Transformation of Archaea by Direct PCR Merchandise with its Utility To Directed Evolution

Posted on by Nicholas under Antibodies, Assay Kits, Biology Cells, cDNA, Clia Kits, Culture Cells, Devices, DNA, DNA Templates, Elisa Kits, Enzymes, Equipments, Exosomes, Gels, Isotypes, Medium & Serums, NATtrol, Panel, Particles, PCR, Pcr Kits, Peptides, Reagents, Recombinant Proteins, Ria Kits, RNA, Test Kits, Vector & Virus, Western Blot

Excessive-efficiency transformation of archaea by direct PCR merchandise with its utility to directed evolution of a thermostable enzyme

Hyperthermophilic archaea with distinctive biochemical and physiological traits are necessary organisms for basic analysis of life science and have nice potential for biotechnological functions. Nonetheless, low transformation effectivity of international DNA molecules impedes developments in genetic modification instruments and industrial functions.

 

  • On this research, we utilized extended overlap extension PCR (POE-PCR) to generate multimeric DNA molecules after which remodeled them into two hyperthermophilic archaea, Thermococcus kodakarensis KOD1 and Pyrococcus yayanosii A1.
  • This research was the primary instance to display the improved transformation efficiencies of POE-PCR merchandise by an element of roughly 100 for T. kodakarensis KOD1 and eight for P. yayanosii A1, respectively, relative to round shuttle plasmids.
  • Moreover, directed evolution of a modestly thermophilic enzyme, Methanothermococcus okinawensis 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), was carried out to acquire extra steady ones on account of excessive transformation effectivity of T. kodakarensis (i.e. ~3 × 104 CFU per μg DNA). T. kodakarensis harbouring essentially the most thermostable MoHMGR mutant can develop within the presence of a thermostable antibiotic simvastatin at 85°C and even greater temperatures.
  • This excessive transformation effectivity method couldn’t solely assist develop extra hyperthermophilic enzyme mutants by way of directed evolution but in addition simplify genetical modification of archaea, which could possibly be novel hosts for industrial biotechnology

Screening of business meat merchandise from grocery store chains for feline derivatives utilizing SP-PCR-RLFP and lab-on-a-chip

Willpower of feline meat in meals merchandise is a vital situation for social, well being, financial and spiritual concern. Therefore this paper documented the applying of species particular polymerase chain reaction-restriction fragment size polymorphism (SP-PCR-RFLP) assay concentrating on a short-fragments (69 bp) of mitochondrial cytochrome b (cytb) gene to display feline meat in industrial meat merchandise utilizing lab-on-a-chip.
The SP-PCR assay proved its specificity theoretically and experimentally whereas testing with totally different widespread animal, aquatic and plant species of DNA. The feline particular (69 bp, 43- and 26-bp) attribute molecular DNA sample was noticed by SP-PCR and RFLP evaluation.

For assay efficiency, it was examined in three several types of industrial dummy meat merchandise equivalent to frankfurters, nuggets and meatballs and digested with AluI-restriction enzyme. The best sensitivity of the assay utilizing lab-on-a-chip was as little as 0.1 pg or 0.01 % (w/w) in industrial dummy meat merchandise. Now we have additionally utilized this assay to display three necessary industrial meat merchandise of six totally different model from six grocery store chains positioned at three totally different states of Malaysia.

Thus whole 378 samples had been examined to validate the specificity, sensitivity, stability of the assay and utilization of it for industrial meat product screening.
Key phrases: Industrial meat merchandise; Feline meat; Halal; Lab-on-a-chip; Restriction digestion.
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Alexa Fluor® 594 anti-mouse CD31
E16FMJ031-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-mouse CD45
E16FMJ045-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-mouse CD68
E16FMJ068-100U 100 μg
EUR 1365
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-human CD8a
E16FHJ008a-100U 100 μg
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-human CD14
E16FHJ0142-100U 100 μg
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-human CD19
E16FHJ019-100U 100 μg
EUR 1083.33
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-mouse CD11c
E16FMJ011c-100U 100 μg
EUR 758.33
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-mouse CD106
E16FMJ106-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-human CD11a
E16FHJ011a-100U 100 μg
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 594 anti-human CD11b
E16FHJ011b-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Mouse Anti-Human IgA - Alexa Fluor 594
DL87387A-100ul 100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgA - Alexa Fluor 594
DL87387A-500ul 500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 594
DL87390A-100ul 100 ul
EUR 155
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 594
DL87390A-500ul 500 ul
EUR 350
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgA - Alexa Fluor 594
E38A3634 100μL
EUR 125

Mouse Anti-Human IgM - Alexa Fluor 594
E38A3638 100μL
EUR 125

Mouse Anti-Human IgA - Alexa Fluor 594
YLF0564-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgA - Alexa Fluor 594
YLF0564-1ml 1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgA - Alexa Fluor 594
YLF0564-500ul 500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 594
YLF0361-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 594
YLF0361-1ml 1 ml
EUR 480
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgM - Alexa Fluor 594
YLF0361-500ul 500 ul
EUR 290
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 594
DL87379A-100ul 100 ul
EUR 190
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 594
DL87379A-500ul 500 ul
EUR 280
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 594
E38A3625 100μL
EUR 125

Rabbit Anti-Human IgM - Alexa Fluor 594
YLF0561-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 594
YLF0561-1ml 1 ml
EUR 300
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgM - Alexa Fluor 594
YLF0561-500ul 500 ul
EUR 210
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594
DL87358A-100ul 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594
DL87358A-500ul 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594
YLF0339-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594
YLF0339-1ml 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fc - Alexa Fluor 594
YLF0339-500ul 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 594
DL87357A-100ul 100 ul
EUR 190
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 594
DL87357A-500ul 500 ul
EUR 280
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 594
DL87389A-100ul 100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 594
DL87389A-500ul 500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Alexa Fluor® 594 anti-mouse CD206 (MMR)
E16FMJ206-100U 100 μg
EUR 1191.67
Description: Available in various conjugation types.

Goat Anti-Human IgG Fab - Alexa Fluor 594
E38A3603 100μL
EUR 125

Mouse Anti-Human IgG Fc - Alexa Fluor 594
E38A3636 100μL
EUR 125

Goat Anti-Human IgG Fab - Alexa Fluor 594
YLF0558-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 594
YLF0558-1ml 1 ml
EUR 300
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG Fab - Alexa Fluor 594
YLF0558-500ul 500 ul
EUR 210
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 594
YLF0566-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 594
YLF0566-1ml 1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fc - Alexa Fluor 594
YLF0566-500ul 500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594
DL87376A-100ul 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594
DL87376A-500ul 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fab - Alexa Fluor 594
DL87388A-100ul 100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fab - Alexa Fluor 594
DL87388A-500ul 500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Alexa Fluor® 594 anti-human/mouse CD44
E16FMJ044-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594
E38A3622 100μL
EUR 125

Mouse Anti-Human IgG Fab - Alexa Fluor 594
E38A3635 100μL
EUR 125

Mouse Anti-Human IgG Fab - Alexa Fluor 594
YLF0565-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fab - Alexa Fluor 594
YLF0565-1ml 1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Human IgG Fab - Alexa Fluor 594
YLF0565-500ul 500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594
YLF0354-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594
YLF0354-1ml 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Rabbit Anti-Human IgG Fc - Alexa Fluor 594
YLF0354-500ul 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Alexa Fluor® 594 anti-human/mouse CD11b
E16FMJ011b-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)
43R-ID022AF 500 ug
EUR 334
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)
43R-ID047AF 500 ug
EUR 465
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594
DL87364A-100ul 100 ul
EUR 155
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594
DL87364A-500ul 500 ul
EUR 350
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594
E38A3610 100μL
EUR 125

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594
YLF0343-100ul 100 ul
EUR 130
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594
YLF0343-1ml 1 ml
EUR 480
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Pig IgG (H&L) - Alexa Fluor 594
YLF0343-500ul 500 ul
EUR 290
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Donkey anti Goat IgG (H + L) (Alexa Fluor 594)
43R-ID005AF 500 ug
EUR 333
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594)

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594
DL87393A-100ul 100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594
DL87393A-500ul 500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594
E38A3641 100μL
EUR 125

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594
YLF0569-100ul 100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594
YLF0569-1ml 1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Mouse Anti-Pig IgG (H&L) - Alexa Fluor 594
YLF0569-500ul 500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG (H&L) - Alexa Fluor 594
DL87356A-100ul 100 ul
EUR 140
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Goat Anti-Human IgG (H&L) - Alexa Fluor 594
DL87356A-500ul 500 ul
EUR 330
Description: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Multiplex PCR for simultaneous identification of the most typical European Opisthorchiid and Heterophyid in fish or fish merchandise

Amongst others, the households Opisthorchiidae and Heterophyidae contains a number of genera inflicting fish-borne zoonoses and distributed additionally in European Nations and which can be included within the ParaFishControl (Superior Instruments and Analysis Methods for Parasite Management in European farmed fish) H2020 EU venture.
Because of the small dimension of the metacercariae, the infective stage for human, these parasites can’t be detected visually in fish and monitoring requires professional utility of time-consuming methods. The goal of this was to develop a fast and inexpensive molecular technique primarily based on multiplex PCR for simultaneous identification of metacercariae of the most typical European Opisthorchiid and Heterophyid in fish or fish merchandise.
Key phrases: Europe; Fish merchandise; Freshwater fish; Heterophyidae; Multiplex PCR; Opisthorchiidae
TaqMan probe primarily based multiplex quantitative PCR assay for dedication of bovine, porcine and fish DNA in gelatin admixture, meals merchandise and dietary dietary supplements.
Detection of animal supplies in gelatin-based merchandise is required to deal with spiritual and cultural considerations, as a result of porcine and bovine gelatins are prohibited in Halal, Kosher and Hindus shopper goods. On this paper, multiplex quantitative polymerase chain response (qPCR) assay utilizing TaqMan probe was developed to discriminate bovine, porcine and fish gelatin species in a single assay platform.
The assay was particular to cattle, pigs and fish, having been examined in opposition to 14 non-target species. The restrict of detection, underneath gelatin admixed circumstances, was 0.005 ng/µL. Lastly, a pilot survey was undertaken testing 35 Halal branded processed meals and dietary gadgets. Out of 35 samples, solely two had been discovered to be optimistic for porcine species. The authenticity of those two qPCR merchandise was confirmed by DNA sequencing evaluation, which confirmed 99-100% similarity with Sus scrofa (Wild boar) species.

HDV Real Time RT-PCR Kit
GWB-LRB030 25 tests Ask for price

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ONE STEP RT-PCR REAL TIME KIT
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EUR 314.51
Description: ONE STEP RT-PCR REAL TIME KIT

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HCV Genetype Real Time RT-PCR Kit
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RV Real Time RT-PCR Detection kit
TRI-L02M1 32T
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RV Real Time RT-PCR Detection kit
TRI-L02S1 16T
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Measles Virus Real Time RT-PCR Kit
GWB-LRB043 25 tests Ask for price

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Rubella Virus Real Time RT-PCR Kit
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FCV Real Time RT-PCR Detection kit
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FCV Real Time RT-PCR Detection kit
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TRI-L22M1 32T
EUR 705.6

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TRI-L22S1 16T
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HLA B27 Real-TM Real Time PCR kit
R116-50FRT 48
EUR 627.84

FIPV Real Time RT-PCR Detection kit
TRI-L03M1 32T
EUR 453.6

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TRI-L03S1 16T
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TRI-L28M1 48T
EUR 1008

CSFV Real Time RT-PCR Detection kit
TRI-L28S1 24T
EUR 529.2

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TRI-L34M1 48T
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CP&MP Real Time PCR Kit
GWB-LRB005 25 tests Ask for price

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MRSA Quant Real-TM Real Time PCR kit
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Chikungunya Virus Real Time RT-PCR Kit
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Yersinia pestis Real-TM Real Time PCR kit
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Monkeypox Virus Real Time PCR Kit
PDPS-AR064 1 unit Ask for price
Description: Creative Biogene Monkeypox Virus Real Time PCR Kit is used for the detection of monkeypox Virus in serum or lesion exudate samples by using real time PCR systems. Monkeypox virus (MPV) is a double-stranded DNA, zoonotic virus and a species of the genus Orthopoxvirus in the family Poxviridae. It is one of the human orthopoxviruses that includes variola (VARV), cowpox (CPX), and vaccinia (VACV) viruses. The kit contains a specific ready-to-use system for the detection of the monkeypox Virus. Fluorescence is emitted and measured by the real time systems' optical unit during the PCR.

VetAlert Johnes Real Time PCR Kit
TC-9828-100 100 rxns
EUR 460

Monkeypox Virus Real Time PCR Kit
ZD-0076-01 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit
ZD-0076-02 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit
YJC70115NW-25T 25 tests/kit Ask for price
Description: The Bioperfectus Monkeypox Virus Real Time PCR Kit is an in vitro diagnostic test, based on real-time PCR technology, for the detection of DNA from the Monkeypox virus. Specimens can be obtained from human serum, lesion exudate samples and scab. BSL-2 facilities with standard BSL-2 work practices may be used for the test of t he Monkeypox virus.

Staphilococcus aureus Real-TM Real Time PCR kit
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HBV Quantitative Real Time PCR Kit
GWB-LRB013 25 tests Ask for price

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Toxoplasma Gondii Real Time PCR Kit
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Human Lung Cancer PCR Primer Library
HLUCPL-I 1 set
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Human Schwann Cell PCR Primer Library
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HEV I & III Real Time RT-PCR Kit
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CHV Real Time PCR Detection kit
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CHV Real Time PCR Detection kit
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PCVⅡ Real Time PCR Detection kit
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PCVⅡ Real Time PCR Detection kit
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HEV General Type Real Time RT-PCR Kit
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BioRT Real Time RT-PCR Kit（SYBR Green）
TRI-B33M1 100T
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BioRT Real Time RT-PCR Kit（SYBR Green）
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ASFV Real Time PCR Detection kit
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ASFV Real Time PCR Detection kit
TRI-L04S1C 24T
EUR 302.4

Babesia Real Time PCR Detection kit
TRI-L20M1 32T
EUR 453.6

Babesia Real Time PCR Detection kit
TRI-L20S1 16T
EUR 252

Giardia Real Time PCR Detection kit
TRI-L26M1 32T
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Giardia Real Time PCR Detection kit
TRI-L26S1 16T
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Oncogenetics BRCA Panel Real-TM Real Time PCR kit
R-27-P-48FRT 48
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Brucella Real Time PCR Detection kit
TRI-L24M1 32T
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Brucella Real Time PCR Detection kit
TRI-L24S1 16T
EUR 252

Chlamydia Real Time PCR Detection kit
TRI-L15M1 32T
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Chlamydia Real Time PCR Detection kit
TRI-L15S1 16T
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Leptospira Real Time PCR Detection kit
TRI-L13M1 32T
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Leptospira Real Time PCR Detection kit
TRI-L13S1 16T
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Mycoplasma Real Time PCR Detection kit
TRI-L16M1 32T
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Ehrilichia Real Time PCR Detection kit
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PRRSV Universal Real Time RT-PCR Detection kit
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PRRSV Universal Real Time RT-PCR Detection kit
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Edwardsiella Real Time PCR Detection kit
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Edwardsiella Real Time PCR Detection kit
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HCV/HBV/HIV1/HIV2 Real-TM Real Time PCR kit
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Chlamydia Pheumoniae (CP) Real Time PCR Kit
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PRV (gE) Real Time PCR Detection kit
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COMT Val158Met SNP-Screen Real-TM Real Time PCR kit
T01352-50-T 60
EUR 538.46

FHV-1 Real Time PCR Detection kit
TRI-L01M1 32T
EUR 453.6

FHV-1 Real Time PCR Detection kit
TRI-L01S1 16T
EUR 252

BP/MP/CP Multiplex Real Time PCR Kit
GWB-LRB007 25 tests Ask for price

BP/MP/CP Multiplex Real Time PCR Kit
GWB-LRB008 25 tests Ask for price

Fcov and Ccov Real Time RT-PCR Detection kit
TRI-L11M1 32T
EUR 705.6

Fcov and Ccov Real Time RT-PCR Detection kit
TRI-L11S1 16T
EUR 378
Nicholas

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Nicholas

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