Gene targeting PCR assay for the detection of Crocodylus porosus

Double gene focusing on PCR assay for the detection of Crocodylus porosus in industrial merchandise.

The demand for crocodile meat is rapidly rising due to its unique and organoleptic attraction and likewise the low content material of ldl cholesterol and lipids. Furthermore, crocodile oil and blood have been utilized in various medicines for treating bronchial asthma and several other different illnesses since historical occasions. Moreover, crocodile hides have nice demand in leather-based industries.

 

  • All of those have collectively contributed to the intensive searching, unlawful buying and selling and consequent decline of crocodiles in most elements of the world. To maintain area with the rising calls for, some crocodile species comparable to Crocodylus porosus have been raised in farms and its industrial trades have been legalised.
  • Nevertheless, demand for wild crocodiles in meals and medicines has continued in excessive gear. Not too long ago, a number of DNA-based strategies have been proposed for crocodile detection, however these assays are primarily based on single gene and longer-sized amplicon targets that break down throughout intensive processing.
  • To handle this hole, right here we developed and validated a extremely secure double gene focused multiplex PCR assay for the identification of C. porosus supplies in industrial merchandise. The assay concerned two quick websites from C. porosus atp6 (77 bp) and cytb (127 bp) genes and a common inside management (99 bp) for eukaryotes.
  • The PCR primers had been cross-tested in opposition to 18 species and validated underneath pure and blended matrices underneath intensive boiling, autoclaving and microwave cooking circumstances.
  • Lastly, it was used to determine 5 crocodile-based industrial merchandise. The decrease limits of detection for atp6 and cytb genes had been 0.001 ng and 0.01 ng DNA, respectively, in pure meat and 1% underneath blended matrices.
  • Some inherent options, comparable to 77-127 bp amplicon sizes, distinctive stability and superior sensitivity, prompt the assay could possibly be used for the identification of C. porosus in any forensic specimen.

A Quick and Dependable Actual-Time PCR Technique for Detection of Ten Animal Species in Meat Merchandise.

Species substitution in meat merchandise is a typical drawback reported worldwide. One of these meals fraud is, usually, an intentional act for financial achieve, utilizing sources of low-priced meats in high-value meat merchandise. Penalties embody financial, well being, and non secular issues. Extremely delicate and environment friendly methods are thus required to detect meat species.

 

This paper describes a way primarily based on real-time PCR to detect 10 animal species (Bos taurus, Sus scrofa, Ovis aries, Capra hircus, Gallus gallus, Meleagris gallopavo, Bubalus bubalis, Equus caballus, Felis catus, and Canis familiaris) in meat product. The strategy combines species-specific and common (used right here as inside constructive management) primers, and applies soften curve evaluation for amplicon checking. Technique accuracy was evaluated on 46 experimental meat mixtures and all species had been accurately recognized in all instances, at 1% take a look at sensitivity.

 

Evaluation of 14 industrial meat merchandise revealed that 6 of 14 samples had nondeclared bovine and/or rooster materials. We carried out an interlaboratory comparability utilizing the reference meat mixtures and industrial samples, attaining 100% of reproducibility. The developed take a look at proved to be efficient and dependable for routine evaluation of meat merchandise.

genpcr

genpcr

cDNA from Liver Cirrhosis: Small Intestine: Jejunum

C1236230Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Small Intestine

C1334226 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Small Intestine

C1434226 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Small Intestine

C1734226 40 reactions
EUR 451.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Small Intestine

C1234226 40 reactions
EUR 451.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Diabetic Tissue: Small Intestine: Duodenum

C1236101Dia 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Diabetic Tissue: Small Intestine: Ileum

C1236227Dia 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Diabetic Tissue: Small Intestine: Jejunum

C1236230Dia 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine

C1534226 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine

C1534226-Cy 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Small Intestine: Duodenum

C1234101-10 10 reactions
EUR 277.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Small Intestine: Ileum

C1234227 40 reactions
EUR 451.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Small Intestine: Jejunum

C1234230 40 reactions
EUR 451.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine: Duodenum

C1534101 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine: Duodenum

C1534101-Cy 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine: Ileum

C1534227 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine: Ileum

C1534227-Cy 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine: Jejunum

C1534230 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine: Jejunum

C1534230-Cy 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Small Intestine Lysate

1468 0.1 mg
EUR 229.2
Description: Small Intestine tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Small Intestine Lysate

1308 0.1 mg
EUR 229.2
Description: Small intestine tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Small intestine cancer

SM208 each
EUR 546
Description: Small intestine cancer, metastatic lymph nodes and normal tissue high density tissue microarray, 69 cases/208 core, with stage and grade data

Small intestine disease spectrum (small intestine cancer progression) tissue array

SM802 each
EUR 306
Description: Small intestine disease spectrum (small intestine cancer progression) tissue array, with grade, stage and TNM data, 72 cases/80 cores

Small Intestine Tumor Lysate

1338 0.1 mg
EUR 304.8
Description: Small Intestine tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Small Intestine Cytoplasmic Lysate

XBL-10875 0.1 mg
EUR 273.3
Description: Human small intestine tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human small intestine tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated small intestine tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated small intestine tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

Small Intestine Membrane Lysate

XBL-10876 0.1 mg
EUR 619.8
Description: Human small intestine tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human small intestine tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated small intestine tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated small intestine tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Small intestine Tissue Slide (Normal)

11-801-10um 10 um
EUR 241.8

Small intestine Tissue Slide (Normal)

11-801-4um 4 um
EUR 216.6

Small Intestine Tissue Slide (Malignant)

11-814-10um 10 um
EUR 241.8

Small Intestine Tissue Slide (Malignant)

11-814-4um 4 um
EUR 216.6

Small Intestine Tissue Slide (Adenocarcinoma)

11-815-10um 10 um
EUR 241.8

Small Intestine Tissue Slide (Adenocarcinoma)

11-815-4um 4 um
EUR 216.6

Small Intestine Tissue Slide (Abnormal)

11-824-10um 10 um
EUR 241.8

Small Intestine Tissue Slide (Abnormal)

11-824-4um 4 um
EUR 216.6

Human Small Intestine Endothelial Cells

HEC15 500,000+ Cells Frozen
EUR 1296

Human Small Intestine Tumor lysate

HTL-1338 1 mg
EUR 927.6

Small Intestine Liver Cirrhosis Lysate

XBL-10377 0.1 mg
EUR 796.2
Description: Human small intestine tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human small intestine tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the small intestine tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The small intestine tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Small Intestine Membrane Tumor Lysate

XBL-10888 0.1 mg
EUR 752.1
Description: Human small intestine tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human small intestine tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated small intestine tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated small intestine tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Normal small intestine tissue array

SMN601 each
EUR 222
Description: Normal small intestine tissue array, including TNM, clinical stage and pathology grade, 30 cases/ 60 cores

Mouse Small Intestine Whole tissue lysate

MAL-1408 1 mg
EUR 628.8

Rat Small Intestine Whole tissue lysate

RAL-1468 1 mg
EUR 628.8

Total RNA from Lupus: Small Intestine

R1236226Lup-50 50 ug
EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Small intestine carcinoma test tissue array

T421b each
EUR 48
Description: Small intestine carcinoma test tissue array, with normal small intestine tissues, including pathology grade, TNM and clinical stage, 6 cases/24 cores, replacing T421a

Matched Pair Tissue Slides - Small Intestine Tissue

11-800-MPPT 1 pack
EUR 241.8
Description: The Matched Pair Paraffin Tissue (MPPT) slides are designed for identifying tumor-specific/metastasis genes or proteins. Slices from normal and malignant tissues are mounted on each MPPT slide which can then be treated as a single histological slide for H&E staining, immunohistochemistry, or in situ hybridization. This format allows a rapid analysis of protein expression and localization across normal and cancerous tissue.

Multiple Species Tissue Slides - Small Intestine Tissue

11-800-MSTA 1 pack
EUR 241.8
Description: The Multiple Species Tissue Array (MSTA) slides were designed to study protein expression patterns in different cells and tissues from multiple species. Tissue slices from three different species are mounted on each MSTA slide which can then be treated as a single histological slide for H&E staining, immunohistochemistry, or in situ hybridization. This format allows a rapid analysis of protein expression and localization across different species. MSTA slides can also be used to quickly determine the species reactivity of a given antibody.

Probable Small Intestine Urate Exporter (SLC17A4) Antibody

20-abx312468
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Total Protein from Liver Cirrhosis: Small Intestine

P1236226Lcs 1 mg
EUR 553.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Liver Cirrhosis: Small Intestine

R1236226Lcs-50 50 ug
EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Small intestine carcinoma and normal tissue array

SM801 each
EUR 306
Description: Small intestine carcinoma and normal tissue array, 77 cases/80 cores

Genomic DNA from Mouse Normal Tissue: Small Intestine

D1334226 100 ug
EUR 338.4
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Small Intestine

D1434226 100 ug
EUR 338.4
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Glucose Transporter Type 5, Small Intestine (GLUT5) Antibody

20-abx133767
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Glucose Transporter Type 5, Small Intestine (GLUT5) Antibody

abx430535-200ul 200 ul
EUR 460.8

Total Protein from Human Tumor Tissue: Small Intestine

P1235226 1 mg
EUR 553.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Liver Cirrhosis: Small Intestine: Duodenum

P1236101Lcs 1 mg
EUR 553.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Liver Cirrhosis: Small Intestine: Ileum

P1236227Lcs 1 mg
EUR 553.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Liver Cirrhosis: Small Intestine: Jejunum

P1236230Lcs 1 mg
EUR 553.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Mouse Normal Tissue: Small Intestine

P1334226 1 mg
EUR 256.8
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein from Human Tumor Tissue: Small Intestine

P3235226 0.1 mg
EUR 489.6
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Monkey (Cyno.) Normal Small Intestine Whole tissue lysate

MCL-1205 1 mg
EUR 628.8

Monkey (Rhesus) Normal Small Intestine Whole tissue lysate

MRL-1316 1 mg
EUR 628.8

Human Adult Small Intestine (Normal) Whole tissue lysate

HAL-1308 1 mg
EUR 628.8

Total RNA from Human Tumor Tissue: Small Intestine

R1235226-10 10 ug
EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Small Intestine

R1334226-50 50 ug
EUR 256.8
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Small Intestine

R1434226-50 50 ug
EUR 256.8
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Frozen Tissue Section - Human Adult Normal: Small Intestine

T1234226 5 slides
EUR 418.8
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Adult Normal: Small Intestine

T2234226 5 slides
EUR 253.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

UNASSIGNED

This paper describes a quick and dependable methodology for species detection in meat merchandise primarily based on real-time PCR. It may be utilized for evaluation of in natura or processed meat. The strategy proposed right here can play an vital position in controlling the origin of meat merchandise, guaranteeing their high quality and security for the complete meals industry-producers to shoppers.

A common mini-vector and an annealing of PCR merchandise (APP)-based cloning technique for handy molecular organic manipulations.

Presently, essentially the most extensively used methods for molecular cloning are sticky-end ligation-based cloning, TA cloning, blunt-end ligation-based cloning and ligase-independent cloning. On this examine we have now developed a novel mini-vector pANY1 which may concurrently meet the necessities of all these cloning methods.

As well as, the choice of applicable restriction digestion websites is tough in some instances due to the presence of inside websites. On this examine, an annealing of PCR merchandise (APP)-based sticky-end cloning technique was launched to keep away from this challenge. Moreover, false positives happen throughout molecular cloning, which will increase the workload of isolating constructive clones.

The plasmid pANY1 incorporates a ccdB cassette between a number of cloning websites, which effectively avoids these false positives. Subsequently, this mini-vector ought to function a great tool with large functions in biosciences, agriculture, meals applied sciences.

 

cDNA from Human Adult Normal Tissue: Small Intestine

C1234226 40 reactions
EUR 451.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Diabetic Tissue: Small Intestine: Duodenum

C1236101Dia 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Diabetic Tissue: Small Intestine: Ileum

C1236227Dia 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Diabetic Tissue: Small Intestine: Jejunum

C1236230Dia 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine

C1534226 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine

C1534226-Cy 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Small Intestine: Duodenum

C1234101-10 10 reactions
EUR 277.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Small Intestine: Ileum

C1234227 40 reactions
EUR 451.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Small Intestine: Jejunum

C1234230 40 reactions
EUR 451.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine: Duodenum

C1534101 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine: Duodenum

C1534101-Cy 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine: Ileum

C1534227 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine: Ileum

C1534227-Cy 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine: Jejunum

C1534230 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine: Jejunum

C1534230-Cy 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Small Intestine Lysate

1468 0.1 mg
EUR 229.2
Description: Small Intestine tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Small Intestine Lysate

1308 0.1 mg
EUR 229.2
Description: Small intestine tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Small intestine cancer

SM208 each
EUR 546
Description: Small intestine cancer, metastatic lymph nodes and normal tissue high density tissue microarray, 69 cases/208 core, with stage and grade data

Human Small Intestine Endothelial Cells

HEC15 500,000+ Cells Frozen
EUR 1296

Human Small Intestine Tumor lysate

HTL-1338 1 mg
EUR 927.6

Small intestine disease spectrum (small intestine cancer progression) tissue array

SM802 each
EUR 306
Description: Small intestine disease spectrum (small intestine cancer progression) tissue array, with grade, stage and TNM data, 72 cases/80 cores

Small Intestine Tumor Lysate

1338 0.1 mg
EUR 304.8
Description: Small Intestine tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Small Intestine Cytoplasmic Lysate

XBL-10875 0.1 mg
EUR 273.3
Description: Human small intestine tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human small intestine tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated small intestine tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated small intestine tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

Small Intestine Membrane Lysate

XBL-10876 0.1 mg
EUR 619.8
Description: Human small intestine tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human small intestine tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated small intestine tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated small intestine tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Small intestine Tissue Slide (Normal)

11-801-10um 10 um
EUR 241.8

Small intestine Tissue Slide (Normal)

11-801-4um 4 um
EUR 216.6

Small Intestine Tissue Slide (Malignant)

11-814-10um 10 um
EUR 241.8

Small Intestine Tissue Slide (Malignant)

11-814-4um 4 um
EUR 216.6

Small Intestine Tissue Slide (Adenocarcinoma)

11-815-10um 10 um
EUR 241.8

Small Intestine Tissue Slide (Adenocarcinoma)

11-815-4um 4 um
EUR 216.6

Small Intestine Tissue Slide (Abnormal)

11-824-10um 10 um
EUR 241.8

Small Intestine Tissue Slide (Abnormal)

11-824-4um 4 um
EUR 216.6

Small Intestine Liver Cirrhosis Lysate

XBL-10377 0.1 mg
EUR 796.2
Description: Human small intestine tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human small intestine tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the small intestine tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The small intestine tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Small Intestine Membrane Tumor Lysate

XBL-10888 0.1 mg
EUR 752.1
Description: Human small intestine tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human small intestine tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated small intestine tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated small intestine tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Normal small intestine tissue array

SMN601 each
EUR 222
Description: Normal small intestine tissue array, including TNM, clinical stage and pathology grade, 30 cases/ 60 cores

Mouse Small Intestine Whole tissue lysate

MAL-1408 1 mg
EUR 628.8

Rat Small Intestine Whole tissue lysate

RAL-1468 1 mg
EUR 628.8

Total RNA from Lupus: Small Intestine

R1236226Lup-50 50 ug
EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Small intestine carcinoma test tissue array

T421b each
EUR 48
Description: Small intestine carcinoma test tissue array, with normal small intestine tissues, including pathology grade, TNM and clinical stage, 6 cases/24 cores, replacing T421a

Total Protein from Human Tumor Tissue: Small Intestine

P1235226 1 mg
EUR 553.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein from Human Tumor Tissue: Small Intestine

P3235226 0.1 mg
EUR 489.6
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Human Adult Small Intestine (Normal) Whole tissue lysate

HAL-1308 1 mg
EUR 628.8

Total RNA from Human Tumor Tissue: Small Intestine

R1235226-10 10 ug
EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Frozen Tissue Section - Human Adult Normal: Small Intestine

T1234226 5 slides
EUR 418.8
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Adult Normal: Small Intestine

T2234226 5 slides
EUR 253.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Matched Pair Tissue Slides - Small Intestine Tissue

11-800-MPPT 1 pack
EUR 241.8
Description: The Matched Pair Paraffin Tissue (MPPT) slides are designed for identifying tumor-specific/metastasis genes or proteins. Slices from normal and malignant tissues are mounted on each MPPT slide which can then be treated as a single histological slide for H&E staining, immunohistochemistry, or in situ hybridization. This format allows a rapid analysis of protein expression and localization across normal and cancerous tissue.

Multiple Species Tissue Slides - Small Intestine Tissue

11-800-MSTA 1 pack
EUR 241.8
Description: The Multiple Species Tissue Array (MSTA) slides were designed to study protein expression patterns in different cells and tissues from multiple species. Tissue slices from three different species are mounted on each MSTA slide which can then be treated as a single histological slide for H&E staining, immunohistochemistry, or in situ hybridization. This format allows a rapid analysis of protein expression and localization across different species. MSTA slides can also be used to quickly determine the species reactivity of a given antibody.

Probable Small Intestine Urate Exporter (SLC17A4) Antibody

20-abx312468
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Total Protein from Liver Cirrhosis: Small Intestine

P1236226Lcs 1 mg
EUR 553.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Liver Cirrhosis: Small Intestine

R1236226Lcs-50 50 ug
EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Small intestine carcinoma and normal tissue array

SM801 each
EUR 306
Description: Small intestine carcinoma and normal tissue array, 77 cases/80 cores

Total Protein from Human Adult Normal Tissue: Small Intestine

P1234226 1 mg
EUR 256.8
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Small Intestine: Duodenum

P1235101 1 mg
EUR 553.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Diabetic Tissue: Small Intestine: Duodenum

P1236101Dia 1 mg
EUR 553.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Diabetic Tissue: Small Intestine: Ileum

P1236227Dia 1 mg
EUR 553.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Diabetic Tissue: Small Intestine: Jejunum

P1236230Dia 1 mg
EUR 553.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein from Human Adult Normal Tissue: Small Intestine

P3234226 0.1 mg
EUR 342
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Human Adult Normal Tissue: Small Intestine

R1234226-50 50 ug
EUR 213.6
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Frozen Tissue Section - Human Diabetic Tissue: Small Intestine: Duodenum

T1236101Dia 5 slides
EUR 558
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Frozen Tissue Section - Human Diabetic Tissue: Small Intestine: Ileum

T1236227Dia 5 slides
EUR 558
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Frozen Tissue Section - Human Diabetic Tissue: Small Intestine: Jejunum

T1236230Dia 5 slides
EUR 558
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Small Intestine Tumor: Duodenum Adenocarcinoma

T2235226-1 5 slides
EUR 308.4
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Diabetic Tissue: Small Intestine: Duodenum

T2236101Dia 5 slides
EUR 308.4
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Diabetic Tissue: Small Intestine: Ileum

T2236227Dia 5 slides
EUR 308.4
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Diabetic Tissue: Small Intestine: Jejunum

T2236230Dia 5 slides
EUR 308.4
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Genomic DNA from Mouse Normal Tissue: Small Intestine

D1334226 100 ug
EUR 338.4
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Small Intestine

D1434226 100 ug
EUR 338.4
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Glucose Transporter Type 5, Small Intestine (GLUT5) Antibody

20-abx133767
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Glucose Transporter Type 5, Small Intestine (GLUT5) Antibody

abx430535-200ul 200 ul
EUR 460.8

Total Protein from Liver Cirrhosis: Small Intestine: Duodenum

P1236101Lcs 1 mg
EUR 553.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Liver Cirrhosis: Small Intestine: Ileum

P1236227Lcs 1 mg
EUR 553.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Liver Cirrhosis: Small Intestine: Jejunum

P1236230Lcs 1 mg
EUR 553.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

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