Transformation of Archaea by Direct PCR Merchandise with its Utility To Directed Evolution

Excessive-efficiency transformation of archaea by direct PCR merchandise with its utility to directed evolution of a thermostable enzyme

Hyperthermophilic archaea with distinctive biochemical and physiological traits are necessary organisms for basic analysis of life science and have nice potential for biotechnological functions. Nonetheless, low transformation effectivity of international DNA molecules impedes developments in genetic modification instruments and industrial functions.

 

  • On this research, we utilized extended overlap extension PCR (POE-PCR) to generate multimeric DNA molecules after which remodeled them into two hyperthermophilic archaea, Thermococcus kodakarensis KOD1 and Pyrococcus yayanosii A1.
  • This research was the primary instance to display the improved transformation efficiencies of POE-PCR merchandise by an element of roughly 100 for T. kodakarensis KOD1 and eight for P. yayanosii A1, respectively, relative to round shuttle plasmids.
  • Moreover, directed evolution of a modestly thermophilic enzyme, Methanothermococcus okinawensis 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), was carried out to acquire extra steady ones on account of excessive transformation effectivity of T. kodakarensis (i.e. ~3 × 104 CFU per μg DNA). T. kodakarensis harbouring essentially the most thermostable MoHMGR mutant can develop within the presence of a thermostable antibiotic simvastatin at 85°C and even greater temperatures.
  • This excessive transformation effectivity method couldn’t solely assist develop extra hyperthermophilic enzyme mutants by way of directed evolution but in addition simplify genetical modification of archaea, which could possibly be novel hosts for industrial biotechnology

Screening of business meat merchandise from grocery store chains for feline derivatives utilizing SP-PCR-RLFP and lab-on-a-chip

Willpower of feline meat in meals merchandise is a vital situation for social, well being, financial and spiritual concern. Therefore this paper documented the applying of species particular polymerase chain reaction-restriction fragment size polymorphism (SP-PCR-RFLP) assay concentrating on a short-fragments (69 bp) of mitochondrial cytochrome b (cytb) gene to display feline meat in industrial meat merchandise utilizing lab-on-a-chip.
The SP-PCR assay proved its specificity theoretically and experimentally whereas testing with totally different widespread animal, aquatic and plant species of DNA. The feline particular (69 bp, 43- and 26-bp) attribute molecular DNA sample was noticed by SP-PCR and RFLP evaluation.

For assay efficiency, it was examined in three several types of industrial dummy meat merchandise equivalent to frankfurters, nuggets and meatballs and digested with AluI-restriction enzyme. The best sensitivity of the assay utilizing lab-on-a-chip was as little as 0.1 pg or 0.01 % (w/w) in industrial dummy meat merchandise. Now we have additionally utilized this assay to display three necessary industrial meat merchandise of six totally different model from six grocery store chains positioned at three totally different states of Malaysia.

Thus whole 378 samples had been examined to validate the specificity, sensitivity, stability of the assay and utilization of it for industrial meat product screening.
Key phrases: Industrial meat merchandise; Feline meat; Halal; Lab-on-a-chip; Restriction digestion.
genpcr

genpcr

XFD594 Phalloidin *XFD594 Same Structure to Alexa Fluor™ 594*

23158 300 Tests
EUR 334

Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated

Ab8011-001 1mg
EUR 400.8

SAM FCM (Alexa Fluor 647)

abx098902-100tests 100 tests
EUR 1479.6

SAM FCM (Alexa Fluor 488)

abx098904-60tests 60 tests
EUR 1629.6

Endoglin/CD105 Alexa Fluor

FC15024 100 Tests
EUR 537.6

XFD594 tyramide reagent *Same Structure to Alexa Fluor™ 594 tyramide*

11082 200 slides
EUR 222

EGFR antibody (Alexa Fluor 488)

61R-E109BAF 125 ug
EUR 847.2
Description: Mouse monoclonal EGFR antibody (Alexa Fluor 488)

XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester*

1830 1 mg
EUR 222

XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester*

71908 5 mg
EUR 781

XFD594-streptavidin conjugate *XFD594 Same Structure to Alexa Fluor™ 594*

16892 1 mg
EUR 166

XFD594 C5 Maleimide *Same Structure to Alexa Fluor™ 594 C5 Maleimide*

1891 1 mg
EUR 222

Annexin V, XFD594 conjugate *XFD594 Same Structure to Alexa Fluor™ 594*

20096 100 tests
EUR 222

Anti-RPSA Alexa Fluor® 488

A4-829-C100 0.1 mg
EUR 372

Concanavalin A, XFD594 Labeled *XFD594 Same Structure to Alexa Fluor™ 594*

25575 1 mg
EUR 102

Wheat Germ Agglutinin, XFD594 Labeled *XFD594 Same Structure to Alexa Fluor™ 594*

25509 1 mg
EUR 102

Goat anti Mouse IgG1 (Alexa Fluor 488)

43R-1649 500 ug
EUR 684
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488)

XFD594 Anti-human CD3 Antibody *SK7, XFD594 Same Structure to Alexa Fluor™ 594*

10033170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD3 Antibody *SK7, XFD594 Same Structure to Alexa Fluor™ 594*

10033171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD4 Antibody *SK3, XFD594 Same Structure to Alexa Fluor™ 594*

10042170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD4 Antibody *SK3, XFD594 Same Structure to Alexa Fluor™ 594*

10042171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD8 Antibody *SK1, XFD594 Same Structure to Alexa Fluor™ 594*

10081170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD8 Antibody *SK1, XFD594 Same Structure to Alexa Fluor™ 594*

10081171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD28 Antibody *9.3, XFD594 Same Structure to Alexa Fluor™ 594*

10281170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD28 Antibody *9.3, XFD594 Same Structure to Alexa Fluor™ 594*

10281171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD64 Antibody *10.1, XFD594 Same Structure to Alexa Fluor™ 594*

10640170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD64 Antibody *10.1, XFD594 Same Structure to Alexa Fluor™ 594*

10640171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD4 Antibody *SK3, XFD594 Same Structure to Alexa Fluor™ 594*

10042170-100tests 100 tests
EUR 245
Description: SK3 is an anti-human monoclonal antibody that recognizes the CD4 antigen.

XFD594 Anti-human CD4 Antibody *SK3, XFD594 Same Structure to Alexa Fluor™ 594*

10042171-500tests 500 tests
EUR 918
Description: SK3 is an anti-human monoclonal antibody that recognizes the CD4 antigen.

XFD594 Anti-human CD8 Antibody *SK1, XFD594 Same Structure to Alexa Fluor™ 594*

10081170-100tests 100 tests
EUR 245
Description: SK1 is an anti-human monoclonal antibody that is specific for the CD8a antigen.

XFD594 Anti-human CD8 Antibody *SK1, XFD594 Same Structure to Alexa Fluor™ 594*

10081171-500tests 500 tests
EUR 918
Description: SK1 is an anti-human monoclonal antibody that is specific for the CD8a antigen.

XFD594 Anti-human CD3 Antibody *SK7, XFD594 Same Structure to Alexa Fluor™ 594*

10033170-100tests 100 tests
EUR 245
Description: The SK7 monoclonal antibody reacts with human CD3e, a 20 kD member of the Ig superfamily often found on the surface of t cells, tregs and nkt cells.

XFD594 Anti-human CD3 Antibody *SK7, XFD594 Same Structure to Alexa Fluor™ 594*

10033171-500tests 500 tests
EUR 918
Description: The SK7 monoclonal antibody reacts with human CD3e, a 20 kD member of the Ig superfamily often found on the surface of t cells, tregs and nkt cells.

XFD594 Anti-human CD64 Antibody *10.1, XFD594 Same Structure to Alexa Fluor™ 594*

10640170-100tests 100 tests
EUR 245
Description: The 10.1 monoclonal antibody binds with human CD64, a 72 kD single-pass type i membrane protein typically expressed on the surface of granulocytes, monocytes and dendritic cells.

XFD594 Anti-human CD64 Antibody *10.1, XFD594 Same Structure to Alexa Fluor™ 594*

10640171-500tests 500 tests
EUR 918
Description: The 10.1 monoclonal antibody binds with human CD64, a 72 kD single-pass type i membrane protein typically expressed on the surface of granulocytes, monocytes and dendritic cells.

XFD594 Anti-human CD28 Antibody *9.3, XFD594 Same Structure to Alexa Fluor™ 594*

10281170-100tests 100 tests
EUR 245
Description: The 9.3 monoclonal antibody binds with human CD28, a 44 kD transmembrane glycoprotein typically located on the surface of natural killer cells and plasma cells.

XFD594 Anti-human CD28 Antibody *9.3, XFD594 Same Structure to Alexa Fluor™ 594*

10281171-500tests 500 tests
EUR 918
Description: The 9.3 monoclonal antibody binds with human CD28, a 44 kD transmembrane glycoprotein typically located on the surface of natural killer cells and plasma cells.

XFD594 Anti-human CD11c Antibody *3.9, XFD594 Same Structure to Alexa Fluor™ 594*

10113170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD11c Antibody *3.9, XFD594 Same Structure to Alexa Fluor™ 594*

10113171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD1 Antibody *SN13, XFD594 Same Structure to Alexa Fluor™ 594*

10012160 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD1 Antibody *SN13, XFD594 Same Structure to Alexa Fluor™ 594*

10012161 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD1 Antibody *L161, XFD594 Same Structure to Alexa Fluor™ 594*

10013160 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD1 Antibody *L161, XFD594 Same Structure to Alexa Fluor™ 594*

10013161 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD9 Antibody *HI9a, XFD594 Same Structure to Alexa Fluor™ 594*

10090170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD9 Antibody *HI9a, XFD594 Same Structure to Alexa Fluor™ 594*

10090171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD7 Antibody *HIT7, XFD594 Same Structure to Alexa Fluor™ 594*

10070170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD7 Antibody *HIT7, XFD594 Same Structure to Alexa Fluor™ 594*

10070171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD16 Antibody *3G8, XFD594 Same Structure to Alexa Fluor™ 594*

10161170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD16 Antibody *3G8, XFD594 Same Structure to Alexa Fluor™ 594*

10161171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD19 Antibody *4G7, XFD594 Same Structure to Alexa Fluor™ 594*

10193170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD19 Antibody *4G7, XFD594 Same Structure to Alexa Fluor™ 594*

10193171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-mouse CD19 Antibody *1D3, XFD594 Same Structure to Alexa Fluor™ 594*

10194160 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-mouse CD19 Antibody *1D3, XFD594 Same Structure to Alexa Fluor™ 594*

10194161 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD38 Antibody *HB7, XFD594 Same Structure to Alexa Fluor™ 594*

10382160 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD38 Antibody *HB7, XFD594 Same Structure to Alexa Fluor™ 594*

10382161 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD40 Antibody *G28.5, XFD594 Same Structure to Alexa Fluor™ 594*

10401171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD32 Antibody *3D3, XFD594 Same Structure to Alexa Fluor™ 594*

10321170 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD32 Antibody *3D3, XFD594 Same Structure to Alexa Fluor™ 594*

10321171 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD45 Antibody *2D1, XFD594 Same Structure to Alexa Fluor™ 594*

10454170 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD45 Antibody *2D1, XFD594 Same Structure to Alexa Fluor™ 594*

10454171 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD72 Antibody *3F3, XFD594 Same Structure to Alexa Fluor™ 594*

10720170 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD72 Antibody *3F3, XFD594 Same Structure to Alexa Fluor™ 594*

10720171 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD73 Antibody *AD2, XFD594 Same Structure to Alexa Fluor™ 594*

10730170 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD73 Antibody *AD2, XFD594 Same Structure to Alexa Fluor™ 594*

10730171 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD82 Antibody *C33, XFD594 Same Structure to Alexa Fluor™ 594*

10820170 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD82 Antibody *C33, XFD594 Same Structure to Alexa Fluor™ 594*

10820171 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD111 Antibody *R1.302, XFD594 Same Structure to Alexa Fluor™ 594*

11110160 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD111 Antibody *R1.302, XFD594 Same Structure to Alexa Fluor™ 594*

11110161 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD112 Antibody *R2.525, XFD594 Same Structure to Alexa Fluor™ 594*

11120160 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD112 Antibody *R2.525, XFD594 Same Structure to Alexa Fluor™ 594*

11120161 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

XFD594 Anti-human CD7 Antibody *HIT7, XFD594 Same Structure to Alexa Fluor™ 594*

10070170-100tests 100 tests
EUR 245
Description: HIT7 is an anti-human monoclonal antibody that recognizes the CD7 antigen.

XFD594 Anti-human CD7 Antibody *HIT7, XFD594 Same Structure to Alexa Fluor™ 594*

10070171-500tests 500 tests
EUR 918
Description: HIT7 is an anti-human monoclonal antibody that recognizes the CD7 antigen.

XFD594 Anti-human CD9 Antibody *HI9a, XFD594 Same Structure to Alexa Fluor™ 594*

10090170-100tests 100 tests
EUR 245
Description: HI9a is an anti-human monoclonal antibody that recognizes the CD9 antigen.

XFD594 Anti-human CD9 Antibody *HI9a, XFD594 Same Structure to Alexa Fluor™ 594*

10090171-500tests 500 tests
EUR 918
Description: HI9a is an anti-human monoclonal antibody that recognizes the CD9 antigen.

XFD594 Anti-human CD11c Antibody *3.9, XFD594 Same Structure to Alexa Fluor™ 594*

10113170-100tests 100 tests
EUR 245
Description: 3.9 is an anti-human monoclonal antibody that forms an immune complex with the CD11c antigen.

XFD594 Anti-human CD11c Antibody *3.9, XFD594 Same Structure to Alexa Fluor™ 594*

10113171-500tests 500 tests
EUR 918
Description: 3.9 is an anti-human monoclonal antibody that forms an immune complex with the CD11c antigen.

XFD594 Anti-human CD16 Antibody *3G8, XFD594 Same Structure to Alexa Fluor™ 594*

10161170-100tests 100 tests
EUR 245
Description: The 3G8 monoclonal antibody recognizes human CD16a, a 50 - 65 kD transmembrane protein typically found on the surface of macrophages, natural killer cells, dendritic cells, monocytes and neutrophils.

XFD594 Anti-human CD16 Antibody *3G8, XFD594 Same Structure to Alexa Fluor™ 594*

10161171-500tests 500 tests
EUR 918
Description: The 3G8 monoclonal antibody recognizes human CD16a, a 50 - 65 kD transmembrane protein typically found on the surface of macrophages, natural killer cells, dendritic cells, monocytes and neutrophils.

Multiplex PCR for simultaneous identification of the most typical European Opisthorchiid and Heterophyid in fish or fish merchandise

Amongst others, the households Opisthorchiidae and Heterophyidae contains a number of genera inflicting fish-borne zoonoses and distributed additionally in European Nations and which can be included within the ParaFishControl (Superior Instruments and Analysis Methods for Parasite Management in European farmed fish) H2020 EU venture.
Because of the small dimension of the metacercariae, the infective stage for human, these parasites can’t be detected visually in fish and monitoring requires professional utility of time-consuming methods. The goal of this was to develop a fast and inexpensive molecular technique primarily based on multiplex PCR for simultaneous identification of metacercariae of the most typical European Opisthorchiid and Heterophyid in fish or fish merchandise.
Key phrases: Europe; Fish merchandise; Freshwater fish; Heterophyidae; Multiplex PCR; Opisthorchiidae
TaqMan probe primarily based multiplex quantitative PCR assay for dedication of bovine, porcine and fish DNA in gelatin admixture, meals merchandise and dietary dietary supplements.
Detection of animal supplies in gelatin-based merchandise is required to deal with spiritual and cultural considerations, as a result of porcine and bovine gelatins are prohibited in Halal, Kosher and Hindus shopper goods. On this paper, multiplex quantitative polymerase chain response (qPCR) assay utilizing TaqMan probe was developed to discriminate bovine, porcine and fish gelatin species in a single assay platform.
The assay was particular to cattle, pigs and fish, having been examined in opposition to 14 non-target species. The restrict of detection, underneath gelatin admixed circumstances, was 0.005 ng/µL. Lastly, a pilot survey was undertaken testing 35 Halal branded processed meals and dietary gadgets. Out of 35 samples, solely two had been discovered to be optimistic for porcine species. The authenticity of those two qPCR merchandise was confirmed by DNA sequencing evaluation, which confirmed 99-100% similarity with Sus scrofa (Wild boar) species.

Eztime Real - time PCRPremix 2x,Taqman

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Eco 48 Real Time PCR system including software and loading dock

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Eztime Real - time PCRPremix 2x,SYBR Green

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hsa-mir-521 Real-time RT-PCR Detection Kit

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Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye

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Novel Coronavirus COVID-19 (2019-nCoV) Real Time Multiplex RT-PCR Kit (Detection for 3 Genes )

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Description: Novel Coronavirus (2019-nCoV) Real Time Multiplex RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems. It detects N gene, E gene and RdRp gene of 2019-nCoV. RR-0479-02 has been also approverd by CFDA for emergency use and is WHO standard.

TurboQ Real-time PCR Machine with Laptop

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dsGreen for Real-Time PCR, 100×, 1 mL

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dsGreen for Real-Time PCR, 100×, 10 mL

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dsGreen for Real-Time PCR, 100×, 5 mL

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dsGreen for Real-Time PCR, 100×, 2 mL

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Azura PureView 1 Kb DNA Ladder - 100 Lanes

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Azura PureView 1 Kb DNA Ladder - 500 Lanes

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Azura PureView 1 Kb DNA Ladder - 1000 Lanes

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Azura PureView 250bp DNA Ladder - 100 Lanes

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Azura PureView 250bp DNA Ladder - 500 Lanes

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Azura PureView 250bp DNA Ladder - 1000 Lanes

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Azura PureView 100bp DNA Ladder - 100 Lanes

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Azura PureView 100bp DNA Ladder - 500 Lanes

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Azura PureView 100bp DNA Ladder - 1000 Lanes

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Azura PureView 50bp DNA Ladder - 100 Lanes

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Azura PureView 50bp DNA Ladder - 500 Lanes

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Azura PureView 50bp DNA Ladder - 1000 Lanes

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AzuraView GreenFast qPCR Blue Mix Lo Rox - 200 Rxn

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AzuraView GreenFast qPCR Blue Mix Lo Rox - 500 Rxn

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AzuraView GreenFast qPCR Blue Mix Lo Rox - 5000 Rxn

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AzuraView GreenFast qPCR Blue Mix Hi Rox - 200 Rxn

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AzuraView GreenFast qPCR Blue Mix Hi Rox - 500 Rxn

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AzuraView GreenFast qPCR Blue Mix Hi Rox - 2000 Rxn

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AzuraView GreenFast qPCR Blue Mix Hi Rox - 5000 Rxn

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Mouse Glucose Metabolism Primer Library

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Mouse Hippo Signaling Primer Library

MHPO-I 1 set
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Mouse Hypoxia Signaling Primer Library

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Mouse Notch Signaling Primer Library

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Mouse Neurotransmitter Signaling Primer Library

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Mouse Integrin Signaling Primer Library

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Mouse Oxidative Stress Primer Library

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Mouse Oncogene Primer Library

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Mouse Apoptosis Primer Library

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Mouse Ras Signaling Primer Library

MRAS-I 1 set
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Mouse T-Cell Receptor Signaling Primer Library

MTCR-I 1 set
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Mouse TGF Beta Signaling Primer Library

MTGFB-I 1 set
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Mouse Tumor Invasion/Metastasis Primer Library

MTIM-I 1 set
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Mouse Tumor Immunity Primer Library

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Mouse p53 Signaling Primer Library

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Mouse WNT Signaling Primer Library

MWNT-I 1 set
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Mouse Toll-like Receptor Signaling Primer Library

newitem 1 set
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Mouse PI3K-AKT Signaling Primer Library

MAKT-I 1 set
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Mouse Angiogenesis Primer Library

MAPL-I 1 set
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Mouse Adherin - Tight Junction Primer Library

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Mouse Autophagy Primer Library

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Mouse Bdnf Signaling Primer Library

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Mouse BMP Signaling Primer Library

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Mouse Cytokine Primer Library I

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Mouse Cytokine Primer Library II

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Mouse Cell Cycle Primer Library

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Mouse Cytokine and Chemokine Receptor Primer Library

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Mouse CREB Signaling Primer Library

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Mouse DNA Damage Signaling Primer Library

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Mouse DNA Repair Primer Library I

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Mouse DNA Repair Primer Library II

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Mouse EGFR Signaling Primer Library

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Mouse Estrogen Receptor Signaling Library

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Mouse Fatty Liver Signaling Primer Library

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Mouse Jak/Stat Signaling Primer Library

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Mouse MAP Kinase Signaling Primer Library

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Mouse NFKappaB Primer Library

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Human DNA Repair Primer Library I

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Human DNA Repair Primer Library II

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Human Drug Detoxication I

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Human Drug Detoxication II

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Human EGFR Signaling Primer Library

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Human Epithelial Mesenchymal Transition Primer Library

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Human Endothelial Cell Primer Library

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Human ERBB Signaling Primer Library

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Human Estrogen Signaling Primer Library

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