Excessive-efficiency transformation of archaea by direct PCR merchandise with its utility to directed evolution of a thermostable enzyme
Hyperthermophilic archaea with distinctive biochemical and physiological traits are necessary organisms for basic analysis of life science and have nice potential for biotechnological functions. Nonetheless, low transformation effectivity of international DNA molecules impedes developments in genetic modification instruments and industrial functions.
- On this research, we utilized extended overlap extension PCR (POE-PCR) to generate multimeric DNA molecules after which remodeled them into two hyperthermophilic archaea, Thermococcus kodakarensis KOD1 and Pyrococcus yayanosii A1.
- This research was the primary instance to display the improved transformation efficiencies of POE-PCR merchandise by an element of roughly 100 for T. kodakarensis KOD1 and eight for P. yayanosii A1, respectively, relative to round shuttle plasmids.
- Moreover, directed evolution of a modestly thermophilic enzyme, Methanothermococcus okinawensis 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), was carried out to acquire extra steady ones on account of excessive transformation effectivity of T. kodakarensis (i.e. ~3 × 104 CFU per μg DNA). T. kodakarensis harbouring essentially the most thermostable MoHMGR mutant can develop within the presence of a thermostable antibiotic simvastatin at 85°C and even greater temperatures.
- This excessive transformation effectivity method couldn’t solely assist develop extra hyperthermophilic enzyme mutants by way of directed evolution but in addition simplify genetical modification of archaea, which could possibly be novel hosts for industrial biotechnology
Screening of business meat merchandise from grocery store chains for feline derivatives utilizing SP-PCR-RLFP and lab-on-a-chip
Willpower of feline meat in meals merchandise is a vital situation for social, well being, financial and spiritual concern. Therefore this paper documented the applying of species particular polymerase chain reaction-restriction fragment size polymorphism (SP-PCR-RFLP) assay concentrating on a short-fragments (69 bp) of mitochondrial cytochrome b (cytb) gene to display feline meat in industrial meat merchandise utilizing lab-on-a-chip.
The SP-PCR assay proved its specificity theoretically and experimentally whereas testing with totally different widespread animal, aquatic and plant species of DNA. The feline particular (69 bp, 43- and 26-bp) attribute molecular
DNA sample was noticed by SP-PCR and RFLP evaluation.
For assay efficiency, it was examined in three several types of industrial dummy meat merchandise equivalent to frankfurters, nuggets and meatballs and digested with AluI-restriction enzyme. The best sensitivity of the assay utilizing lab-on-a-chip was as little as 0.1 pg or 0.01 % (w/w) in industrial dummy meat merchandise. Now we have additionally utilized this assay to display three necessary industrial meat merchandise of six totally different model from six grocery store chains positioned at three totally different states of Malaysia.
Thus whole 378 samples had been examined to validate the specificity, sensitivity, stability of the assay and utilization of it for industrial meat product screening.
Key phrases:Â Industrial meat merchandise; Feline meat; Halal; Lab-on-a-chip; Restriction digestion.

genpcr
XFD594 Phalloidin *XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
23158 |
AAT Bioquest |
300 Tests |
EUR 334 |
Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated |
Ab8011-001 |
GenDepot |
1mg |
EUR 400.8 |
SAM FCM (Alexa Fluor 647) |
abx098902-100tests |
Abbexa |
100 tests |
EUR 1479.6 |
|
SAM FCM (Alexa Fluor 488) |
abx098904-60tests |
Abbexa |
60 tests |
EUR 1629.6 |
|
Endoglin/CD105 Alexa Fluor |
FC15024 |
Neuromics |
100 Tests |
EUR 537.6 |
XFD594 tyramide reagent *Same Structure to Alexa Fluorâ„¢ 594 tyramide* |
11082 |
AAT Bioquest |
200 slides |
EUR 222 |
EGFR antibody (Alexa Fluor 488) |
61R-E109BAF |
Fitzgerald |
125 ug |
EUR 847.2 |
Description: Mouse monoclonal EGFR antibody (Alexa Fluor 488) |
XFD594 NHS Ester *Same Structure to Alexa Fluorâ„¢ 594 NHS Ester* |
1830 |
AAT Bioquest |
1 mg |
EUR 222 |
XFD594 NHS Ester *Same Structure to Alexa Fluorâ„¢ 594 NHS Ester* |
71908 |
AAT Bioquest |
5 mg |
EUR 781 |
XFD594-streptavidin conjugate *XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
16892 |
AAT Bioquest |
1 mg |
EUR 166 |
XFD594 C5 Maleimide *Same Structure to Alexa Fluorâ„¢ 594 C5 Maleimide* |
1891 |
AAT Bioquest |
1 mg |
EUR 222 |
Annexin V, XFD594 conjugate *XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
20096 |
AAT Bioquest |
100 tests |
EUR 222 |
Anti-RPSA Alexa Fluor® 488 |
A4-829-C100 |
ExBio |
0.1 mg |
EUR 372 |
Concanavalin A, XFD594 Labeled *XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
25575 |
AAT Bioquest |
1 mg |
EUR 102 |
Wheat Germ Agglutinin, XFD594 Labeled *XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
25509 |
AAT Bioquest |
1 mg |
EUR 102 |
Goat anti Mouse IgG1 (Alexa Fluor 488) |
43R-1649 |
Fitzgerald |
500 ug |
EUR 684 |
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488) |
XFD594 Anti-human CD3 Antibody *SK7, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10033170 |
AAT Bioquest |
100 tests |
EUR 245 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD3 Antibody *SK7, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10033171 |
AAT Bioquest |
500 tests |
EUR 918 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD4 Antibody *SK3, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10042170 |
AAT Bioquest |
100 tests |
EUR 245 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD4 Antibody *SK3, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10042171 |
AAT Bioquest |
500 tests |
EUR 918 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD8 Antibody *SK1, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10081170 |
AAT Bioquest |
100 tests |
EUR 245 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD8 Antibody *SK1, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10081171 |
AAT Bioquest |
500 tests |
EUR 918 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD28 Antibody *9.3, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10281170 |
AAT Bioquest |
100 tests |
EUR 245 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD28 Antibody *9.3, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10281171 |
AAT Bioquest |
500 tests |
EUR 918 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD64 Antibody *10.1, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10640170 |
AAT Bioquest |
100 tests |
EUR 245 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD64 Antibody *10.1, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10640171 |
AAT Bioquest |
500 tests |
EUR 918 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD4 Antibody *SK3, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10042170-100tests |
AAT Bioquest |
100 tests |
EUR 245 |
Description: SK3 is an anti-human monoclonal antibody that recognizes the CD4 antigen. |
XFD594 Anti-human CD4 Antibody *SK3, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10042171-500tests |
AAT Bioquest |
500 tests |
EUR 918 |
Description: SK3 is an anti-human monoclonal antibody that recognizes the CD4 antigen. |
XFD594 Anti-human CD8 Antibody *SK1, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10081170-100tests |
AAT Bioquest |
100 tests |
EUR 245 |
Description: SK1 is an anti-human monoclonal antibody that is specific for the CD8a antigen. |
XFD594 Anti-human CD8 Antibody *SK1, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10081171-500tests |
AAT Bioquest |
500 tests |
EUR 918 |
Description: SK1 is an anti-human monoclonal antibody that is specific for the CD8a antigen. |
XFD594 Anti-human CD3 Antibody *SK7, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10033170-100tests |
AAT Bioquest |
100 tests |
EUR 245 |
Description: The SK7 monoclonal antibody reacts with human CD3e, a 20 kD member of the Ig superfamily often found on the surface of t cells, tregs and nkt cells. |
XFD594 Anti-human CD3 Antibody *SK7, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10033171-500tests |
AAT Bioquest |
500 tests |
EUR 918 |
Description: The SK7 monoclonal antibody reacts with human CD3e, a 20 kD member of the Ig superfamily often found on the surface of t cells, tregs and nkt cells. |
XFD594 Anti-human CD64 Antibody *10.1, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10640170-100tests |
AAT Bioquest |
100 tests |
EUR 245 |
Description: The 10.1 monoclonal antibody binds with human CD64, a 72 kD single-pass type i membrane protein typically expressed on the surface of granulocytes, monocytes and dendritic cells. |
XFD594 Anti-human CD64 Antibody *10.1, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10640171-500tests |
AAT Bioquest |
500 tests |
EUR 918 |
Description: The 10.1 monoclonal antibody binds with human CD64, a 72 kD single-pass type i membrane protein typically expressed on the surface of granulocytes, monocytes and dendritic cells. |
XFD594 Anti-human CD28 Antibody *9.3, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10281170-100tests |
AAT Bioquest |
100 tests |
EUR 245 |
Description: The 9.3 monoclonal antibody binds with human CD28, a 44 kD transmembrane glycoprotein typically located on the surface of natural killer cells and plasma cells. |
XFD594 Anti-human CD28 Antibody *9.3, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10281171-500tests |
AAT Bioquest |
500 tests |
EUR 918 |
Description: The 9.3 monoclonal antibody binds with human CD28, a 44 kD transmembrane glycoprotein typically located on the surface of natural killer cells and plasma cells. |
XFD594 Anti-human CD11c Antibody *3.9, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10113170 |
AAT Bioquest |
100 tests |
EUR 245 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD11c Antibody *3.9, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10113171 |
AAT Bioquest |
500 tests |
EUR 918 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD1 Antibody *SN13, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10012160 |
AAT Bioquest |
100 tests |
EUR 547 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD1 Antibody *SN13, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10012161 |
AAT Bioquest |
500 tests |
EUR 2051 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD1 Antibody *L161, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10013160 |
AAT Bioquest |
100 tests |
EUR 547 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD1 Antibody *L161, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10013161 |
AAT Bioquest |
500 tests |
EUR 2051 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD9 Antibody *HI9a, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10090170 |
AAT Bioquest |
100 tests |
EUR 245 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD9 Antibody *HI9a, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10090171 |
AAT Bioquest |
500 tests |
EUR 918 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD7 Antibody *HIT7, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10070170 |
AAT Bioquest |
100 tests |
EUR 245 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD7 Antibody *HIT7, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10070171 |
AAT Bioquest |
500 tests |
EUR 918 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD16 Antibody *3G8, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10161170 |
AAT Bioquest |
100 tests |
EUR 245 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD16 Antibody *3G8, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10161171 |
AAT Bioquest |
500 tests |
EUR 918 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD19 Antibody *4G7, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10193170 |
AAT Bioquest |
100 tests |
EUR 245 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD19 Antibody *4G7, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10193171 |
AAT Bioquest |
500 tests |
EUR 918 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-mouse CD19 Antibody *1D3, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10194160 |
AAT Bioquest |
100 tests |
EUR 245 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-mouse CD19 Antibody *1D3, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10194161 |
AAT Bioquest |
500 tests |
EUR 918 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD38 Antibody *HB7, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10382160 |
AAT Bioquest |
100 tests |
EUR 245 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD38 Antibody *HB7, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10382161 |
AAT Bioquest |
500 tests |
EUR 918 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD40 Antibody *G28.5, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10401171 |
AAT Bioquest |
500 tests |
EUR 918 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD32 Antibody *3D3, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10321170 |
AAT Bioquest |
100 tests |
EUR 547 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD32 Antibody *3D3, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10321171 |
AAT Bioquest |
500 tests |
EUR 2051 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD45 Antibody *2D1, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10454170 |
AAT Bioquest |
100 tests |
EUR 245 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD45 Antibody *2D1, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10454171 |
AAT Bioquest |
500 tests |
EUR 918 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD72 Antibody *3F3, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10720170 |
AAT Bioquest |
100 tests |
EUR 547 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD72 Antibody *3F3, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10720171 |
AAT Bioquest |
500 tests |
EUR 2051 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD73 Antibody *AD2, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10730170 |
AAT Bioquest |
100 tests |
EUR 547 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD73 Antibody *AD2, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10730171 |
AAT Bioquest |
500 tests |
EUR 2051 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD82 Antibody *C33, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10820170 |
AAT Bioquest |
100 tests |
EUR 547 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD82 Antibody *C33, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10820171 |
AAT Bioquest |
500 tests |
EUR 2051 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD111 Antibody *R1.302, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
11110160 |
AAT Bioquest |
100 tests |
EUR 547 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD111 Antibody *R1.302, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
11110161 |
AAT Bioquest |
500 tests |
EUR 2051 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD112 Antibody *R2.525, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
11120160 |
AAT Bioquest |
100 tests |
EUR 547 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD112 Antibody *R2.525, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
11120161 |
AAT Bioquest |
500 tests |
EUR 2051 |
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn |
XFD594 Anti-human CD7 Antibody *HIT7, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10070170-100tests |
AAT Bioquest |
100 tests |
EUR 245 |
Description: HIT7 is an anti-human monoclonal antibody that recognizes the CD7 antigen. |
XFD594 Anti-human CD7 Antibody *HIT7, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10070171-500tests |
AAT Bioquest |
500 tests |
EUR 918 |
Description: HIT7 is an anti-human monoclonal antibody that recognizes the CD7 antigen. |
XFD594 Anti-human CD9 Antibody *HI9a, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10090170-100tests |
AAT Bioquest |
100 tests |
EUR 245 |
Description: HI9a is an anti-human monoclonal antibody that recognizes the CD9 antigen. |
XFD594 Anti-human CD9 Antibody *HI9a, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10090171-500tests |
AAT Bioquest |
500 tests |
EUR 918 |
Description: HI9a is an anti-human monoclonal antibody that recognizes the CD9 antigen. |
XFD594 Anti-human CD11c Antibody *3.9, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10113170-100tests |
AAT Bioquest |
100 tests |
EUR 245 |
Description: 3.9 is an anti-human monoclonal antibody that forms an immune complex with the CD11c antigen. |
XFD594 Anti-human CD11c Antibody *3.9, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10113171-500tests |
AAT Bioquest |
500 tests |
EUR 918 |
Description: 3.9 is an anti-human monoclonal antibody that forms an immune complex with the CD11c antigen. |
XFD594 Anti-human CD16 Antibody *3G8, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10161170-100tests |
AAT Bioquest |
100 tests |
EUR 245 |
Description: The 3G8 monoclonal antibody recognizes human CD16a, a 50 - 65 kD transmembrane protein typically found on the surface of macrophages, natural killer cells, dendritic cells, monocytes and neutrophils. |
XFD594 Anti-human CD16 Antibody *3G8, XFD594 Same Structure to Alexa Fluorâ„¢ 594* |
10161171-500tests |
AAT Bioquest |
500 tests |
EUR 918 |
Description: The 3G8 monoclonal antibody recognizes human CD16a, a 50 - 65 kD transmembrane protein typically found on the surface of macrophages, natural killer cells, dendritic cells, monocytes and neutrophils. |
Multiplex PCR for simultaneous identification of the most typical European Opisthorchiid and Heterophyid in fish or fish merchandise
Amongst others, the households Opisthorchiidae and Heterophyidae contains a number of genera inflicting fish-borne zoonoses and distributed additionally in European Nations and which can be included within the ParaFishControl (Superior Instruments and Analysis Methods for Parasite Management in European farmed fish) H2020 EU venture.
Because of the small dimension of the metacercariae, the infective stage for human, these parasites can’t be detected visually in fish and monitoring requires professional utility of time-consuming methods. The goal of this was to develop a fast and inexpensive molecular technique primarily based on multiplex PCR for simultaneous identification of metacercariae of the most typical European Opisthorchiid and Heterophyid in fish or fish merchandise.
Key phrases:Â Europe; Fish merchandise; Freshwater fish; Heterophyidae; Multiplex PCR; Opisthorchiidae
TaqMan probe primarily based multiplex quantitative PCR assay for dedication of bovine, porcine and fish DNA in gelatin admixture, meals merchandise and dietary dietary supplements.
Detection of animal supplies in gelatin-based merchandise is required to deal with spiritual and cultural considerations, as a result of porcine and bovine gelatins are prohibited in Halal, Kosher and Hindus shopper goods. On this paper, multiplex quantitative polymerase chain response (qPCR) assay utilizing TaqMan probe was developed to discriminate bovine, porcine and fish gelatin species in a single assay platform.
The assay was particular to cattle, pigs and fish, having been examined in opposition to 14 non-target species. The restrict of detection, underneath gelatin admixed circumstances, was 0.005 ng/µL. Lastly, a pilot survey was undertaken testing 35 Halal branded processed meals and dietary gadgets. Out of 35 samples, solely two had been discovered to be optimistic for porcine species. The authenticity of those two qPCR merchandise was confirmed by DNA sequencing evaluation, which confirmed 99-100% similarity with Sus scrofa (Wild boar) species.
Eztime Real - time PCRPremix 2x,Taqman |
FYT105-100P |
Yeastern Biotech |
100 Preps 1.25 ml |
Ask for price |
AzuraQuant cDNA Synthesis Kit - 25 Reactions |
AZ-1995 |
Real Time Primers |
25 Reactions |
EUR 183.6 |
Eztime Real - time PCRPremix 2x,SYBR Green |
FYT103-100P |
Yeastern Biotech |
100 Preps 1.25 ml |
Ask for price |
Eztime Real - time PCRPremix 2x,SYBR Green |
FYT103-400P |
Yeastern Biotech |
400 Preps 1.25 ml x 4 |
Ask for price |
Eztime Real - time PCRPremix 2x,Taqman, ROX |
FYT105-400P |
Yeastern Biotech |
400 Preps 1.25 ml x 4 |
Ask for price |
Eztime Real - time PCRPremix 2x,Taqman, ROX |
FYT106-100P |
Yeastern Biotech |
100 Preps 1.25 ml |
Ask for price |
Eztime Real - time PCRPremix 2x,SYBRGreen , ROX |
FYT104-100P |
Yeastern Biotech |
100 Preps 1.25 ml |
Ask for price |
Eztime Real - time PCRPremix 2x,SYBRGreen , ROX |
FYT104-400P |
Yeastern Biotech |
400 Preps 1.25 ml x 4 |
Ask for price |
hsa-mir-521 Real-time RT-PCR Detection Kit |
20-abx097600 |
Abbexa |
-
EUR 661.20
-
EUR 944.40
-
EUR 477.60
|
- 100 rxns
- 200 rxns
- 50 rxns
|
|
Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye |
MB1004-100Reactions |
BosterBio |
100 Reactions |
EUR 392.4 |
Novel Coronavirus COVID-19 (2019-nCoV) Real Time Multiplex RT-PCR Kit (Detection for 3 Genes ) |
RR-0479-02 |
Liferiver |
25 tests/kit |
EUR 469.56 |
|
Description: Novel Coronavirus (2019-nCoV) Real Time Multiplex RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems. It detects N gene, E gene and RdRp gene of 2019-nCoV. RR-0479-02 has been also approverd by CFDA for emergency use and is WHO standard. |
dsGreen for Real-Time PCR, 100×, 1 mL |
41010 |
Lumiprobe |
1 mL |
EUR 298.8 |
dsGreen for Real-Time PCR, 100×, 10 mL |
81010 |
Lumiprobe |
10 mL |
EUR 1042.8 |
dsGreen for Real-Time PCR, 100×, 5 mL |
71010 |
Lumiprobe |
5 mL |
EUR 750 |
dsGreen for Real-Time PCR, 100×, 2 mL |
61010 |
Lumiprobe |
2 mL |
EUR 435.6 |
Azura PureView 1 Kb DNA Ladder - 100 Lanes |
AZ-1101 |
Real Time Primers |
100 Lanes |
EUR 140.4 |
Azura PureView 1 Kb DNA Ladder - 500 Lanes |
AZ-1105 |
Real Time Primers |
500 Lanes |
EUR 277.2 |
Azura PureView 1 Kb DNA Ladder - 1000 Lanes |
AZ-1105-2 |
Real Time Primers |
1000 Lanes |
EUR 326.4 |
Azura PureView 250bp DNA Ladder - 100 Lanes |
AZ-1121 |
Real Time Primers |
100 Lanes |
EUR 140.4 |
Azura PureView 250bp DNA Ladder - 500 Lanes |
AZ-1125 |
Real Time Primers |
500 Lanes |
EUR 277.2 |
Azura PureView 250bp DNA Ladder - 1000 Lanes |
AZ-1125-2 |
Real Time Primers |
1000 Lanes |
EUR 326.4 |
Azura PureView 100bp DNA Ladder - 100 Lanes |
AZ-1131 |
Real Time Primers |
100 Lanes |
EUR 140.4 |
Azura PureView 100bp DNA Ladder - 500 Lanes |
AZ-1135 |
Real Time Primers |
500 Lanes |
EUR 277.2 |
Azura PureView 100bp DNA Ladder - 1000 Lanes |
AZ-1135-2 |
Real Time Primers |
1000 Lanes |
EUR 326.4 |
Azura PureView 50bp DNA Ladder - 100 Lanes |
AZ-1151 |
Real Time Primers |
100 Lanes |
EUR 140.4 |
Azura PureView 50bp DNA Ladder - 500 Lanes |
AZ-1155 |
Real Time Primers |
500 Lanes |
EUR 277.2 |
Azura PureView 50bp DNA Ladder - 1000 Lanes |
AZ-1155-2 |
Real Time Primers |
1000 Lanes |
EUR 326.4 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 200 Rxn |
AZ-2301 |
Real Time Primers |
200 Rxn |
EUR 214.8 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 500 Rxn |
AZ-2305 |
Real Time Primers |
500 Rxn |
EUR 384 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 2000 Rxn |
AZ-2320 |
Real Time Primers |
2000 Rxn |
EUR 1195.2 |
AzuraView GreenFast qPCR Blue Mix Lo Rox - 5000 Rxn |
AZ-2325 |
Real Time Primers |
5000 Rxn |
EUR 2817.6 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 200 Rxn |
AZ-2401 |
Real Time Primers |
200 Rxn |
EUR 214.8 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 500 Rxn |
AZ-2405 |
Real Time Primers |
500 Rxn |
EUR 384 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 2000 Rxn |
AZ-2420 |
Real Time Primers |
2000 Rxn |
EUR 1195.2 |
AzuraView GreenFast qPCR Blue Mix Hi Rox - 5000 Rxn |
AZ-2421 |
Real Time Primers |
5000 Rxn |
EUR 2817.6 |
Mouse Neurotransmitter Signaling Primer Library |
MNTS-I |
Real Time Primers |
1 set |
EUR 657.6 |
Mouse T-Cell Receptor Signaling Primer Library |
MTCR-I |
Real Time Primers |
1 set |
EUR 657.6 |
Mouse Tumor Invasion/Metastasis Primer Library |
MTIM-I |
Real Time Primers |
1 set |
EUR 540 |
Mouse Toll-like Receptor Signaling Primer Library |
newitem |
Real Time Primers |
1 set |
EUR 657.6 |
Mouse Adherin - Tight Junction Primer Library |
MATJ-I |
Real Time Primers |
1 set |
EUR 657.6 |
Mouse Cytokine and Chemokine Receptor Primer Library |
MCCR-I |
Real Time Primers |
1 set |
EUR 774 |
Mouse Epithelial-Mesenchymal Transition Primer Library |
MEMT-I |
Real Time Primers |
1 set |
EUR 657.6 |
Mouse Myeloid Derived Suppressor Cell Primer Library |
MMDSC-I |
Real Time Primers |
1 set |
EUR 657.6 |
Human Epithelial Mesenchymal Transition Primer Library |
HEMT-I |
Real Time Primers |
1 set |
EUR 657.6 |
Human Focal Adhesion Signaling Primer Library |
HFCA-I |
Real Time Primers |
1 set |
EUR 657.6 |
Human Fatty Acid and Lipid Metabolism Primer Library |
HFLM-I |
Real Time Primers |
1 set |
EUR 657.6 |
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