Transformation of Archaea by Direct PCR Merchandise with its Utility To Directed Evolution

Excessive-efficiency transformation of archaea by direct PCR merchandise with its utility to directed evolution of a thermostable enzyme

Hyperthermophilic archaea with distinctive biochemical and physiological traits are necessary organisms for basic analysis of life science and have nice potential for biotechnological functions. Nonetheless, low transformation effectivity of international DNA molecules impedes developments in genetic modification instruments and industrial functions.

On this research, we utilized extended overlap extension PCR (POE-PCR) to generate multimeric DNA molecules after which remodeled them into two hyperthermophilic archaea, Thermococcus kodakarensis KOD1 and Pyrococcus yayanosii A1.
This research was the primary instance to display the improved transformation efficiencies of POE-PCR merchandise by an element of roughly 100 for T. kodakarensis KOD1 and eight for P. yayanosii A1, respectively, relative to round shuttle plasmids.
Moreover, directed evolution of a modestly thermophilic enzyme, Methanothermococcus okinawensis 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), was carried out to acquire extra steady ones on account of excessive transformation effectivity of T. kodakarensis (i.e. ~3 × 10⁴ CFU per μg DNA). T. kodakarensis harbouring essentially the most thermostable MoHMGR mutant can develop within the presence of a thermostable antibiotic simvastatin at 85°C and even greater temperatures.
This excessive transformation effectivity method couldn’t solely assist develop extra hyperthermophilic enzyme mutants by way of directed evolution but in addition simplify genetical modification of archaea, which could possibly be novel hosts for industrial biotechnology

Screening of business meat merchandise from grocery store chains for feline derivatives utilizing SP-PCR-RLFP and lab-on-a-chip

Willpower of feline meat in meals merchandise is a vital situation for social, well being, financial and spiritual concern. Therefore this paper documented the applying of species particular polymerase chain reaction-restriction fragment size polymorphism (SP-PCR-RFLP) assay concentrating on a short-fragments (69 bp) of mitochondrial cytochrome b (cytb) gene to display feline meat in industrial meat merchandise utilizing lab-on-a-chip.

The SP-PCR assay proved its specificity theoretically and experimentally whereas testing with totally different widespread animal, aquatic and plant species of DNA. The feline particular (69 bp, 43- and 26-bp) attribute molecular DNA sample was noticed by SP-PCR and RFLP evaluation.

For assay efficiency, it was examined in three several types of industrial dummy meat merchandise equivalent to frankfurters, nuggets and meatballs and digested with AluI-restriction enzyme. The best sensitivity of the assay utilizing lab-on-a-chip was as little as 0.1 pg or 0.01 % (w/w) in industrial dummy meat merchandise. Now we have additionally utilized this assay to display three necessary industrial meat merchandise of six totally different model from six grocery store chains positioned at three totally different states of Malaysia.

Thus whole 378 samples had been examined to validate the specificity, sensitivity, stability of the assay and utilization of it for industrial meat product screening.

Key phrases: Industrial meat merchandise; Feline meat; Halal; Lab-on-a-chip; Restriction digestion.

genpcr

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)
43R-ID047AF	Fitzgerald	500 ug	EUR 462
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 594)
43R-ID056AF	Fitzgerald	500 ug	EUR 343
Description: Donkey anti Chicken IgY secondary antibody (H + L) (Alexa Fluor 594)

Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594)
43R-IR016AF	Fitzgerald	1 mg	EUR 281
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594)

Endoglin/CD105 Alexa Fluor
FC15024	Neuromics	100 Tests	EUR 448

Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated
S0005	Affbiotech	200ul	EUR 376

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 594–conjugated
S0006	Affbiotech	200ul	EUR 376

Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594)
43R-ID012AF	Fitzgerald	300 ug	EUR 410
Description: Donkey anti Goat IgG (H + L) secondary antibody (Fab'2) (Alexa Fluor 594)

Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated
Ab8011-001	GenDepot	1mg	EUR 334

EGFR antibody (Alexa Fluor 488)
61R-E109BAF	Fitzgerald	125 ug	EUR 706
Description: Mouse monoclonal EGFR antibody (Alexa Fluor 488)

SAM FCM (Alexa Fluor 647)
abx098902-100tests	Abbexa	100 tests	EUR 1233
Shipped within 5-10 working days.

SAM FCM (Alexa Fluor 488)
abx098904-60tests	Abbexa	60 tests	EUR 1358
Shipped within 5-10 working days.

Anti-RPSA Alexa Fluor® 488
A4-829-C100	ExBio	0.1 mg	EUR 310

Anti-CD40 antibody (Alexa-fluor 488)
STJ170000	St John's Laboratory	100 µg	EUR 393
Description: CD40 (48 to 50 kDa) is a transmembrane glycoprotein mainly expressed on the surface of B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 is the receptor for CD40 ligand. CD40L (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40, predominantly on activated CD4+ T cells. Following interaction with CD40 ligand, CD40 mediates a number of major immunoregulatory functions, central to the control of thymus dependent humoral immunity and may be critical in the development of cell mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin

Anti-LAMP3 antibody (Alexa-fluor 488)
STJ170004	St John's Laboratory	100 µg	EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-LAMP3 antibody (Alexa-fluor 546)
STJ170005	St John's Laboratory	100 µg	EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-LAMP3 antibody (Alexa-fluor 647)
STJ170006	St John's Laboratory	100 µg	EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-IL3RA antibody (Alexa-fluor 488)
STJ170009	St John's Laboratory	100 µg	EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ‚Äö√†√∂‚àö√¢‚Äö√¢√†‚Äö√†√® subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-IL3RA antibody (Alexa-fluor 546)
STJ170010	St John's Laboratory	100 µg	EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ‚Äö√†√∂‚àö√¢‚Äö√¢√†‚Äö√†√® subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-IL3RA antibody (Alexa-fluor 647)
STJ170011	St John's Laboratory	100 µg	EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ‚Äö√†√∂‚àö√¢‚Äö√¢√†‚Äö√†√® subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-CD207 antibody (Alexa-fluor 488)
STJ170014	St John's Laboratory	100 µg	EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-CD207 antibody (Alexa-fluor 546)
STJ170015	St John's Laboratory	100 µg	EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-CD207 antibody (Alexa-fluor 647)
STJ170016	St John's Laboratory	100 µg	EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-IL7R antibody (Alexa-fluor 488)
STJ170020	St John's Laboratory	100 µg	EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, na‚Äö√†√∂‚àö√¢¬¨¬®‚àö√≤ve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-IL7R antibody (Alexa-fluor 546)
STJ170021	St John's Laboratory	100 µg	EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, na‚Äö√†√∂‚àö√¢¬¨¬®‚àö√≤ve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-IL7R antibody (Alexa-fluor 647)
STJ170022	St John's Laboratory	100 µg	EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, na‚Äö√†√∂‚àö√¢¬¨¬®‚àö√≤ve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Goat anti Mouse IgG1 (Alexa Fluor 488)
43R-1649	Fitzgerald	500 ug	EUR 570
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488)

Anti-Hu CD16 Alexa Fluor® 488
A4-646-T100	ExBio	100 tests	EUR 269

Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG)
50337	Alpha Diagnostics	0.5 ml	EUR 225

Alpha Fluor™ 594 C5 Maleimide
1891	AAT Bioquest	1 mg	EUR 219
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12171501

Streptavidin-Alexa594 (Alexas fluor 594) conjugate
SV-A594-100	Alpha Diagnostics	100 tests	EUR 225

Alpha Fluor™ 532 acid [equivalent to Alexa Fluor™ 532 acid]
1795	AAT Bioquest	10 mg	EUR 393
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12171501

AF350 Phalloidin [equivalent to Alexa Fluor® 350 phalloidin]
23150	AAT Bioquest	300 Tests	EUR 306
R-phrase: R23, R24, R25 H-Phrase: H301, H311, H331 Symbol for dangerous compounds: T UNSPEC Code: 12352200

AF488 Phalloidin [equivalent to Alexa Fluor® 488 phalloidin]
23153	AAT Bioquest	300 Tests	EUR 306
R-phrase: R23, R24, R25 H-Phrase: H301, H311, H331 Symbol for dangerous compounds: T UNSPEC Code: 12352200

AF350-streptavidin conjugate [Streptavidin, Alexa Fluor™ 350 Conjugate]
16890	AAT Bioquest	1 mg	EUR 176
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12171501

AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate]
16891	AAT Bioquest	1 mg	EUR 176
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12171501

Donkey anti Goat IgG (H + L) (Alexa Fluor 647)
43R-ID028AF	Fitzgerald	500 ug	EUR 430
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 647)
43R-ID060AF	Fitzgerald	300 ug	EUR 425
Description: Donkey anti Chicken IgY (H + L) (Fab'2) (Alexa Fluor 647)

Anti-Hu CD72 Alexa Fluor^® 488
A4-310-T100	ExBio	100 tests	EUR 269

Anti-Bov CD9 Alexa Fluor^® 488
A4-354-C100	ExBio	0.1 mg	EUR 269

Anti-Hu CD30 Alexa Fluor^® 700
A7-455-T100	ExBio	100 tests	EUR 269

Anti-Hu CD94 Alexa Fluor^® 700
A7-727-T100	ExBio	100 tests	EUR 269

Anti-Hu CD56 Alexa Fluor^® 700
A7-789-T100	ExBio	100 tests	EUR 269

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 647–conjugated
S0013	Affbiotech	200ul	EUR 304

Goat Anti-Mouse IgG(H+L) Alexa Fluor 647–conjugated
S0014	Affbiotech	200ul	EUR 304

Goat Anti-Mouse IgG(H+L) Alexa Fluor 488–conjugated
S0017	Affbiotech	200ul	EUR 304

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488–conjugated
S0018	Affbiotech	200ul	EUR 304

Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated
Ab8032-001	GenDepot	0.5mg	EUR 435

Anti-Hu CD3 zeta (pY153) Alexa Fluor^® 488
A4-686-C100	ExBio	0.1 mg	EUR 269

Anti-Hu CD3 zeta (pY72) Alexa Fluor^® 488
A4-712-C100	ExBio	0.1 mg	EUR 269

Anti-Hu CD3 zeta (pY142) Alexa Fluor^® 488
A4-730-C100	ExBio	0.1 mg	EUR 269

Anti-Hu CD3 zeta (pY111) Alexa Fluor^® 488
A4-737-C100	ExBio	0.1 mg	EUR 269

Anti-Hu CD3 zeta (pY153) Alexa Fluor^® 647
A6-686-C100	ExBio	0.1 mg	EUR 269

Anti-Hu CD3 zeta (pY72) Alexa Fluor^® 647
A6-712-C100	ExBio	0.1 mg	EUR 269

Anti-Hu CD3 zeta (pY142) Alexa Fluor^® 647
A6-730-C100	ExBio	0.1 mg	EUR 269

Anti-Hu CD3 zeta (pY111) Alexa Fluor^® 647
A6-737-C100	ExBio	0.1 mg	EUR 269

Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor^® 488)
M09406	BosterBio	100ug	EUR 565
Description: Mouse Monoclonal LAMP3 (human) Antibody (104G4) (Alexa Fluor^® 488). Validated in IHC and tested in Human.

Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor^® 488)
M02316	BosterBio	100ug	EUR 580
Description: Mouse Monoclonal Langerin (human) Antibody (DCGM4/122D5) (Alexa Fluor^® 488). Validated in IHC and tested in Human.

Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG)
50336	Alpha Diagnostics	0.5 ml	EUR 225

Recombinant (E.Coli) Human Thyrostimulin Beta
RP-594	Alpha Diagnostics	10 ug	EUR 286

BBT-594
B2114-25	Biovision		EUR 631

BBT-594
B2114-5	Biovision		EUR 196

Alpha Fluor™ 488 amine
1705	AAT Bioquest	1 mg	EUR 306
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12171501

Alpha Fluor™ 488 Hydroxylamine
1900	AAT Bioquest	1 mg	EUR 306
R-phrase: R20, R21, R22 H-Phrase: H303, H313, H333 Symbol for dangerous compounds: Xn UNSPEC Code: 12171501

Tide Fluor 2-LL-37
H-8286.0100	Bachem	0.1mg	EUR 312
Description: Sum Formula: C205H340N60O53+dye

Tide Fluor 2-LL-37
H-8286.0500	Bachem	0.5mg	EUR 1017
Description: Sum Formula: C205H340N60O53+dye

Anti-Cytokeratins Alexa Fluor488
A4-108-C025	ExBio	0.025 mg	EUR 175

Anti-Cytokeratins Alexa Fluor488
A4-108-C100	ExBio	0.1 mg	EUR 310

Anti-PSMA Alexa Fluor488
A4-539-C025	ExBio	0.025 mg	EUR 227

Anti-PSMA Alexa Fluor488
A4-539-C100	ExBio	0.1 mg	EUR 414

Anti-FoxP3 Alexa Fluor488
A4-601-C025	ExBio	0.025 mg	EUR 201

Anti-FoxP3 Alexa Fluor488
A4-601-C100	ExBio	0.1 mg	EUR 362

Anti-Phosphotyrosine Alexa Fluor647
A6-263-C025	ExBio	0.025 mg	EUR 154

Anti-Phosphotyrosine Alexa Fluor647
A6-263-C100	ExBio	0.1 mg	EUR 269

Anti-LCK Alexa Fluor647
A6-269-C025	ExBio	0.025 mg	EUR 206

Anti-LCK Alexa Fluor647
A6-269-C100	ExBio	0.1 mg	EUR 373

Anti-FoxP3 Alexa Fluor647
A6-601-C025	ExBio	0.025 mg	EUR 201

Anti-FoxP3 Alexa Fluor647
A6-601-C100	ExBio	0.1 mg	EUR 362

Multiplex PCR for simultaneous identification of the most typical European Opisthorchiid and Heterophyid in fish or fish merchandise

Amongst others, the households Opisthorchiidae and Heterophyidae contains a number of genera inflicting fish-borne zoonoses and distributed additionally in European Nations and which can be included within the ParaFishControl (Superior Instruments and Analysis Methods for Parasite Management in European farmed fish) H2020 EU venture.

Because of the small dimension of the metacercariae, the infective stage for human, these parasites can’t be detected visually in fish and monitoring requires professional utility of time-consuming methods. The goal of this was to develop a fast and inexpensive molecular technique primarily based on multiplex PCR for simultaneous identification of metacercariae of the most typical European Opisthorchiid and Heterophyid in fish or fish merchandise.

Key phrases: Europe; Fish merchandise; Freshwater fish; Heterophyidae; Multiplex PCR; Opisthorchiidae

TaqMan probe primarily based multiplex quantitative PCR assay for dedication of bovine, porcine and fish DNA in gelatin admixture, meals merchandise and dietary dietary supplements.

Detection of animal supplies in gelatin-based merchandise is required to deal with spiritual and cultural considerations, as a result of porcine and bovine gelatins are prohibited in Halal, Kosher and Hindus shopper goods. On this paper, multiplex quantitative polymerase chain response (qPCR) assay utilizing TaqMan probe was developed to discriminate bovine, porcine and fish gelatin species in a single assay platform.

The assay was particular to cattle, pigs and fish, having been examined in opposition to 14 non-target species. The restrict of detection, underneath gelatin admixed circumstances, was 0.005 ng/µL. Lastly, a pilot survey was undertaken testing 35 Halal branded processed meals and dietary gadgets. Out of 35 samples, solely two had been discovered to be optimistic for porcine species. The authenticity of those two qPCR merchandise was confirmed by DNA sequencing evaluation, which confirmed 99-100% similarity with Sus scrofa (Wild boar) species.

hsa-mir-521 Real-Time RT-PCR Detection and U6 Calibration Kit
20-abx097601	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

0,1ML THIN WALL TUBES 8 PER STRIP. CLEAR & REAL TIME STRIP CAPS
PCR-0108-LP-RT-C	CORNING	125/pk	EUR 709
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

0,1ML THIN WALL TUBES 8 PER STRIP. WHITE & REAL TIME STRIP CAPS
PCR-0108-LP-RT-W	CORNING	125/pk	EUR 770
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

dsGreen for Real-Time PCR, 100×, 100 uL
11010	lumiprobe	100 uL	EUR 59

dsGreen for Real-Time PCR, 100×, 2 mL
61010	lumiprobe	2 mL	EUR 363

dsGreen for Real-Time PCR, 100×, 1.5 mL
51010	lumiprobe	1.5 mL	EUR 331

dsGreen for Real-Time PCR, 100×, 1 mL
41010	lumiprobe	1 mL	EUR 249

dsGreen for Real-Time PCR, 100×, 10 mL
81010	lumiprobe	10 mL	EUR 869

dsGreen for Real-Time PCR, 100×, 5 mL
71010	lumiprobe	5 mL	EUR 625

dsGreen for Real-Time PCR, 100×, 50 mL
91010	lumiprobe	50 mL	EUR 3169

hsa-let-7a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097603	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-let-7b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097604	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-let-7c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097605	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-let-7d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097606	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-let-7e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097607	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-let-7f Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097608	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-let-7g Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097609	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-let-7i Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097610	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-let-7f Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097611	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-1 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097612	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-7 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097613	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-9 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097614	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-9* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097615	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-10a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097616	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-10b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097617	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-10a* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097618	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-15a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097619	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-15b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097620	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-16 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097622	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-18a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097625	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-18b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097626	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-19a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097627	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-19b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097628	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-20a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097629	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-20b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097630	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-21 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097631	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-22 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097632	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-23a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097633	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-23b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097634	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-25 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097636	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-26a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097637	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-26b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097638	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-27a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097639	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-27b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097640	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-29a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097642	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-29b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097643	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-29c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097644	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-30a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097645	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-30b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097647	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-30c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097648	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-30d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097650	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-30e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097651	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-31 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097652	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-32 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097653	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-33a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097654	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-34a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097657	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-34b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097659	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-92a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097661	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-92b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097662	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-93 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097663	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-95 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097664	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-96 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097665	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-98 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097666	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-99a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097667	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-99b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097669	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-100 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097671	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-101 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097673	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-103 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097675	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-105 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097676	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-106a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097677	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-106b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097678	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-107 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097679	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-124 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097680	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-125a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097681	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-127 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097686	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-129 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097689	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-130a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097690	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-130b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097691	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-132 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097692	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-133a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097693	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-133b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097694	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-134 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097695	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-135b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097696	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-137 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097699	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-138 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097700	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-139 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097701	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-140 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097702	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-141 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097703	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-143 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097706	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-145 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097708	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

hsa-mir-146a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097709	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
Shipped within 3 weeks.

Excessive-efficiency transformation of archaea by direct PCR merchandise with its utility to directed evolution of a thermostable enzyme

Screening of business meat merchandise from grocery store chains for feline derivatives utilizing SP-PCR-RLFP and lab-on-a-chip

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 594)

Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594)

Endoglin/CD105 Alexa Fluor

Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 594–conjugated

Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594)

Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated

EGFR antibody (Alexa Fluor 488)

SAM FCM (Alexa Fluor 647)

SAM FCM (Alexa Fluor 488)

Anti-RPSA Alexa Fluor® 488

Anti-CD40 antibody (Alexa-fluor 488)

Anti-LAMP3 antibody (Alexa-fluor 488)

Anti-LAMP3 antibody (Alexa-fluor 546)

Anti-LAMP3 antibody (Alexa-fluor 647)

Anti-IL3RA antibody (Alexa-fluor 488)

Anti-IL3RA antibody (Alexa-fluor 546)

Anti-IL3RA antibody (Alexa-fluor 647)

Anti-CD207 antibody (Alexa-fluor 488)

Anti-CD207 antibody (Alexa-fluor 546)

Anti-CD207 antibody (Alexa-fluor 647)

Anti-IL7R antibody (Alexa-fluor 488)

Anti-IL7R antibody (Alexa-fluor 546)

Anti-IL7R antibody (Alexa-fluor 647)

Goat anti Mouse IgG1 (Alexa Fluor 488)

Anti-Hu CD16 Alexa Fluor® 488

Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG)

Alpha Fluor™ 594 C5 Maleimide

Streptavidin-Alexa594 (Alexas fluor 594) conjugate

Alpha Fluor™ 532 acid [equivalent to Alexa Fluor™ 532 acid]

AF350 Phalloidin [equivalent to Alexa Fluor® 350 phalloidin]

AF488 Phalloidin [equivalent to Alexa Fluor® 488 phalloidin]

AF350-streptavidin conjugate [Streptavidin, Alexa Fluor™ 350 Conjugate]

AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate]

Donkey anti Goat IgG (H + L) (Alexa Fluor 647)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 647)

Anti-Hu CD72 Alexa Fluor® 488

Anti-Bov CD9 Alexa Fluor® 488

Anti-Hu CD30 Alexa Fluor® 700

Anti-Hu CD94 Alexa Fluor® 700

Anti-Hu CD56 Alexa Fluor® 700

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 647–conjugated

Goat Anti-Mouse IgG(H+L) Alexa Fluor 647–conjugated

Goat Anti-Mouse IgG(H+L) Alexa Fluor 488–conjugated

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488–conjugated

Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 488

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 488

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 488

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 488

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 647

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 647

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 647

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 647

Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor® 488)

Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor® 488)

Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG)

Recombinant (E.Coli) Human Thyrostimulin Beta

BBT-594

BBT-594

Alpha Fluor™ 488 amine

Alpha Fluor™ 488 Hydroxylamine

Tide Fluor 2-LL-37

Tide Fluor 2-LL-37

Anti-Cytokeratins Alexa Fluor488

Anti-Cytokeratins Alexa Fluor488

Anti-PSMA Alexa Fluor488

Anti-PSMA Alexa Fluor488

Anti-FoxP3 Alexa Fluor488

Anti-FoxP3 Alexa Fluor488

Anti-Phosphotyrosine Alexa Fluor647

Anti-Phosphotyrosine Alexa Fluor647

Anti-LCK Alexa Fluor647

Anti-LCK Alexa Fluor647

Anti-FoxP3 Alexa Fluor647

Anti-FoxP3 Alexa Fluor647

hsa-mir-521 Real-Time RT-PCR Detection and U6 Calibration Kit

Anti-Hu CD72 Alexa Fluor^® 488

Anti-Bov CD9 Alexa Fluor^® 488

Anti-Hu CD30 Alexa Fluor^® 700

Anti-Hu CD94 Alexa Fluor^® 700

Anti-Hu CD56 Alexa Fluor^® 700

Anti-Hu CD3 zeta (pY153) Alexa Fluor^® 488

Anti-Hu CD3 zeta (pY72) Alexa Fluor^® 488

Anti-Hu CD3 zeta (pY142) Alexa Fluor^® 488

Anti-Hu CD3 zeta (pY111) Alexa Fluor^® 488

Anti-Hu CD3 zeta (pY153) Alexa Fluor^® 647

Anti-Hu CD3 zeta (pY72) Alexa Fluor^® 647

Anti-Hu CD3 zeta (pY142) Alexa Fluor^® 647

Anti-Hu CD3 zeta (pY111) Alexa Fluor^® 647

Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor^® 488)

Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor^® 488)