWB-LRB027 25 tests Ask for price

HCV Genetype Real Time RT-PCR Kit

GWB-LRB028 25 tests Ask for price

Measles Virus Real Time RT-PCR Kit

GWB-LRB043 25 tests Ask for price

Measles Virus Real Time RT-PCR Kit

GWB-LRB044 25 tests Ask for price

Rubella Virus Real Time RT-PCR Kit

GWB-LRB045 25 tests Ask for price

Rubella Virus Real Time RT-PCR Kit

GWB-LRB046 25 tests Ask for price

Chikungunya Virus Real Time RT-PCR Kit

GWB-LRB001 25 tests Ask for price

Chikungunya Virus Real Time RT-PCR Kit

GWB-LRB002 25 tests Ask for price

Monkeypox Virus Real Time PCR Kit

PDPS-AR064 1 unit Ask for price
Description: Creative Biogene Monkeypox Virus Real Time PCR Kit is used for the detection of monkeypox Virus in serum or lesion exudate samples by using real time PCR systems. Monkeypox virus (MPV) is a double-stranded DNA, zoonotic virus and a species of the genus Orthopoxvirus in the family Poxviridae. It is one of the human orthopoxviruses that includes variola (VARV), cowpox (CPX), and vaccinia (VACV) viruses. The kit contains a specific ready-to-use system for the detection of the monkeypox Virus. Fluorescence is emitted and measured by the real time systems' optical unit during the PCR.

Monkeypox Virus Real Time PCR Kit

ZD-0076-01 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit

ZD-0076-02 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit

YJC70115NW-25T 25 tests/kit Ask for price
Description: The Bioperfectus Monkeypox Virus Real Time PCR Kit is an in vitro diagnostic test, based on real-time PCR technology, for the detection of DNA from the Monkeypox virus. Specimens can be obtained from human serum, lesion exudate samples and scab. BSL-2 facilities with standard BSL-2 work practices may be used for the test of t he Monkeypox virus.

VetAlert Johnes Real Time PCR Kit

TC-9828-100 100 rxns
EUR 460

HBV Quantitative Real Time PCR Kit

GWB-LRB013 25 tests Ask for price

HBV Quantitative Real Time PCR Kit

GWB-LRB014 25 tests Ask for price

Toxoplasma Gondii Real Time PCR Kit

GWB-LRB049 25 tests Ask for price

Toxoplasma Gondii Real Time PCR Kit

GWB-LRB050 25 tests Ask for price

HEV I & III Real Time RT-PCR Kit

GWB-LRB033 25 tests Ask for price

HEV I & III Real Time RT-PCR Kit

GWB-LRB034 25 tests Ask for price

HEV General Type Real Time RT-PCR Kit

GWB-LRB037 25 tests Ask for price

HEV General Type Real Time RT-PCR Kit

GWB-LRB038 25 tests Ask for price

AzuraQuant cDNA Synthesis Kit - 100 Reactions

AZ-1996 100 Reactions
EUR 446.4

Chlamydia Pheumoniae (CP) Real Time PCR Kit

GWB-LRB003 25 tests Ask for price

Chlamydia Pheumoniae (CP) Real Time PCR Kit

GWB-LRB004 25 tests Ask for price

AzuraQuant cDNA Synthesis Kit - 25 Reactions

AZ-1995 25 Reactions
EUR 183.6

BP/MP/CP Multiplex Real Time PCR Kit

GWB-LRB007 25 tests Ask for price

BP/MP/CP Multiplex Real Time PCR Kit

GWB-LRB008 25 tests Ask for price

HBV Quantitative & YMDD Mutation Real Time PCR Kit

GWB-LRB015 25 tests Ask for price

HBV Quantitative & YMDD Mutation Real Time PCR Kit

GWB-LRB016 25 tests Ask for price

HBV Quantitative & YMDD Mutation Real Time PCR Kit

GWB-LRB017 25 tests Ask for price

HBV Quantitative & YMDD Mutation Real Time PCR Kit

GWB-LRB018 25 tests Ask for price

HEV I, II, III, & IV Real Time RT-PCR Kit

GWB-LRB035 25 tests Ask for price

HEV I, II, III, & IV Real Time RT-PCR Kit

GWB-LRB036 25 tests Ask for price

HBV Genetype B,C&D Real Time PCR Kit

GWB-LRB023 25 tests Ask for price

HBV Genetype B,C&D Real Time PCR Kit

GWB-LRB024 25 tests Ask for price

HBV Adefovir-resistant Mutants Real Time PCR Kit

GWB-LRB019 25 tests Ask for price

HBV Adefovir-resistant Mutants Real Time PCR Kit

GWB-LRB020 25 tests Ask for price

Transfusion-transmitted Virus (TTV) Real Time PCR Kit

GWB-LRB041 25 tests Ask for price

Transfusion-transmitted Virus (TTV) Real Time PCR Kit

GWB-LRB042 25 tests Ask for price

HBV Precore Site 1896 Mutation Real Time PCR Kit

GWB-LRB021 25 tests Ask for price

HBV Precore Site 1896 Mutation Real Time PCR Kit

GWB-LRB022 25 tests Ask for price

genesig Real time PCR universal meat detection kit

Z-Path-SS-uni-meat 100 tests
EUR 602
Description: Universal meat

Measles Virus and Rubella Virus Real Time RT-PCR Kit

GWB-LRB047 25 tests Ask for price

Measles Virus and Rubella Virus Real Time RT-PCR Kit

GWB-LRB048 25 tests Ask for price

genesig Real time PCR Species specific kit, A.platyrhynchos

Z-Path-SS-A.platyrhynchos 100 tests
EUR 602
Description: A.platyrhynchos

ASF 3.0 Dry Real‐Time PCR with IC

TC-9113-064 each
EUR 800

genesig Real time PCR Species specific kit for C.hircus

Z-Path-SS-C.hircus 100 tests
EUR 602
Description: C.hircus

genesig Real time PCR Species specific kit for E.asinus

Z-Path-SS-E.asinus 100 tests
EUR 602
Description: E.asinus

genesig Real time PCR Species specific kit for G.gallus

Z-Path-SS-G.gallus 100 tests
EUR 602
Description: G.gallus

genesig Real time PCR Adv Species specific kit, G.gallus

Z-Path-SS-G.gallus-Adv 100 tests
EUR 808
Description: G.gallus

genesig Real time PCR Species specific kit for B.bubalis

Z-Path-SS-B.bubalis 100 tests
EUR 602
Description: B.bubalis

genesig Real time PCR Species specific kit for Venison

Z-Path-SS-Venison 100 tests
EUR 602
Description: Venison

genesig Real time PCR Species specific kit for E.caballus

Z-Path-SS-E.caballus 100 tests
EUR 602
Description: E.caballus

genesig Real time PCR Adv Species specific kit, E.caballus

Z-Path-SS-E.caballus-Adv 100 tests
EUR 808
Description: E.caballus

genesig Real time PCR Species specific kit for O.anatinus

Z-Path-SS-O.anatinus 100 tests
EUR 602
Description: O.anatinus

0.2ML CLEAR STRIP CAPS FOR REAL TIME PCR

PCR-2CP-RT-C 125/pk
EUR 267.6
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

genesig Real time PCR Adv Species specific kit, A.platyrhyn

Z-Path-SS-A.platyrhyncos-Adv 100 tests
EUR 808
Description: A.platyrhynchos

genesig Real time PCR Species specific kit for M.gallopavo

Z-Path-SS-M.gallopavo 100 tests
EUR 602
Description: M.gallopavo

genesig Real time PCR Adv Species specific kit, M.gallopavo

Z-Path-SS-M.gallopavo-Adv 100 tests
EUR 808
Description: M.gallopavo

genesig Real time PCR Species specific kit for P.africanus

Z-Path-SS-P.africanus 100 tests
EUR 602
Description: P.africanus

genesig Real time PCR Species specific kit for A.graveolens

Z-Path-SS-A.graveolens 100 tests
EUR 602
Description: A.graveolens

genesig Real time PCR Species specific kit for C.familiaris

Z-Path-SS-C.familiaris 100 tests
EUR 602
Description: C.familiaris

genesig Real time PCR Adv Species specific kit,C.familiaris

Z-Path-SS-C.familiaris-Adv 100 tests
EUR 808
Description: C.familiaris

ASF 3.0 Dry Real‐Time PCR Dilution Buffer

TC-9114-064 each
EUR 90

Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit

RR-0478-02 25 tests/kit
EUR 1189.2
Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems.

genesig Real time PCR Species specific kit for Bos taurus

Z-Path-SS-B.taurus 100 tests
EUR 602
Description: B.taurus

genesig Real time PCR Adv Species specific kit, Bos taurus

Z-Path-SS-B.taurus-Adv 100 tests
EUR 808
Description: B.taurus

genesig Real time PCR Adv Species specific kit for C.hircus

Z-Path-SS-C.hircus-Adv 100 tests
EUR 808
Description: C.hircus

genesig Real time PCR Adv Species specific kit for E.asinus

Z-Path-SS-E.asinus-Adv 100 tests
EUR 808
Description: E.asinus

genesig Real time PCR Species specific kit for Ovis aries

Z-Path-SS-O.aries 100 tests
EUR 602
Description: O.aries

genesig Real time PCR Adv Species specific kit, Ovis aries

Z-Path-SS-O.aries-Adv 100 tests
EUR 808
Description: O.aries

genesig Real time PCR Species specific kit for Sus scrofa

Z-Path-SS-S.scrofa 100 tests
EUR 602
Description: S.scrofa

genesig Real time PCR Adv Species specific kit, Sus scrofa

Z-Path-SS-S.scrofa-Adv 100 tests
EUR 808
Description: S.scrofa

genesig Real time PCR Adv species specific kit for B.bubalis

Z-Path-SS-B.bubalis-Adv 100 tests
EUR 808
Description: B.bubalis

genesig Real time PCR Species specific kit for Universal fis

Z-Path-SS-uni-fish-V2.0 100 tests
EUR 602
Description: Universal fish

Azura PureView 1 Kb DNA Ladder - 100 Lanes

AZ-1101 100 Lanes
EUR 140.4

Azura PureView 1 Kb DNA Ladder - 500 Lanes

AZ-1105 500 Lanes
EUR 277.2

Azura PureView 1 Kb DNA Ladder - 1000 Lanes

AZ-1105-2 1000 Lanes
EUR 326.4

Azura PureView 250bp DNA Ladder - 100 Lanes

AZ-1121 100 Lanes
EUR 140.4

Azura PureView 250bp DNA Ladder - 500 Lanes

AZ-1125 500 Lanes
EUR 277.2
Nicholas

About the Author

Nicholas

Leave a Reply

Your email address will not be published.