Gene targeting PCR assay for the detection of Crocodylus porosus

Double gene focusing on PCR assay for the detection of Crocodylus porosus in industrial merchandise.

The demand for crocodile meat is rapidly rising due to its unique and organoleptic attraction and likewise the low content material of ldl cholesterol and lipids. Furthermore, crocodile oil and blood have been utilized in various medicines for treating bronchial asthma and several other different illnesses since historical occasions. Moreover, crocodile hides have nice demand in leather-based industries.

 

  • All of those have collectively contributed to the intensive searching, unlawful buying and selling and consequent decline of crocodiles in most elements of the world. To maintain area with the rising calls for, some crocodile species comparable to Crocodylus porosus have been raised in farms and its industrial trades have been legalised.
  • Nevertheless, demand for wild crocodiles in meals and medicines has continued in excessive gear. Not too long ago, a number of DNA-based strategies have been proposed for crocodile detection, however these assays are primarily based on single gene and longer-sized amplicon targets that break down throughout intensive processing.
  • To handle this hole, right here we developed and validated a extremely secure double gene focused multiplex PCR assay for the identification of C. porosus supplies in industrial merchandise. The assay concerned two quick websites from C. porosus atp6 (77 bp) and cytb (127 bp) genes and a common inside management (99 bp) for eukaryotes.
  • The PCR primers had been cross-tested in opposition to 18 species and validated underneath pure and blended matrices underneath intensive boiling, autoclaving and microwave cooking circumstances.
  • Lastly, it was used to determine 5 crocodile-based industrial merchandise. The decrease limits of detection for atp6 and cytb genes had been 0.001 ng and 0.01 ng DNA, respectively, in pure meat and 1% underneath blended matrices.
  • Some inherent options, comparable to 77-127 bp amplicon sizes, distinctive stability and superior sensitivity, prompt the assay could possibly be used for the identification of C. porosus in any forensic specimen.

A Quick and Dependable Actual-Time PCR Technique for Detection of Ten Animal Species in Meat Merchandise.

Species substitution in meat merchandise is a typical drawback reported worldwide. One of these meals fraud is, usually, an intentional act for financial achieve, utilizing sources of low-priced meats in high-value meat merchandise. Penalties embody financial, well being, and non secular issues. Extremely delicate and environment friendly methods are thus required to detect meat species.

 

This paper describes a way primarily based on real-time PCR to detect 10 animal species (Bos taurus, Sus scrofa, Ovis aries, Capra hircus, Gallus gallus, Meleagris gallopavo, Bubalus bubalis, Equus caballus, Felis catus, and Canis familiaris) in meat product. The strategy combines species-specific and common (used right here as inside constructive management) primers, and applies soften curve evaluation for amplicon checking. Technique accuracy was evaluated on 46 experimental meat mixtures and all species had been accurately recognized in all instances, at 1% take a look at sensitivity.

 

Evaluation of 14 industrial meat merchandise revealed that 6 of 14 samples had nondeclared bovine and/or rooster materials. We carried out an interlaboratory comparability utilizing the reference meat mixtures and industrial samples, attaining 100% of reproducibility. The developed take a look at proved to be efficient and dependable for routine evaluation of meat merchandise.

genpcr

genpcr

Equine Small Intestine Paraffin Sections

EP-306 10 slides
EUR 240

Chicken Small Intestine cDNA

CD-306 30 reactions
EUR 243

cDNA - Lupus: Small Intestine

C1236226Lup 40 reactions
EUR 811

Cat Small Intestine, Ileum cDNA

FD-309 30 Reactions
EUR 280

Dog Small Intestine, Ileum cDNA

DD-309 30 Reactions
EUR 280

Pig Small Intestine, Ileum cDNA

PD-309 30 reactions
EUR 243

Cat Small Intestine, Jejunum cDNA

FD-308 30 Reactions
EUR 280

Dog Small Intestine, Jejunum cDNA

DD-308 30 Reactions
EUR 280

Pig Small Intestine, Jejunum cDNA

PD-308 30 reactions
EUR 243

Sheep Small Intestine, Ileum cDNA

SD-309 30 reactions
EUR 243

Cat Small Intestine, Duodenum cDNA

FD-307 30 Reactions
EUR 280

Dog Small Intestine, Duodenum cDNA

DD-307 30 Reactions
EUR 280

Bovine Small Intestine, Ileum cDNA

BD-309 30 reactions
EUR 243

Rabbit Small Intestine, Ileum cDNA

TD-309 30 reactions
EUR 243

Pig Small Intestine, Duodenum cDNA

PD-307 30 reactions
EUR 243

Hamster Small Intestine, Ileum cDNA

AD-309 30 reactions
EUR 243

MiniPig Small Intestine, Ileum cDNA

ND-309 30 reactions
EUR 358

Sheep Small Intestine, Jejunum cDNA

SD-308 30 reactions
EUR 243

Human Small Intestine cDNA-Oligo-dT

HD-306 30 reactions
EUR 280

Bovine Small Intestine, Jejunum cDNA

BD-308 30 reactions
EUR 243

Rabbit Small Intestine, Jejunum cDNA

TD-308 30 reactions
EUR 243

Sheep Small Intestine, Duodenum cDNA

SD-307 30 reactions
EUR 243

Hamster Small Intestine, Jejunum cDNA

AD-308 30 reactions
EUR 243

Bovine Small Intestine, Duodenum cDNA

BD-307 30 reactions
EUR 243

cDNA - Liver Cirrhosis: Small Intestine

C1236226Lcs 40 reactions
EUR 802

Rabbit Small Intestine, Duodenum cDNA

TD-307 30 reactions
EUR 243

MiniPig Small Intestine, Jejunum cDNA

ND-308 30 reactions
EUR 358

Hamster Small Intestine, Duodenum cDNA

AD-307 30 reactions
EUR 243

MiniPig Small Intestine, Duodenum cDNA

ND-307 30 reactions
EUR 358

Human Small Intestine cDNA-Random Primer

HD-306-HR 30 reactions
EUR 280

Guinea Pig Small Intestine, Ileum cDNA

GD-309 30 reactions
EUR 243

cDNA - Rat Normal Tissue: Small Intestine

C1434226 40 reactions
EUR 636

cDNA - Dog Normal Tissue: Small Intestine

C1734226 40 reactions
EUR 424

Guinea Pig Small Intestine, Jejunum cDNA

GD-308 30 reactions
EUR 243

cDNA - Mouse Normal Tissue: Small Intestine

C1334226 40 reactions
EUR 636

Guinea Pig Small Intestine, Duodenum cDNA

GD-307 30 reactions
EUR 243

Monkey Small Intestine, Ileum cDNA, Rhesus

UD-309 30 reactions
EUR 316

cDNA - Liver Cirrhosis: Small Intestine: Ileum

C1236227Lcs 40 reactions
EUR 802

Monkey Small Intestine, Jejunum cDNA, Rhesus

UD-308 30 reactions
EUR 316

Monkey Small Intestine, Duodenum cDNA, Rhesus

UD-307 30 reactions
EUR 316

Equine Intestine Genomic DNA

GE-306 0.1mg
EUR 210

cDNA - Liver Cirrhosis: Small Intestine: Jejunum

C1236230Lcs 40 reactions
EUR 802

Monkey Small Intestine, Ileum cDNA, Cynomolgus

KD-309 30 reactions
EUR 316

cDNA - Liver Cirrhosis: Small Intestine: Duodenum

C1236101Lcs 40 reactions
EUR 802

Monkey Small Intestine, Jejunum cDNA, Cynomolgus

KD-308 30 reactions
EUR 316

Monkey Small Intestine, Duodenum cDNA, Cynomolgus

KD-307 30 reactions
EUR 316

cDNA - Human Adult Normal Tissue: Small Intestine

C1234226 40 reactions
EUR 424

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine

C1534226 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Small Intestine

C1534226-Cy 40 reactions
EUR 723

cDNA - Human Adult Normal Tissue: Small Intestine: Ileum

C1234227 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Small Intestine: Jejunum

C1234230 40 reactions
EUR 424

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine: Ileum

C1534227 40 reactions
EUR 723

cDNA - Human Adult Normal Tissue: Small Intestine: Duodenum

C1234101-10 10 reactions
EUR 237

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine: Jejunum

C1534230 40 reactions
EUR 723

Monkey Small Intestine, Ileum cDNA-Random Primer, Rhesus

UD-309-RH 30 reactions
EUR 316

cDNA - Human Diabetic Diseased Tissue: Small Intestine: Ileum

C1236227Dia 40 reactions
EUR 802

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine: Duodenum

C1534101 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Small Intestine: Ileum

C1534227-Cy 40 reactions
EUR 723

Monkey Small Intestine, Jejunum cDNA-Random Primer, Rhesus

UD-308-RH 30 reactions
EUR 316

cDNA - Human Diabetic Diseased Tissue: Small Intestine: Jejunum

C1236230Dia 40 reactions
EUR 802

Monkey Small Intestine, Duodenum cDNA-Random Primer, Rhesus

UD-307-RH 30 reactions
EUR 316

cDNA - Human Diabetic Diseased Tissue: Small Intestine: Duodenum

C1236101Dia 40 reactions
EUR 802

cDNA - Monkey (Cynomolgus) Normal Tissue: Small Intestine: Jejunum

C1534230-Cy 40 reactions
EUR 723

Monkey Small Intestine, Ileum cDNA-Random Primer, Cynomolgus

KD-309-RH 30 reactions
EUR 316

cDNA - Monkey (Cynomolgus) Normal Tissue: Small Intestine: Duodenum

C1534101-Cy 40 reactions
EUR 723

Monkey Small Intestine, Jejunum cDNA-Random Primer, Cynomolgus

KD-308-RH 30 reactions
EUR 316

Monkey Small Intestine, Duodenum cDNA-Random Primer, Cynomolgus

KD-307-RH 30 reactions
EUR 316

UNASSIGNED

This paper describes a quick and dependable methodology for species detection in meat merchandise primarily based on real-time PCR. It may be utilized for evaluation of in natura or processed meat. The strategy proposed right here can play an vital position in controlling the origin of meat merchandise, guaranteeing their high quality and security for the complete meals industry-producers to shoppers.

A common mini-vector and an annealing of PCR merchandise (APP)-based cloning technique for handy molecular organic manipulations.

Presently, essentially the most extensively used methods for molecular cloning are sticky-end ligation-based cloning, TA cloning, blunt-end ligation-based cloning and ligase-independent cloning. On this examine we have now developed a novel mini-vector pANY1 which may concurrently meet the necessities of all these cloning methods.

As well as, the choice of applicable restriction digestion websites is tough in some instances due to the presence of inside websites. On this examine, an annealing of PCR merchandise (APP)-based sticky-end cloning technique was launched to keep away from this challenge. Moreover, false positives happen throughout molecular cloning, which will increase the workload of isolating constructive clones.

The plasmid pANY1 incorporates a ccdB cassette between a number of cloning websites, which effectively avoids these false positives. Subsequently, this mini-vector ought to function a great tool with large functions in biosciences, agriculture, meals applied sciences.

 

Dog Small Intestine, Jejunum cDNA

DD-308 30 Reactions
EUR 280

Cat Small Intestine, Jejunum cDNA

FD-308 30 Reactions
EUR 280

Pig Small Intestine, Jejunum cDNA

PD-308 30 reactions
EUR 243

Sheep Small Intestine, Ileum cDNA

SD-309 30 reactions
EUR 243

Dog Small Intestine, Duodenum cDNA

DD-307 30 Reactions
EUR 280

Bovine Small Intestine, Ileum cDNA

BD-309 30 reactions
EUR 243

Cat Small Intestine, Duodenum cDNA

FD-307 30 Reactions
EUR 280

Pig Small Intestine, Duodenum cDNA

PD-307 30 reactions
EUR 243

Rabbit Small Intestine, Ileum cDNA

TD-309 30 reactions
EUR 243

Hamster Small Intestine, Ileum cDNA

AD-309 30 reactions
EUR 243

MiniPig Small Intestine, Ileum cDNA

ND-309 30 reactions
EUR 358

Sheep Small Intestine, Jejunum cDNA

SD-308 30 reactions
EUR 243

cDNA - Human Adult Normal Tissue: Small Intestine

C1234226 40 reactions
EUR 424

Bovine Small Intestine, Jejunum cDNA

BD-308 30 reactions
EUR 243

Sheep Small Intestine, Duodenum cDNA

SD-307 30 reactions
EUR 243

Rabbit Small Intestine, Jejunum cDNA

TD-308 30 reactions
EUR 243

Bovine Small Intestine, Duodenum cDNA

BD-307 30 reactions
EUR 243

cDNA - Liver Cirrhosis: Small Intestine

C1236226Lcs 40 reactions
EUR 802

Hamster Small Intestine, Jejunum cDNA

AD-308 30 reactions
EUR 243

MiniPig Small Intestine, Jejunum cDNA

ND-308 30 reactions
EUR 358

Rabbit Small Intestine, Duodenum cDNA

TD-307 30 reactions
EUR 243

Hamster Small Intestine, Duodenum cDNA

AD-307 30 reactions
EUR 243

MiniPig Small Intestine, Duodenum cDNA

ND-307 30 reactions
EUR 358

Guinea Pig Small Intestine, Ileum cDNA

GD-309 30 reactions
EUR 243

cDNA - Dog Normal Tissue: Small Intestine

C1734226 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Small Intestine

C1434226 40 reactions
EUR 636

Guinea Pig Small Intestine, Jejunum cDNA

GD-308 30 reactions
EUR 243

cDNA - Human Adult Normal Tissue: Small Intestine: Ileum

C1234227 40 reactions
EUR 424

cDNA - Mouse Normal Tissue: Small Intestine

C1334226 40 reactions
EUR 636

Guinea Pig Small Intestine, Duodenum cDNA

GD-307 30 reactions
EUR 243

Monkey Small Intestine, Ileum cDNA, Rhesus

UD-309 30 reactions
EUR 316

cDNA - Human Adult Normal Tissue: Small Intestine: Jejunum

C1234230 40 reactions
EUR 424

cDNA - Liver Cirrhosis: Small Intestine: Ileum

C1236227Lcs 40 reactions
EUR 802

Monkey Small Intestine, Jejunum cDNA, Rhesus

UD-308 30 reactions
EUR 316

cDNA - Human Adult Normal Tissue: Small Intestine: Duodenum

C1234101-10 10 reactions
EUR 237

Monkey Small Intestine, Duodenum cDNA, Rhesus

UD-307 30 reactions
EUR 316

cDNA - Liver Cirrhosis: Small Intestine: Jejunum

C1236230Lcs 40 reactions
EUR 802

Monkey Small Intestine, Ileum cDNA, Cynomolgus

KD-309 30 reactions
EUR 316

cDNA - Human Diabetic Diseased Tissue: Small Intestine: Ileum

C1236227Dia 40 reactions
EUR 802

cDNA - Liver Cirrhosis: Small Intestine: Duodenum

C1236101Lcs 40 reactions
EUR 802

Monkey Small Intestine, Jejunum cDNA, Cynomolgus

KD-308 30 reactions
EUR 316

cDNA - Human Diabetic Diseased Tissue: Small Intestine: Jejunum

C1236230Dia 40 reactions
EUR 802

Monkey Small Intestine, Duodenum cDNA, Cynomolgus

KD-307 30 reactions
EUR 316

cDNA - Human Diabetic Diseased Tissue: Small Intestine: Duodenum

C1236101Dia 40 reactions
EUR 802

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine

C1534226 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Small Intestine

C1534226-Cy 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine: Ileum

C1534227 40 reactions
EUR 723

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine: Jejunum

C1534230 40 reactions
EUR 723

Monkey Small Intestine, Ileum cDNA-Random Primer, Rhesus

UD-309-RH 30 reactions
EUR 316

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine: Duodenum

C1534101 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Small Intestine: Ileum

C1534227-Cy 40 reactions
EUR 723

Monkey Small Intestine, Jejunum cDNA-Random Primer, Rhesus

UD-308-RH 30 reactions
EUR 316

Monkey Small Intestine, Duodenum cDNA-Random Primer, Rhesus

UD-307-RH 30 reactions
EUR 316

Human Small Intestine Total RNA

HR-306 0.05mg
EUR 172

cDNA - Monkey (Cynomolgus) Normal Tissue: Small Intestine: Jejunum

C1534230-Cy 40 reactions
EUR 723

Monkey Small Intestine, Ileum cDNA-Random Primer, Cynomolgus

KD-309-RH 30 reactions
EUR 316

cDNA - Monkey (Cynomolgus) Normal Tissue: Small Intestine: Duodenum

C1534101-Cy 40 reactions
EUR 723

Monkey Small Intestine, Jejunum cDNA-Random Primer, Cynomolgus

KD-308-RH 30 reactions
EUR 316

Human Small Intestine Tumor lysate

HTL-1338 1 mg
EUR 927.6

Monkey Small Intestine, Duodenum cDNA-Random Primer, Cynomolgus

KD-307-RH 30 reactions
EUR 316

Human Small Intestine Total Protein

HT-306 1mg
EUR 176

Human Small Intestine Epithelial Cells

ABC-TC3814 1 vial Ask for price
Description: Human small intestines cells are derived from whole small intestines that have been dissociated into multi-cellular aggregates and single cells then frozen. Human whole small intestines cells are from a single donor. The small intestine consists of several specialized epithelial cells that could help researchers determine the absorption potential of drug candidates. In addition, these cells can be used with studies of transport and metabolism of drugs and dietary chemicals.

Human Small Intestine Epithelial Cells

ALHE42 1ml frozen Vial
EUR 620

Small Intestine Lysate

1308 0.1 mg
EUR 229.2
Description: Small intestine tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Small Intestine Lysate

1468 0.1 mg
EUR 229.2
Description: Small Intestine tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Small intestine cancer

SM208 each
EUR 546
Description: Small intestine cancer, metastatic lymph nodes and normal tissue high density tissue microarray, 69 cases/208 core, with stage and grade data

Human Small Intestine Endothelial Cells

ABC-TC3813 1 vial Ask for price
Description: Human Intestinal Microvascular Endothelial Cells were initiated by elutriation from dissociated normal human small intestine tissue.These cells were originated using Complete Serum-Free Medium Kit With AcceSup™, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5×10^6 cells. This vial will initiate a Passage 4 cell culture in a 75cm2 flask.

Human Small Intestine Endothelial Cells

HEC15 500,000+ Cells Frozen
EUR 1296

Small intestine disease spectrum (small intestine cancer progression) tissue array

SM802 each
EUR 306
Description: Small intestine disease spectrum (small intestine cancer progression) tissue array, with grade, stage and TNM data, 72 cases/80 cores

Human Small Intestine Tumor Tissue Lysate

IHUINTSTLT100UG each
EUR 361
Description: Human Small Intestine Tumor Tissue Lysate

Human Small Intestine, Ileum Frozen Sections

HF-309 10slides
EUR 240

Human Small Intestine, Jejunum Frozen Sections

HF-308 10slides
EUR 240
Nicholas

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