Excessive-efficiency transformation of archaea by direct PCR merchandise with its utility to directed evolution of a thermostable enzyme
Hyperthermophilic archaea with distinctive biochemical and physiological traits are necessary organisms for basic analysis of life science and have nice potential for biotechnological functions. Nonetheless, low transformation effectivity of international DNA molecules impedes developments in genetic modification instruments and industrial functions.
- On this research, we utilized extended overlap extension PCR (POE-PCR) to generate multimeric DNA molecules after which remodeled them into two hyperthermophilic archaea, Thermococcus kodakarensis KOD1 and Pyrococcus yayanosii A1.
- This research was the primary instance to display the improved transformation efficiencies of POE-PCR merchandise by an element of roughly 100 for T. kodakarensis KOD1 and eight for P. yayanosii A1, respectively, relative to round shuttle plasmids.
- Moreover, directed evolution of a modestly thermophilic enzyme, Methanothermococcus okinawensis 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), was carried out to acquire extra steady ones on account of excessive transformation effectivity of T. kodakarensis (i.e. ~3 × 104 CFU per μg DNA). T. kodakarensis harbouring essentially the most thermostable MoHMGR mutant can develop within the presence of a thermostable antibiotic simvastatin at 85°C and even greater temperatures.
- This excessive transformation effectivity method couldn’t solely assist develop extra hyperthermophilic enzyme mutants by way of directed evolution but in addition simplify genetical modification of archaea, which could possibly be novel hosts for industrial biotechnology
Screening of business meat merchandise from grocery store chains for feline derivatives utilizing SP-PCR-RLFP and lab-on-a-chip
Willpower of feline meat in meals merchandise is a vital situation for social, well being, financial and spiritual concern. Therefore this paper documented the applying of species particular polymerase chain reaction-restriction fragment size polymorphism (SP-PCR-RFLP) assay concentrating on a short-fragments (69 bp) of mitochondrial cytochrome b (cytb) gene to display feline meat in industrial meat merchandise utilizing lab-on-a-chip.
The SP-PCR assay proved its specificity theoretically and experimentally whereas testing with totally different widespread animal, aquatic and plant species of DNA. The feline particular (69 bp, 43- and 26-bp) attribute molecular DNA sample was noticed by SP-PCR and RFLP evaluation.
For assay efficiency, it was examined in three several types of industrial dummy meat merchandise equivalent to frankfurters, nuggets and meatballs and digested with AluI-restriction enzyme. The best sensitivity of the assay utilizing lab-on-a-chip was as little as 0.1 pg or 0.01 % (w/w) in industrial dummy meat merchandise. Now we have additionally utilized this assay to display three necessary industrial meat merchandise of six totally different model from six grocery store chains positioned at three totally different states of Malaysia.
Thus whole 378 samples had been examined to validate the specificity, sensitivity, stability of the assay and utilization of it for industrial meat product screening.
Key phrases:Â Industrial meat merchandise; Feline meat; Halal; Lab-on-a-chip; Restriction digestion.
genpcr
 Alexa Fluor 594–conjugated) Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated |
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| S0005 | Affbiotech | 200ul | EUR 376 |
 Alexa Fluor 594–conjugated) Goat Anti-Rabbit IgG(H+L) Alexa Fluor 594–conjugated |
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| S0006 | Affbiotech | 200ul | EUR 376 |
) EGFR antibody (Alexa Fluor 488) |
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| 61R-E109BAF | Fitzgerald | 125 ug | EUR 706 |
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Description: Mouse monoclonal EGFR antibody (Alexa Fluor 488) |
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) SAM FCM (Alexa Fluor 647) |
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| abx098902-100tests | Abbexa | 100 tests | EUR 1233 |
) SAM FCM (Alexa Fluor 488) |
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| abx098904-60tests | Abbexa | 60 tests | EUR 1358 |
 (Fab 2) (Alexa Fluor 594)) Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594) |
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| 43R-ID012AF | Fitzgerald | 300 ug | EUR 410 |
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Description: Donkey anti Goat IgG (H + L) secondary antibody (Fab'2) (Alexa Fluor 594) |
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, Alexa Fluor® 594 Conjugated) Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated |
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| Ab8011-001 | GenDepot | 1mg | EUR 334 |
 Anti-RPSA Alexa Fluor® 488 |
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| A4-829-C100 | ExBio | 0.1 mg | EUR 310 |
) Anti-CD40 antibody (Alexa-fluor 488) |
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| STJ170000 | St John's Laboratory | 100 µg | EUR 393 |
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Description: CD40 (48 to 50 kDa) is a transmembrane glycoprotein mainly expressed on the surface of B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 is the receptor for CD40 ligand. CD40L (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40, predominantly on activated CD4+ T cells. Following interaction with CD40 ligand, CD40 mediates a number of major immunoregulatory functions, central to the control of thymus dependent humoral immunity and may be critical in the development of cell mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin |
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) Anti-LAMP3 antibody (Alexa-fluor 488) |
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| STJ170004 | St John's Laboratory | 100 µg | EUR 393 |
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Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface. |
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) Anti-LAMP3 antibody (Alexa-fluor 546) |
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| STJ170005 | St John's Laboratory | 100 µg | EUR 393 |
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Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface. |
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) Anti-LAMP3 antibody (Alexa-fluor 647) |
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| STJ170006 | St John's Laboratory | 100 µg | EUR 393 |
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Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface. |
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) Anti-IL3RA antibody (Alexa-fluor 488) |
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| STJ170009 | St John's Laboratory | 100 µg | EUR 393 |
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Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ‚Äö√†√∂‚à ö√¢‚Äö√¢√†‚Äö√†√® subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment |
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) Anti-IL3RA antibody (Alexa-fluor 546) |
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| STJ170010 | St John's Laboratory | 100 µg | EUR 393 |
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Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ‚Äö√†√∂‚à ö√¢‚Äö√¢√†‚Äö√†√® subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment |
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) Anti-IL3RA antibody (Alexa-fluor 647) |
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| STJ170011 | St John's Laboratory | 100 µg | EUR 393 |
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Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ‚Äö√†√∂‚à ö√¢‚Äö√¢√†‚Äö√†√® subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment |
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) Anti-CD207 antibody (Alexa-fluor 488) |
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| STJ170014 | St John's Laboratory | 100 µg | EUR 393 |
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Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307) |
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) Anti-CD207 antibody (Alexa-fluor 546) |
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| STJ170015 | St John's Laboratory | 100 µg | EUR 393 |
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Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307) |
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) Anti-CD207 antibody (Alexa-fluor 647) |
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| STJ170016 | St John's Laboratory | 100 µg | EUR 393 |
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Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307) |
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) Anti-IL7R antibody (Alexa-fluor 488) |
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| STJ170020 | St John's Laboratory | 100 µg | EUR 393 |
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Description: The IL7-R consists of 2 chains, IL-7R |
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) Anti-IL7R antibody (Alexa-fluor 546) |
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| STJ170021 | St John's Laboratory | 100 µg | EUR 393 |
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Description: The IL7-R consists of 2 chains, IL-7R |
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) Anti-IL7R antibody (Alexa-fluor 647) |
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| STJ170022 | St John's Laboratory | 100 µg | EUR 393 |
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Description: The IL7-R consists of 2 chains, IL-7R |
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) Goat anti Mouse IgG1 (Alexa Fluor 488) |
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| 43R-1649 | Fitzgerald | 500 ug | EUR 570 |
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Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488) |
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 Anti-Hu CD16 Alexa Fluor® 488 |
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| A4-646-T100 | ExBio | 100 tests | EUR 269 |
-Alexa 594 Fluor conjugate (adsorbed with human IgG)) Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG) |
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| 50337 | Alpha Diagnostics | 0.5 ml | EUR 225 |
 Alpha Fluor™ 594 C5 Maleimide |
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| 1891 | AAT Bioquest | 1 mg | EUR 219 |
 conjugate) Streptavidin-Alexa594 (Alexas fluor 594) conjugate |
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| SV-A594-100 | Alpha Diagnostics | 100 tests | EUR 225 |
 Alpha Fluor™ 532 acid [equivalent to Alexa Fluor™ 532 acid] |
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| 1795 | AAT Bioquest | 10 mg | EUR 393 |
 (Alexa Fluor 647)) Donkey anti Goat IgG (H + L) (Alexa Fluor 647) |
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| 43R-ID028AF | Fitzgerald | 500 ug | EUR 430 |
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Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647) |
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 (Alexa Fluor 647)) Donkey anti Chicken IgY (H + L) (Alexa Fluor 647) |
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| 43R-ID060AF | Fitzgerald | 300 ug | EUR 425 |
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Description: Donkey anti Chicken IgY (H + L) (Fab'2) (Alexa Fluor 647) |
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 AF350-streptavidin conjugate [Streptavidin, Alexa Fluor™ 350 Conjugate] |
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| 16890 | AAT Bioquest | 1 mg | EUR 176 |
 AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate] |
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| 16891 | AAT Bioquest | 1 mg | EUR 176 |
 AF350 Phalloidin [equivalent to Alexa Fluor® 350 phalloidin] |
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| 23150 | AAT Bioquest | 300 Tests | EUR 306 |
 AF488 Phalloidin [equivalent to Alexa Fluor® 488 phalloidin] |
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| 23153 | AAT Bioquest | 300 Tests | EUR 306 |
 Anti-Hu CD72 Alexa Fluor® 488 |
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| A4-310-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Bov CD9 Alexa Fluor® 488 |
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| A4-354-C100 | ExBio | 0.1 mg | EUR 269 |
 Anti-Hu CD30 Alexa Fluor® 700 |
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| A7-455-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD94 Alexa Fluor® 700 |
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| A7-727-T100 | ExBio | 100 tests | EUR 269 |
 Anti-Hu CD56 Alexa Fluor® 700 |
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| A7-789-T100 | ExBio | 100 tests | EUR 269 |
 Alexa Fluor 647–conjugated) Goat Anti-Rabbit IgG(H+L) Alexa Fluor 647–conjugated |
|||
| S0013 | Affbiotech | 200ul | EUR 304 |
 Alexa Fluor 647–conjugated) Goat Anti-Mouse IgG(H+L) Alexa Fluor 647–conjugated |
|||
| S0014 | Affbiotech | 200ul | EUR 304 |
 Alexa Fluor 488–conjugated) Goat Anti-Mouse IgG(H+L) Alexa Fluor 488–conjugated |
|||
| S0017 | Affbiotech | 200ul | EUR 304 |
 Alexa Fluor 488–conjugated) Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488–conjugated |
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| S0018 | Affbiotech | 200ul | EUR 304 |
 Alexa Fluor<sup>®</sup> 488) Anti-Hu CD3 zeta (pY153) Alexa Fluor® 488 |
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| A4-686-C100 | ExBio | 0.1 mg | EUR 269 |
 Alexa Fluor<sup>®</sup> 488) Anti-Hu CD3 zeta (pY72) Alexa Fluor® 488 |
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| A4-712-C100 | ExBio | 0.1 mg | EUR 269 |
 Alexa Fluor<sup>®</sup> 488) Anti-Hu CD3 zeta (pY142) Alexa Fluor® 488 |
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| A4-730-C100 | ExBio | 0.1 mg | EUR 269 |
 Alexa Fluor<sup>®</sup> 488) Anti-Hu CD3 zeta (pY111) Alexa Fluor® 488 |
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| A4-737-C100 | ExBio | 0.1 mg | EUR 269 |
 Alexa Fluor<sup>®</sup> 647) Anti-Hu CD3 zeta (pY153) Alexa Fluor® 647 |
|||
| A6-686-C100 | ExBio | 0.1 mg | EUR 269 |
 Alexa Fluor<sup>®</sup> 647) Anti-Hu CD3 zeta (pY72) Alexa Fluor® 647 |
|||
| A6-712-C100 | ExBio | 0.1 mg | EUR 269 |
 Alexa Fluor<sup>®</sup> 647) Anti-Hu CD3 zeta (pY142) Alexa Fluor® 647 |
|||
| A6-730-C100 | ExBio | 0.1 mg | EUR 269 |
 Alexa Fluor<sup>®</sup> 647) Anti-Hu CD3 zeta (pY111) Alexa Fluor® 647 |
|||
| A6-737-C100 | ExBio | 0.1 mg | EUR 269 |
, Alexa Fluor® 488 Conjugated) Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated |
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| Ab8032-001 | GenDepot | 0.5mg | EUR 435 |
 Monoclonal Antibody (104G4) (Alexa Fluor<sup>®</sup> 488)) Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor® 488) |
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| M09406 | BosterBio | 100ug | EUR 565 |
|
Description: Mouse Monoclonal LAMP3 (human) Antibody (104G4) (Alexa Fluor® 488). Validated in IHC and tested in Human. |
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 Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor<sup>®</sup> 488)) Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor® 488) |
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| M02316 | BosterBio | 100ug | EUR 580 |
|
Description: Mouse Monoclonal Langerin (human) Antibody (DCGM4/122D5) (Alexa Fluor® 488). Validated in IHC and tested in Human. |
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-Alexa 488 Fluor conjugate (adsorbed with human IgG)) Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG) |
|||
| 50336 | Alpha Diagnostics | 0.5 ml | EUR 225 |
 Human Thyrostimulin Beta) Recombinant (E.Coli) Human Thyrostimulin Beta |
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| RP-594 | Alpha Diagnostics | 10 ug | EUR 286 |
 Anti-Cytokeratins Alexa Fluor488 |
|||
| A4-108-C025 | ExBio | 0.025 mg | EUR 175 |
Anti-Cytokeratins Alexa Fluor488 |
|||
| A4-108-C100 | ExBio | 0.1 mg | EUR 310 |
 Anti-PSMA Alexa Fluor488 |
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| A4-539-C025 | ExBio | 0.025 mg | EUR 227 |
Anti-PSMA Alexa Fluor488 |
|||
| A4-539-C100 | ExBio | 0.1 mg | EUR 414 |
 Anti-FoxP3 Alexa Fluor488 |
|||
| A4-601-C025 | ExBio | 0.025 mg | EUR 201 |
Anti-FoxP3 Alexa Fluor488 |
|||
| A4-601-C100 | ExBio | 0.1 mg | EUR 362 |
 Anti-Phosphotyrosine Alexa Fluor647 |
|||
| A6-263-C025 | ExBio | 0.025 mg | EUR 154 |
Anti-Phosphotyrosine Alexa Fluor647 |
|||
| A6-263-C100 | ExBio | 0.1 mg | EUR 269 |
 Anti-LCK Alexa Fluor647 |
|||
| A6-269-C025 | ExBio | 0.025 mg | EUR 206 |
Anti-LCK Alexa Fluor647 |
|||
| A6-269-C100 | ExBio | 0.1 mg | EUR 373 |
 Anti-FoxP3 Alexa Fluor647 |
|||
| A6-601-C025 | ExBio | 0.025 mg | EUR 201 |
Anti-FoxP3 Alexa Fluor647 |
|||
| A6-601-C100 | ExBio | 0.1 mg | EUR 362 |
 Alpha Fluor™ 488 amine |
|||
| 1705 | AAT Bioquest | 1 mg | EUR 306 |
 Alpha Fluor™ 488 Hydroxylamine |
|||
| 1900 | AAT Bioquest | 1 mg | EUR 306 |
 Tide Fluor 2-LL-37 |
|||
| H-8286.0100 | Bachem | 0.1mg | EUR 312 |
|
Description: Sum Formula: C205H340N60O53+dye |
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Tide Fluor 2-LL-37 |
|||
| H-8286.0500 | Bachem | 0.5mg | EUR 1017 |
|
Description: Sum Formula: C205H340N60O53+dye |
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 BBT-594 |
|||
| B2114-25 | Biovision | EUR 631 | |
BBT-594 |
|||
| B2114-5 | Biovision | EUR 196 | |
 Anti-Cytokeratin 18 Alexa Fluor488 |
|||
| A4-106-C025 | ExBio | 0.025 mg | EUR 186 |
Anti-Cytokeratin 18 Alexa Fluor488 |
|||
| A4-106-C100 | ExBio | 0.1 mg | EUR 331 |
 Anti-Cytokeratin 19 Alexa Fluor488 |
|||
| A4-120-C025 | ExBio | 0.025 mg | EUR 186 |
Anti-Cytokeratin 19 Alexa Fluor488 |
|||
| A4-120-C100 | ExBio | 0.1 mg | EUR 331 |
Multiplex PCR for simultaneous identification of the most typical European Opisthorchiid and Heterophyid in fish or fish merchandise
Amongst others, the households Opisthorchiidae and Heterophyidae contains a number of genera inflicting fish-borne zoonoses and distributed additionally in European Nations and which can be included within the ParaFishControl (Superior Instruments and Analysis Methods for Parasite Management in European farmed fish) H2020 EU venture.
Because of the small dimension of the metacercariae, the infective stage for human, these parasites can’t be detected visually in fish and monitoring requires professional utility of time-consuming methods. The goal of this was to develop a fast and inexpensive molecular technique primarily based on multiplex PCR for simultaneous identification of metacercariae of the most typical European Opisthorchiid and Heterophyid in fish or fish merchandise.
Key phrases:Â Europe; Fish merchandise; Freshwater fish; Heterophyidae; Multiplex PCR; Opisthorchiidae
TaqMan probe primarily based multiplex quantitative PCR assay for dedication of bovine, porcine and fish DNA in gelatin admixture, meals merchandise and dietary dietary supplements.
Detection of animal supplies in gelatin-based merchandise is required to deal with spiritual and cultural considerations, as a result of porcine and bovine gelatins are prohibited in Halal, Kosher and Hindus shopper goods. On this paper, multiplex quantitative polymerase chain response (qPCR) assay utilizing TaqMan probe was developed to discriminate bovine, porcine and fish gelatin species in a single assay platform.
The assay was particular to cattle, pigs and fish, having been examined in opposition to 14 non-target species. The restrict of detection, underneath gelatin admixed circumstances, was 0.005 ng/µL. Lastly, a pilot survey was undertaken testing 35 Halal branded processed meals and dietary gadgets. Out of 35 samples, solely two had been discovered to be optimistic for porcine species. The authenticity of those two qPCR merchandise was confirmed by DNA sequencing evaluation, which confirmed 99-100% similarity with Sus scrofa (Wild boar) species.
) PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA) |
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| 31033 | Biotium | 1kit | EUR 428 |
|
Description: Minimum order quantity: 1 unit of 1kit |
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) PMA Real-Time PCR Bacterial Viability Kit - Mycobacterium tuberculosis (groEL2) |
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| 31034 | Biotium | 1kit | EUR 399 |
|
Description: Minimum order quantity: 1 unit of 1kit |
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) PMA Real-Time PCR Bacterial Viability Kit - Staphylococcus aureus (nuc) |
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| 31035 | Biotium | 1kit | EUR 399 |
|
Description: Minimum order quantity: 1 unit of 1kit |
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) PMA Real-Time PCR Bacterial Viability Kit - Staphylococcus aureus (mecA) |
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| 31036 | Biotium | 1kit | EUR 399 |
|
Description: Minimum order quantity: 1 unit of 1kit |
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) PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA) |
|||
| 31050 | Biotium | 1kit | EUR 428 |
|
Description: Minimum order quantity: 1 unit of 1kit |
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) PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly) |
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| 31051 | Biotium | 1kit | EUR 399 |
|
Description: Minimum order quantity: 1 unit of 1kit |
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) PMA Real-Time PCR Bacterial Viability Kit - Legionella pneumophila (mip) |
|||
| 31053 | Biotium | 1kit | EUR 428 |
|
Description: Minimum order quantity: 1 unit of 1kit |
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 hsa-let-7a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097603 | Abbexa |
|
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 hsa-let-7b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097604 | Abbexa |
|
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 hsa-let-7c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097605 | Abbexa |
|
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 hsa-let-7d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097606 | Abbexa |
|
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 hsa-let-7e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097607 | Abbexa |
|
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 hsa-let-7f Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097608 | Abbexa |
|
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 hsa-let-7g Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097609 | Abbexa |
|
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 hsa-let-7i Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097610 | Abbexa |
|
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hsa-let-7f Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097611 | Abbexa |
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 hsa-mir-1 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097612 | Abbexa |
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 hsa-mir-7 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097613 | Abbexa |
|
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 hsa-mir-9 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097614 | Abbexa |
|
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 hsa-mir-9* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097615 | Abbexa |
|
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 hsa-mir-10a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097616 | Abbexa |
|
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 hsa-mir-10b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097617 | Abbexa |
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 hsa-mir-10a* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097618 | Abbexa |
|
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 hsa-mir-15a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097619 | Abbexa |
|
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 hsa-mir-15b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097620 | Abbexa |
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 hsa-mir-16 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097622 | Abbexa |
|
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 hsa-mir-18a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097625 | Abbexa |
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 hsa-mir-18b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097626 | Abbexa |
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 hsa-mir-19a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097627 | Abbexa |
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 hsa-mir-19b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097628 | Abbexa |
|
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 hsa-mir-20a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097629 | Abbexa |
|
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 hsa-mir-20b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097630 | Abbexa |
|
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 hsa-mir-21 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
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| 20-abx097631 | Abbexa |
|
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 hsa-mir-22 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097632 | Abbexa |
|
|
 hsa-mir-23a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097633 | Abbexa |
|
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 hsa-mir-23b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097634 | Abbexa |
|
|
 hsa-mir-25 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097636 | Abbexa |
|
|
 hsa-mir-26a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097637 | Abbexa |
|
|
 hsa-mir-26b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097638 | Abbexa |
|
|
 hsa-mir-27a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097639 | Abbexa |
|
|
 hsa-mir-27b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097640 | Abbexa |
|
|
 hsa-mir-29a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097642 | Abbexa |
|
|
 hsa-mir-29b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097643 | Abbexa |
|
|
 hsa-mir-29c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097644 | Abbexa |
|
|
 hsa-mir-30a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097645 | Abbexa |
|
|
 hsa-mir-30b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097647 | Abbexa |
|
|
 hsa-mir-30c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097648 | Abbexa |
|
|
 hsa-mir-30d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097650 | Abbexa |
|
|
 hsa-mir-30e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097651 | Abbexa |
|
|
 hsa-mir-31 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097652 | Abbexa |
|
|
 hsa-mir-32 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097653 | Abbexa |
|
|
 hsa-mir-33a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097654 | Abbexa |
|
|
 hsa-mir-34a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097657 | Abbexa |
|
|
 hsa-mir-34b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097659 | Abbexa |
|
|
 hsa-mir-92a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097661 | Abbexa |
|
|
 hsa-mir-92b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097662 | Abbexa |
|
|
 hsa-mir-93 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097663 | Abbexa |
|
|
 hsa-mir-95 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097664 | Abbexa |
|
|
 hsa-mir-96 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097665 | Abbexa |
|
|
 hsa-mir-98 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097666 | Abbexa |
|
|
 hsa-mir-99a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097667 | Abbexa |
|
|
 hsa-mir-99b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097669 | Abbexa |
|
|
 hsa-mir-100 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097671 | Abbexa |
|
|
 hsa-mir-101 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097673 | Abbexa |
|
|
 hsa-mir-103 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097675 | Abbexa |
|
|
 hsa-mir-105 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097676 | Abbexa |
|
|
 hsa-mir-106a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097677 | Abbexa |
|
|
 hsa-mir-106b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097678 | Abbexa |
|
|
 hsa-mir-107 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097679 | Abbexa |
|
|
 hsa-mir-124 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097680 | Abbexa |
|
|
 hsa-mir-125a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097681 | Abbexa |
|
|
 hsa-mir-127 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097686 | Abbexa |
|
|
 hsa-mir-129 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097689 | Abbexa |
|
|
 hsa-mir-130a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097690 | Abbexa |
|
|
 hsa-mir-130b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097691 | Abbexa |
|
|
 hsa-mir-132 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097692 | Abbexa |
|
|
 hsa-mir-133a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097693 | Abbexa |
|
|
 hsa-mir-133b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097694 | Abbexa |
|
|
 hsa-mir-134 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097695 | Abbexa |
|
|
 hsa-mir-135b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097696 | Abbexa |
|
|
 hsa-mir-137 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097699 | Abbexa |
|
|
 hsa-mir-138 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097700 | Abbexa |
|
|
 hsa-mir-139 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097701 | Abbexa |
|
|
 hsa-mir-140 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097702 | Abbexa |
|
|
 hsa-mir-141 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097703 | Abbexa |
|
|
 hsa-mir-143 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097706 | Abbexa |
|
|
 hsa-mir-145 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097708 | Abbexa |
|
|
 hsa-mir-146a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097709 | Abbexa |
|
|
 hsa-mir-149 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097711 | Abbexa |
|
|
 hsa-mir-150 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097712 | Abbexa |
|
|
 hsa-mir-151 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit |
|||
| 20-abx097714 | Abbexa |
|
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