Gene targeting PCR assay for the detection of Crocodylus porosus

Double gene focusing on PCR assay for the detection of Crocodylus porosus in industrial merchandise.

The demand for crocodile meat is rapidly rising due to its unique and organoleptic attraction and likewise the low content material of ldl cholesterol and lipids. Furthermore, crocodile oil and blood have been utilized in various medicines for treating bronchial asthma and several other different illnesses since historical occasions. Moreover, crocodile hides have nice demand in leather-based industries.

 

  • All of those have collectively contributed to the intensive searching, unlawful buying and selling and consequent decline of crocodiles in most elements of the world. To maintain area with the rising calls for, some crocodile species comparable to Crocodylus porosus have been raised in farms and its industrial trades have been legalised.
  • Nevertheless, demand for wild crocodiles in meals and medicines has continued in excessive gear. Not too long ago, a number of DNA-based strategies have been proposed for crocodile detection, however these assays are primarily based on single gene and longer-sized amplicon targets that break down throughout intensive processing.
  • To handle this hole, right here we developed and validated a extremely secure double gene focused multiplex PCR assay for the identification of C. porosus supplies in industrial merchandise. The assay concerned two quick websites from C. porosus atp6 (77 bp) and cytb (127 bp) genes and a common inside management (99 bp) for eukaryotes.
  • The PCR primers had been cross-tested in opposition to 18 species and validated underneath pure and blended matrices underneath intensive boiling, autoclaving and microwave cooking circumstances.
  • Lastly, it was used to determine 5 crocodile-based industrial merchandise. The decrease limits of detection for atp6 and cytb genes had been 0.001 ng and 0.01 ng DNA, respectively, in pure meat and 1% underneath blended matrices.
  • Some inherent options, comparable to 77-127 bp amplicon sizes, distinctive stability and superior sensitivity, prompt the assay could possibly be used for the identification of C. porosus in any forensic specimen.

A Quick and Dependable Actual-Time PCR Technique for Detection of Ten Animal Species in Meat Merchandise.

Species substitution in meat merchandise is a typical drawback reported worldwide. One of these meals fraud is, usually, an intentional act for financial achieve, utilizing sources of low-priced meats in high-value meat merchandise. Penalties embody financial, well being, and non secular issues. Extremely delicate and environment friendly methods are thus required to detect meat species.

 

This paper describes a way primarily based on real-time PCR to detect 10 animal species (Bos taurus, Sus scrofa, Ovis aries, Capra hircus, Gallus gallus, Meleagris gallopavo, Bubalus bubalis, Equus caballus, Felis catus, and Canis familiaris) in meat product. The strategy combines species-specific and common (used right here as inside constructive management) primers, and applies soften curve evaluation for amplicon checking. Technique accuracy was evaluated on 46 experimental meat mixtures and all species had been accurately recognized in all instances, at 1% take a look at sensitivity.

 

Evaluation of 14 industrial meat merchandise revealed that 6 of 14 samples had nondeclared bovine and/or rooster materials. We carried out an interlaboratory comparability utilizing the reference meat mixtures and industrial samples, attaining 100% of reproducibility. The developed take a look at proved to be efficient and dependable for routine evaluation of meat merchandise.

genpcr

genpcr

cDNA from Liver Cirrhosis: Small Intestine: Duodenum

C1236101Lcs 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Small Intestine: Ileum

C1236227Lcs 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Small Intestine: Jejunum

C1236230Lcs 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Mouse Normal Tissue: Small Intestine

C1334226 40 reactions
EUR 540.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Small Intestine

C1434226 40 reactions
EUR 540.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dog Normal Tissue: Small Intestine

C1734226 40 reactions
EUR 376.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Small Intestine

C1234226 40 reactions
EUR 376.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Diabetic Tissue: Small Intestine: Duodenum

C1236101Dia 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Diabetic Tissue: Small Intestine: Ileum

C1236227Dia 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Diabetic Tissue: Small Intestine: Jejunum

C1236230Dia 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine

C1534226 40 reactions
EUR 540.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine

C1534226-Cy 40 reactions
EUR 540.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Small Intestine: Duodenum

C1234101-10 10 reactions
EUR 231.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Small Intestine: Ileum

C1234227 40 reactions
EUR 376.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Small Intestine: Jejunum

C1234230 40 reactions
EUR 376.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine: Duodenum

C1534101 40 reactions
EUR 540.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine: Duodenum

C1534101-Cy 40 reactions
EUR 540.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine: Ileum

C1534227 40 reactions
EUR 540.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine: Ileum

C1534227-Cy 40 reactions
EUR 540.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine: Jejunum

C1534230 40 reactions
EUR 540.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine: Jejunum

C1534230-Cy 40 reactions
EUR 540.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human Small Intestine Tumor lysate

HTL-1338 1 mg
EUR 773.00

Human Small Intestine Endothelial Cells

HEC15 500,000+ Cells Frozen
EUR 1080.00

Mouse Small Intestine Whole tissue lysate

MAL-1408 1 mg
EUR 524.00

Total RNA from Lupus: Small Intestine

R1236226Lup-50 50 ug
EUR 351.00
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Rat Small Intestine Whole tissue lysate

RAL-1468 1 mg
EUR 524.00

Probable Small Intestine Urate Exporter (SLC17A4) Antibody

20-abx312468
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Total Protein from Liver Cirrhosis: Small Intestine

P1236226Lcs 1 mg
EUR 461.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Liver Cirrhosis: Small Intestine

R1236226Lcs-50 50 ug
EUR 351.00
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Glucose Transporter Type 5, Small Intestine (GLUT5) Antibody

20-abx133767
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

Glucose Transporter Type 5, Small Intestine (GLUT5) Antibody

abx430535-200ul 200 ul
EUR 384.00
  • Shipped within 1-3 working days.

Genomic DNA from Mouse Normal Tissue: Small Intestine

D1334226 100 ug
EUR 282.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Small Intestine

D1434226 100 ug
EUR 282.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human Adult Small Intestine (Normal) Whole tissue lysate

HAL-1308 1 mg
EUR 524.00

Monkey (Rhesus) Normal Small Intestine Whole tissue lysate

MRL-1316 1 mg
EUR 524.00

Monkey (Cyno.) Normal Small Intestine Whole tissue lysate

MCL-1205 1 mg
EUR 524.00

Membrane Protein from Human Tumor Tissue: Small Intestine

P3235226 0.1 mg
EUR 408.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Small Intestine

P1235226 1 mg
EUR 461.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Liver Cirrhosis: Small Intestine: Duodenum

P1236101Lcs 1 mg
EUR 461.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Liver Cirrhosis: Small Intestine: Ileum

P1236227Lcs 1 mg
EUR 461.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Liver Cirrhosis: Small Intestine: Jejunum

P1236230Lcs 1 mg
EUR 461.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Mouse Normal Tissue: Small Intestine

P1334226 1 mg
EUR 214.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Human Tumor Tissue: Small Intestine

R1235226-10 10 ug
EUR 351.00
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Small Intestine

R1334226-50 50 ug
EUR 214.00
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Small Intestine

R1434226-50 50 ug
EUR 214.00
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Frozen Tissue Section - Human Adult Normal: Small Intestine

T1234226 5 slides
EUR 349.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Adult Normal: Small Intestine

T2234226 5 slides
EUR 211.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Membrane Protein from Human Adult Normal Tissue: Small Intestine

P3234226 0.1 mg
EUR 285.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Adult Normal Tissue: Small Intestine

P1234226 1 mg
EUR 214.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Tissue: Small Intestine: Duodenum

P1235101 1 mg
EUR 461.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Diabetic Tissue: Small Intestine: Duodenum

P1236101Dia 1 mg
EUR 461.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Diabetic Tissue: Small Intestine: Ileum

P1236227Dia 1 mg
EUR 461.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Diabetic Tissue: Small Intestine: Jejunum

P1236230Dia 1 mg
EUR 461.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Human Adult Normal Tissue: Small Intestine

R1234226-50 50 ug
EUR 178.00
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Monkey (Rhesus) Normal Tissue: Small Intestine

R1534226-50 50 ug
EUR 194.00
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine

R1534226-Cy 50 ug
EUR 194.00
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Frozen Tissue Section - Human Diabetic Tissue: Small Intestine: Duodenum

T1236101Dia 5 slides
EUR 465.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Frozen Tissue Section - Human Diabetic Tissue: Small Intestine: Ileum

T1236227Dia 5 slides
EUR 465.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Frozen Tissue Section - Human Diabetic Tissue: Small Intestine: Jejunum

T1236230Dia 5 slides
EUR 465.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Small Intestine Tumor: Duodenum Adenocarcinoma

T2235226-1 5 slides
EUR 257.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Diabetic Tissue: Small Intestine: Duodenum

T2236101Dia 5 slides
EUR 257.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Diabetic Tissue: Small Intestine: Ileum

T2236227Dia 5 slides
EUR 257.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Diabetic Tissue: Small Intestine: Jejunum

T2236230Dia 5 slides
EUR 257.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Genomic DNA from Lupus: Small Intestine, from a single donor

D1236226Lup 50 ug
EUR 446.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Membrane Protein from Human Adult Normal Tissue: Small Intestine: Duodenum

P3234101 0.1 mg
EUR 285.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein from Human Adult Normal Tissue: Small Intestine: Ileum

P3234227 0.1 mg
EUR 285.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein from Human Adult Normal Tissue: Small Intestine: Jejunum

P3234230 0.1 mg
EUR 285.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

UNASSIGNED

This paper describes a quick and dependable methodology for species detection in meat merchandise primarily based on real-time PCR. It may be utilized for evaluation of in natura or processed meat. The strategy proposed right here can play an vital position in controlling the origin of meat merchandise, guaranteeing their high quality and security for the complete meals industry-producers to shoppers.

A common mini-vector and an annealing of PCR merchandise (APP)-based cloning technique for handy molecular organic manipulations.

Presently, essentially the most extensively used methods for molecular cloning are sticky-end ligation-based cloning, TA cloning, blunt-end ligation-based cloning and ligase-independent cloning. On this examine we have now developed a novel mini-vector pANY1 which may concurrently meet the necessities of all these cloning methods.

As well as, the choice of applicable restriction digestion websites is tough in some instances due to the presence of inside websites. On this examine, an annealing of PCR merchandise (APP)-based sticky-end cloning technique was launched to keep away from this challenge. Moreover, false positives happen throughout molecular cloning, which will increase the workload of isolating constructive clones.

The plasmid pANY1 incorporates a ccdB cassette between a number of cloning websites, which effectively avoids these false positives. Subsequently, this mini-vector ought to function a great tool with large functions in biosciences, agriculture, meals applied sciences.

 

cDNA from Liver Cirrhosis: Small Intestine: Jejunum
C1236230Lcs 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Mouse Normal Tissue: Small Intestine
C1334226 40 reactions
EUR 540.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Rat Normal Tissue: Small Intestine
C1434226 40 reactions
EUR 540.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Dog Normal Tissue: Small Intestine
C1734226 40 reactions
EUR 376.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Human Adult Normal Tissue: Small Intestine
C1234226 40 reactions
EUR 376.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Human Diabetic Tissue: Small Intestine: Duodenum
C1236101Dia 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Human Diabetic Tissue: Small Intestine: Ileum
C1236227Dia 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Human Diabetic Tissue: Small Intestine: Jejunum
C1236230Dia 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine
C1534226 40 reactions
EUR 540.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine
C1534226-Cy 40 reactions
EUR 540.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Human Adult Normal Tissue: Small Intestine: Duodenum
C1234101-10 10 reactions
EUR 231.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Human Adult Normal Tissue: Small Intestine: Ileum
C1234227 40 reactions
EUR 376.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Human Adult Normal Tissue: Small Intestine: Jejunum
C1234230 40 reactions
EUR 376.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine: Duodenum
C1534101 40 reactions
EUR 540.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine: Duodenum
C1534101-Cy 40 reactions
EUR 540.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine: Ileum
C1534227 40 reactions
EUR 540.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine: Ileum
C1534227-Cy 40 reactions
EUR 540.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Rhesus) Normal Tissue: Small Intestine: Jejunum
C1534230 40 reactions
EUR 540.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine: Jejunum
C1534230-Cy 40 reactions
EUR 540.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Human Small Intestine Tumor lysate
HTL-1338 1 mg
EUR 773.00
Human Small Intestine Endothelial Cells
HEC15 500,000+ Cells Frozen
EUR 1080.00
Mouse Small Intestine Whole tissue lysate
MAL-1408 1 mg
EUR 524.00
Total RNA from Lupus: Small Intestine
R1236226Lup-50 50 ug
EUR 351.00
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Rat Small Intestine Whole tissue lysate
RAL-1468 1 mg
EUR 524.00
Human Adult Small Intestine (Normal) Whole tissue lysate
HAL-1308 1 mg
EUR 524.00
Membrane Protein from Human Tumor Tissue: Small Intestine
P3235226 0.1 mg
EUR 408.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Human Tumor Tissue: Small Intestine
P1235226 1 mg
EUR 461.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total RNA from Human Tumor Tissue: Small Intestine
R1235226-10 10 ug
EUR 351.00
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Frozen Tissue Section - Human Adult Normal: Small Intestine
T1234226 5 slides
EUR 349.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section - Human Adult Normal: Small Intestine
T2234226 5 slides
EUR 211.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Probable Small Intestine Urate Exporter (SLC17A4) Antibody
20-abx312468
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.
Total Protein from Liver Cirrhosis: Small Intestine
P1236226Lcs 1 mg
EUR 461.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total RNA from Liver Cirrhosis: Small Intestine
R1236226Lcs-50 50 ug
EUR 351.00
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Membrane Protein from Human Adult Normal Tissue: Small Intestine
P3234226 0.1 mg
EUR 285.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Human Adult Normal Tissue: Small Intestine
P1234226 1 mg
EUR 214.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Human Tumor Tissue: Small Intestine: Duodenum
P1235101 1 mg
EUR 461.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Human Diabetic Tissue: Small Intestine: Duodenum
P1236101Dia 1 mg
EUR 461.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Human Diabetic Tissue: Small Intestine: Ileum
P1236227Dia 1 mg
EUR 461.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Human Diabetic Tissue: Small Intestine: Jejunum
P1236230Dia 1 mg
EUR 461.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total RNA from Human Adult Normal Tissue: Small Intestine
R1234226-50 50 ug
EUR 178.00
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Frozen Tissue Section - Human Diabetic Tissue: Small Intestine: Duodenum
T1236101Dia 5 slides
EUR 465.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Frozen Tissue Section - Human Diabetic Tissue: Small Intestine: Ileum
T1236227Dia 5 slides
EUR 465.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Frozen Tissue Section - Human Diabetic Tissue: Small Intestine: Jejunum
T1236230Dia 5 slides
EUR 465.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section - Human Small Intestine Tumor: Duodenum Adenocarcinoma
T2235226-1 5 slides
EUR 257.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section - Human Diabetic Tissue: Small Intestine: Duodenum
T2236101Dia 5 slides
EUR 257.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section - Human Diabetic Tissue: Small Intestine: Ileum
T2236227Dia 5 slides
EUR 257.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section - Human Diabetic Tissue: Small Intestine: Jejunum
T2236230Dia 5 slides
EUR 257.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Glucose Transporter Type 5, Small Intestine (GLUT5) Antibody
20-abx133767
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
Glucose Transporter Type 5, Small Intestine (GLUT5) Antibody
abx430535-200ul 200 ul
EUR 384.00
  • Shipped within 1-3 working days.
Genomic DNA from Mouse Normal Tissue: Small Intestine
D1334226 100 ug
EUR 282.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Genomic DNA from Rat Normal Tissue: Small Intestine
D1434226 100 ug
EUR 282.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Monkey (Rhesus) Normal Small Intestine Whole tissue lysate
MRL-1316 1 mg
EUR 524.00
Monkey (Cyno.) Normal Small Intestine Whole tissue lysate
MCL-1205 1 mg
EUR 524.00
Total Protein from Liver Cirrhosis: Small Intestine: Duodenum
P1236101Lcs 1 mg
EUR 461.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Liver Cirrhosis: Small Intestine: Ileum
P1236227Lcs 1 mg
EUR 461.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Liver Cirrhosis: Small Intestine: Jejunum
P1236230Lcs 1 mg
EUR 461.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Mouse Normal Tissue: Small Intestine
P1334226 1 mg
EUR 214.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total RNA from Mouse Normal Tissue: Small Intestine
R1334226-50 50 ug
EUR 214.00
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total RNA from Rat Normal Tissue: Small Intestine
R1434226-50 50 ug
EUR 214.00
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Membrane Protein from Human Adult Normal Tissue: Small Intestine: Duodenum
P3234101 0.1 mg
EUR 285.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Membrane Protein from Human Adult Normal Tissue: Small Intestine: Ileum
P3234227 0.1 mg
EUR 285.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Membrane Protein from Human Adult Normal Tissue: Small Intestine: Jejunum
P3234230 0.1 mg
EUR 285.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Human Adult Normal Tissue: Small Intestine: Duodenum
P1234101 1 mg
EUR 214.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Human Adult Normal Tissue: Small Intestine: Ileum
P1234227 1 mg
EUR 214.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Human Adult Normal Tissue: Small Intestine: Jejunum
P1234230 1 mg
EUR 214.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total RNA from Human Adult Normal Tissue: Small Intestine: Duodenum
R1234101-50 50 ug
EUR 178.00
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total RNA from Human Adult Normal Tissue: Small Intestine: Ileum
R1234227-50 50 ug
EUR 178.00
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total RNA from Human Adult Normal Tissue: Small Intestine: Jejunum
R1234230-50 50 ug
EUR 178.00
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Human, Mouse, Rat - Small Intestine Tissue Array (5 slides/pk)
TAS-1020 1 pk
EUR 286.00
Total RNA from Monkey (Rhesus) Normal Tissue: Small Intestine
R1534226-50 50 ug
EUR 194.00
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total RNA from Monkey (Cynomolgus) Normal Tissue: Small Intestine
R1534226-Cy 50 ug
EUR 194.00
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Human Small Intestine Tissue Slide (Squamous Cell Carcinoma) (5 slides/pk)
HTS-11801 1 pk
EUR 286.00

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