Transformation of Archaea by Direct PCR Merchandise with its Utility To Directed Evolution

Excessive-efficiency transformation of archaea by direct PCR merchandise with its utility to directed evolution of a thermostable enzyme

Hyperthermophilic archaea with distinctive biochemical and physiological traits are necessary organisms for basic analysis of life science and have nice potential for biotechnological functions. Nonetheless, low transformation effectivity of international DNA molecules impedes developments in genetic modification instruments and industrial functions.

 

  • On this research, we utilized extended overlap extension PCR (POE-PCR) to generate multimeric DNA molecules after which remodeled them into two hyperthermophilic archaea, Thermococcus kodakarensis KOD1 and Pyrococcus yayanosii A1.
  • This research was the primary instance to display the improved transformation efficiencies of POE-PCR merchandise by an element of roughly 100 for T. kodakarensis KOD1 and eight for P. yayanosii A1, respectively, relative to round shuttle plasmids.
  • Moreover, directed evolution of a modestly thermophilic enzyme, Methanothermococcus okinawensis 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), was carried out to acquire extra steady ones on account of excessive transformation effectivity of T. kodakarensis (i.e. ~3 × 104 CFU per μg DNA). T. kodakarensis harbouring essentially the most thermostable MoHMGR mutant can develop within the presence of a thermostable antibiotic simvastatin at 85°C and even greater temperatures.
  • This excessive transformation effectivity method couldn’t solely assist develop extra hyperthermophilic enzyme mutants by way of directed evolution but in addition simplify genetical modification of archaea, which could possibly be novel hosts for industrial biotechnology

Screening of business meat merchandise from grocery store chains for feline derivatives utilizing SP-PCR-RLFP and lab-on-a-chip

Willpower of feline meat in meals merchandise is a vital situation for social, well being, financial and spiritual concern. Therefore this paper documented the applying of species particular polymerase chain reaction-restriction fragment size polymorphism (SP-PCR-RFLP) assay concentrating on a short-fragments (69 bp) of mitochondrial cytochrome b (cytb) gene to display feline meat in industrial meat merchandise utilizing lab-on-a-chip.
The SP-PCR assay proved its specificity theoretically and experimentally whereas testing with totally different widespread animal, aquatic and plant species of DNA. The feline particular (69 bp, 43- and 26-bp) attribute molecular DNA sample was noticed by SP-PCR and RFLP evaluation.

For assay efficiency, it was examined in three several types of industrial dummy meat merchandise equivalent to frankfurters, nuggets and meatballs and digested with AluI-restriction enzyme. The best sensitivity of the assay utilizing lab-on-a-chip was as little as 0.1 pg or 0.01 % (w/w) in industrial dummy meat merchandise. Now we have additionally utilized this assay to display three necessary industrial meat merchandise of six totally different model from six grocery store chains positioned at three totally different states of Malaysia.

Thus whole 378 samples had been examined to validate the specificity, sensitivity, stability of the assay and utilization of it for industrial meat product screening.
Key phrases: Industrial meat merchandise; Feline meat; Halal; Lab-on-a-chip; Restriction digestion.
genpcr

genpcr

Alexa Fluor® 594 anti-mouse CD31

E16FMJ031-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-mouse CD45

E16FMJ045-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-mouse CD68

E16FMJ068-100U 100 μg
EUR 1365
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-human CD8a

E16FHJ008a-100U 100 μg
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-human CD14

E16FHJ0142-100U 100 μg
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-human CD19

E16FHJ019-100U 100 μg
EUR 1083.33
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-mouse CD11c

E16FMJ011c-100U 100 μg
EUR 758.33
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-mouse CD106

E16FMJ106-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-human CD11a

E16FHJ011a-100U 100 μg
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-human CD11b

E16FHJ011b-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Mouse Anti-Human IgA - Alexa Fluor 594

DL87387A-100ul 100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgA - Alexa Fluor 594

DL87387A-500ul 500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 594

DL87390A-100ul 100 ul
EUR 155
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 594

DL87390A-500ul 500 ul
EUR 350
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgA - Alexa Fluor 594

E38A3634 100μL
EUR 125

Mouse Anti-Human IgM - Alexa Fluor 594

E38A3638 100μL
EUR 125

Mouse Anti-Human IgM - Alexa Fluor 594

YLF0361-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 594

YLF0361-1ml 1 ml
EUR 480
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 594

YLF0361-500ul 500 ul
EUR 290
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgA - Alexa Fluor 594

YLF0564-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgA - Alexa Fluor 594

YLF0564-1ml 1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgA - Alexa Fluor 594

YLF0564-500ul 500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 594

DL87379A-100ul 100 ul
EUR 190
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 594

DL87379A-500ul 500 ul
EUR 280
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 594

E38A3625 100μL
EUR 125

Rabbit Anti-Human IgM - Alexa Fluor 594

YLF0561-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 594

YLF0561-1ml 1 ml
EUR 300
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 594

YLF0561-500ul 500 ul
EUR 210
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594

DL87358A-100ul 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594

DL87358A-500ul 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594

YLF0339-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594

YLF0339-1ml 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594

YLF0339-500ul 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 594

DL87357A-100ul 100 ul
EUR 190
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 594

DL87357A-500ul 500 ul
EUR 280
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 594

DL87389A-100ul 100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 594

DL87389A-500ul 500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Alexa Fluor® 594 anti-mouse CD206 (MMR)

E16FMJ206-100U 100 μg
EUR 1191.67
Description: Available in various conjugation types.

Goat Anti-Human IgG Fab - Alexa Fluor 594

E38A3603 100μL
EUR 125

Mouse Anti-Human IgG Fc - Alexa Fluor 594

E38A3636 100μL
EUR 125

Goat Anti-Human IgG Fab - Alexa Fluor 594

YLF0558-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 594

YLF0558-1ml 1 ml
EUR 300
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 594

YLF0558-500ul 500 ul
EUR 210
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 594

YLF0566-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 594

YLF0566-1ml 1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 594

YLF0566-500ul 500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

DL87376A-100ul 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

DL87376A-500ul 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fab - Alexa Fluor 594

DL87388A-100ul 100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fab - Alexa Fluor 594

DL87388A-500ul 500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Alexa Fluor® 594 anti-human/mouse CD44

E16FMJ044-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

E38A3622 100μL
EUR 125

Mouse Anti-Human IgG Fab - Alexa Fluor 594

E38A3635 100μL
EUR 125

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

YLF0354-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

YLF0354-1ml 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594

YLF0354-500ul 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fab - Alexa Fluor 594

YLF0565-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fab - Alexa Fluor 594

YLF0565-1ml 1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fab - Alexa Fluor 594

YLF0565-500ul 500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Alexa Fluor® 594 anti-human/mouse CD11b

E16FMJ011b-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

43R-ID022AF 500 ug
EUR 334
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

43R-ID047AF 500 ug
EUR 465
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

DL87364A-100ul 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

DL87364A-500ul 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

E38A3610 100μL
EUR 125

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0343-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0343-1ml 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0343-500ul 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Donkey anti Goat IgG (H + L) (Alexa Fluor 594)

43R-ID005AF 500 ug
EUR 333
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594)

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594

DL87393A-100ul 100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594

DL87393A-500ul 500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594

E38A3641 100μL
EUR 125

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0569-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0569-1ml 1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594

YLF0569-500ul 500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG (H&L) - Alexa Fluor 594

DL87356A-100ul 100 ul
EUR 140
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG (H&L) - Alexa Fluor 594

DL87356A-500ul 500 ul
EUR 330
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Multiplex PCR for simultaneous identification of the most typical European Opisthorchiid and Heterophyid in fish or fish merchandise

Amongst others, the households Opisthorchiidae and Heterophyidae contains a number of genera inflicting fish-borne zoonoses and distributed additionally in European Nations and which can be included within the ParaFishControl (Superior Instruments and Analysis Methods for Parasite Management in European farmed fish) H2020 EU venture.
Because of the small dimension of the metacercariae, the infective stage for human, these parasites can’t be detected visually in fish and monitoring requires professional utility of time-consuming methods. The goal of this was to develop a fast and inexpensive molecular technique primarily based on multiplex PCR for simultaneous identification of metacercariae of the most typical European Opisthorchiid and Heterophyid in fish or fish merchandise.
Key phrases: Europe; Fish merchandise; Freshwater fish; Heterophyidae; Multiplex PCR; Opisthorchiidae
TaqMan probe primarily based multiplex quantitative PCR assay for dedication of bovine, porcine and fish DNA in gelatin admixture, meals merchandise and dietary dietary supplements.
Detection of animal supplies in gelatin-based merchandise is required to deal with spiritual and cultural considerations, as a result of porcine and bovine gelatins are prohibited in Halal, Kosher and Hindus shopper goods. On this paper, multiplex quantitative polymerase chain response (qPCR) assay utilizing TaqMan probe was developed to discriminate bovine, porcine and fish gelatin species in a single assay platform.
The assay was particular to cattle, pigs and fish, having been examined in opposition to 14 non-target species. The restrict of detection, underneath gelatin admixed circumstances, was 0.005 ng/µL. Lastly, a pilot survey was undertaken testing 35 Halal branded processed meals and dietary gadgets. Out of 35 samples, solely two had been discovered to be optimistic for porcine species. The authenticity of those two qPCR merchandise was confirmed by DNA sequencing evaluation, which confirmed 99-100% similarity with Sus scrofa (Wild boar) species.

HDV Real Time RT-PCR Kit

GWB-LRB030 25 tests Ask for price

HCMV Real Time PCR Kit

GWB-LRB009 25 tests Ask for price

HCMV Real Time PCR Kit

GWB-LRB010 25 tests Ask for price

HEV IV Real Time RT-PCR Kit

GWB-LRB031 25 tests Ask for price

HEV IV Real Time RT-PCR Kit

GWB-LRB032 25 tests Ask for price

ONE STEP RT-PCR REAL TIME KIT

MBT128-10R 1 unit
EUR 37.4
Description: ONE STEP RT-PCR REAL TIME KIT

ONE STEP RT-PCR REAL TIME KIT

MBT128-25R 1 unit
EUR 89.72
Description: ONE STEP RT-PCR REAL TIME KIT

ONE STEP RT-PCR REAL TIME KIT

MBT128-2X50R 1 unit
EUR 314.51
Description: ONE STEP RT-PCR REAL TIME KIT

ONE STEP RT-PCR REAL TIME KIT

MBT128-50R 1 unit
EUR 165.56
Description: ONE STEP RT-PCR REAL TIME KIT

HCV Genetype Real Time RT-PCR Kit

GWB-LRB027 25 tests Ask for price

HCV Genetype Real Time RT-PCR Kit

GWB-LRB028 25 tests Ask for price

RV Real Time RT-PCR Detection kit

TRI-L02M1 32T
EUR 705.6

RV Real Time RT-PCR Detection kit

TRI-L02S1 16T
EUR 378

Measles Virus Real Time RT-PCR Kit

GWB-LRB043 25 tests Ask for price

Measles Virus Real Time RT-PCR Kit

GWB-LRB044 25 tests Ask for price

Rubella Virus Real Time RT-PCR Kit

GWB-LRB045 25 tests Ask for price

Rubella Virus Real Time RT-PCR Kit

GWB-LRB046 25 tests Ask for price

FCV Real Time RT-PCR Detection kit

TRI-L14M1 32T
EUR 705.6

FCV Real Time RT-PCR Detection kit

TRI-L14S1 16T
EUR 378

FIV Real Time RT-PCR Detection kit

TRI-L22M1 32T
EUR 705.6

FIV Real Time RT-PCR Detection kit

TRI-L22S1 16T
EUR 378

HLA B27 Real-TM Real Time PCR kit

R116-50FRT 48
EUR 627.84

FIPV Real Time RT-PCR Detection kit

TRI-L03M1 32T
EUR 453.6

FIPV Real Time RT-PCR Detection kit

TRI-L03S1 16T
EUR 252

CPIV Real Time RT-PCR Detection kit

TRI-L07M1 32T
EUR 453.6

CPIV Real Time RT-PCR Detection kit

TRI-L07S1 16T
EUR 252

CSFV Real Time RT-PCR Detection kit

TRI-L28M1 48T
EUR 1008

CSFV Real Time RT-PCR Detection kit

TRI-L28S1 24T
EUR 529.2

PEDV Real Time RT-PCR Detection kit

TRI-L34M1 48T
EUR 1008

PEDV Real Time RT-PCR Detection kit

TRI-L34S1 24T
EUR 529.2

CP&MP Real Time PCR Kit

GWB-LRB005 25 tests Ask for price

CP&MP Real Time PCR Kit

GWB-LRB006 25 tests Ask for price

MRSA Quant Real-TM Real Time PCR kit

B78-100FRT 100
EUR 784.8

Chikungunya Virus Real Time RT-PCR Kit

GWB-LRB001 25 tests Ask for price

Chikungunya Virus Real Time RT-PCR Kit

GWB-LRB002 25 tests Ask for price

Yersinia pestis Real-TM Real Time PCR kit

B79-50FRT 50
EUR 627.84

Monkeypox Virus Real Time PCR Kit

PDPS-AR064 1 unit Ask for price
Description: Creative Biogene Monkeypox Virus Real Time PCR Kit is used for the detection of monkeypox Virus in serum or lesion exudate samples by using real time PCR systems. Monkeypox virus (MPV) is a double-stranded DNA, zoonotic virus and a species of the genus Orthopoxvirus in the family Poxviridae. It is one of the human orthopoxviruses that includes variola (VARV), cowpox (CPX), and vaccinia (VACV) viruses. The kit contains a specific ready-to-use system for the detection of the monkeypox Virus. Fluorescence is emitted and measured by the real time systems' optical unit during the PCR.

VetAlert Johnes Real Time PCR Kit

TC-9828-100 100 rxns
EUR 460

Monkeypox Virus Real Time PCR Kit

ZD-0076-01 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit

ZD-0076-02 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit

YJC70115NW-25T 25 tests/kit Ask for price
Description: The Bioperfectus Monkeypox Virus Real Time PCR Kit is an in vitro diagnostic test, based on real-time PCR technology, for the detection of DNA from the Monkeypox virus. Specimens can be obtained from human serum, lesion exudate samples and scab. BSL-2 facilities with standard BSL-2 work practices may be used for the test of t he Monkeypox virus.

Human Lung Cancer PCR Primer Library

HLUCPL-I 1 set
EUR 657.6

Human Schwann Cell PCR Primer Library

HSCH-I 1 set
EUR 657.6

HBV Quantitative Real Time PCR Kit

GWB-LRB013 25 tests Ask for price

HBV Quantitative Real Time PCR Kit

GWB-LRB014 25 tests Ask for price

Staphilococcus aureus Real-TM Real Time PCR kit

B1772-96FRT 96
EUR 562.44

Toxoplasma Gondii Real Time PCR Kit

GWB-LRB049 25 tests Ask for price

Toxoplasma Gondii Real Time PCR Kit

GWB-LRB050 25 tests Ask for price

HEV I & III Real Time RT-PCR Kit

GWB-LRB033 25 tests Ask for price

HEV I & III Real Time RT-PCR Kit

GWB-LRB034 25 tests Ask for price

CHV Real Time PCR Detection kit

TRI-L09M1 32T
EUR 453.6

CHV Real Time PCR Detection kit

TRI-L09S1 16T
EUR 252

PCVⅡ Real Time PCR Detection kit

TRI-L29M1 48T
EUR 705.6

PCVⅡ Real Time PCR Detection kit

TRI-L29S1 24T
EUR 378

HEV General Type Real Time RT-PCR Kit

GWB-LRB037 25 tests Ask for price

HEV General Type Real Time RT-PCR Kit

GWB-LRB038 25 tests Ask for price

BioRT Real Time RT-PCR Kit(SYBR Green)

TRI-B33M1 100T
EUR 1058.4

BioRT Real Time RT-PCR Kit(SYBR Green)

TRI-B33S1 50T
EUR 554.4

ASFV Real Time PCR Detection kit

TRI-L04M1C 48T
EUR 554.4

ASFV Real Time PCR Detection kit

TRI-L04S1C 24T
EUR 302.4

Babesia Real Time PCR Detection kit

TRI-L20M1 32T
EUR 453.6

Babesia Real Time PCR Detection kit

TRI-L20S1 16T
EUR 252

Giardia Real Time PCR Detection kit

TRI-L26M1 32T
EUR 453.6

Giardia Real Time PCR Detection kit

TRI-L26S1 16T
EUR 252

Oncogenetics BRCA Panel Real-TM Real Time PCR kit

R-27-P-48FRT 48
EUR 1907.5

Brucella Real Time PCR Detection kit

TRI-L24M1 32T
EUR 453.6

Brucella Real Time PCR Detection kit

TRI-L24S1 16T
EUR 252

Chlamydia Real Time PCR Detection kit

TRI-L15M1 32T
EUR 453.6

Chlamydia Real Time PCR Detection kit

TRI-L15S1 16T
EUR 252

Leptospira Real Time PCR Detection kit

TRI-L13M1 32T
EUR 453.6

Leptospira Real Time PCR Detection kit

TRI-L13S1 16T
EUR 252

Mycoplasma Real Time PCR Detection kit

TRI-L16M1 32T
EUR 453.6

Mycoplasma Real Time PCR Detection kit

TRI-L16S1 16T
EUR 252

Ehrilichia Real Time PCR Detection kit

TRI-L25M1 32T
EUR 453.6

Ehrilichia Real Time PCR Detection kit

TRI-L25S1 16T
EUR 252

PRRSV Universal Real Time RT-PCR Detection kit

TRI-L33M1 48T
EUR 1008

PRRSV Universal Real Time RT-PCR Detection kit

TRI-L33S1 24T
EUR 529.2

Edwardsiella Real Time PCR Detection kit

TRI-N14M1 32T
EUR 491.4

Edwardsiella Real Time PCR Detection kit

TRI-N14S1 16T
EUR 289.8

HCV/HBV/HIV1/HIV2 Real-TM Real Time PCR kit

V62-100FRT 100
EUR 1560.88

Chlamydia Pheumoniae (CP) Real Time PCR Kit

GWB-LRB003 25 tests Ask for price

Chlamydia Pheumoniae (CP) Real Time PCR Kit

GWB-LRB004 25 tests Ask for price

PRV (gE) Real Time PCR Detection kit

TRI-L30M1 48T
EUR 705.6

PRV (gE) Real Time PCR Detection kit

TRI-L30S1 24T
EUR 378

COMT Val158Met SNP-Screen Real-TM Real Time PCR kit

T01352-50-T 60
EUR 538.46

FHV-1 Real Time PCR Detection kit

TRI-L01M1 32T
EUR 453.6

FHV-1 Real Time PCR Detection kit

TRI-L01S1 16T
EUR 252

BP/MP/CP Multiplex Real Time PCR Kit

GWB-LRB007 25 tests Ask for price

BP/MP/CP Multiplex Real Time PCR Kit

GWB-LRB008 25 tests Ask for price

Fcov and Ccov Real Time RT-PCR Detection kit

TRI-L11M1 32T
EUR 705.6

Fcov and Ccov Real Time RT-PCR Detection kit

TRI-L11S1 16T
EUR 378
Nicholas

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