Utility of droplet digital PCR for the detection of vector copy quantity in scientific CAR/TCR T cell merchandise.
Genetically engineered T cells have turn into an essential remedy for B-cell malignancies. Measuring the effectivity of vector integration into the T cell genome is essential for assessing the efficiency and security of those most cancers immunotherapies.A digital droplet polymerase chain response (ddPCR) assay was developed and evaluated for assessing the common variety of lenti- and retroviral vectors built-in into Chimeric Antigen Receptor (CAR) and T Cell Receptor (TCR)-engineered T cells.
- The ddPCR assay constantly measured the focus of an empty vector in answer and the common variety of CAR and TCR vectors built-in into T cell populations.
- There was a linear relationship between the common vector copy quantity per cell measured by ddPCR and the proportion of cells transduced as measured by stream cytometry.
- Related vector copy quantity measurements had been obtained by completely different employees utilizing the ddPCR assay, highlighting the assays reproducibility amongst technicians. Evaluation of contemporary and cryopreserved CAR T and TCR engineered T cells yielded comparable outcomes.
- ddPCR is a sturdy software for correct quantitation of common vector copy quantity in CAR and TCR engineered T cells. The assay can be relevant to different kinds of genetically engineered cells together with Pure Killer cells and hematopoietic stem cells.
Improvement of a PCR-based lateral stream strip assay for the straightforward, fast, and correct detection of pork in meat and meat merchandise.
In recent times, adulteration of meat and meat merchandise has turn into a significant meals security problem. PCR and real-time PCR applied sciences are mainstream strategies used to determine animal-derived elements.
Nonetheless, these applied sciences rely extremely on pricey gear {and professional} technicians; they’re due to this fact tough to make use of in resource-limited settings. On this research, a novel, extremely delicate molecular assay, Pig-PCR-Strip (Pig particular polymerase chain reaction-Lateral stream strip), was developed for fast detection of pig and swine-derived elements.
The assay relies on PCR amplification, hybridization of the PCR product to the probe, adopted by detection utilizing a strip format. Utilizing this format, the PCR product might be detected by the bare eye inside three min, and gives a foundation for the migration of species-specific detection to a point-of-care (POC) microfluidic format.
The Pig-PCR-Strip can detect pork elements at a focus of 0.01% in adulterated meat, and the restrict of detection is as much as 10 fg of goal DNA. The assay was particular to pork and didn’t cross-react with different non-target species. It additionally can be utilized for industrial samples and sophisticated meals samples.
It’s a promising new software for detection of pig-derived meat and might be quickly modified for figuring out different species. It might be extensively used for fixing issues associated to meat high quality assurance, species authentication, and traceability.
genpcr
 CP-640186 |
|
B3585-10 |
ApexBio |
10 mg |
EUR 331.2 |
CP-640186 |
|
B3585-2 |
ApexBio |
2 mg |
EUR 170.4 |
CP-640186 |
|
B3585-5 |
ApexBio |
5 mg |
EUR 226.8 |
CP-640186 |
|
B3585-50 |
ApexBio |
50 mg |
EUR 1131.6 |
 CP 316819 |
|
B5448-10 |
ApexBio |
10 mg |
EUR 486 |
CP 316819 |
|
B5448-50 |
ApexBio |
50 mg |
EUR 1802.4 |
 CP 20961 |
|
B5474-10 |
ApexBio |
10 mg |
EUR 408 |
CP 20961 |
|
B5474-50 |
ApexBio |
50 mg |
EUR 1531.2 |
 Coelenterazine cp |
|
10112 |
Biotium |
50uG |
EUR 134.4 |
|
Description: Minimum order quantity: 1 unit of 50uG |
Coelenterazine cp |
|
10112-1 |
Biotium |
1MG |
EUR 870 |
|
Description: Minimum order quantity: 1 unit of 1MG |
Coelenterazine cp |
|
10112-2 |
Biotium |
250uG |
EUR 310.8 |
|
Description: Minimum order quantity: 1 unit of 250uG |
 CP 80633 |
|
27301 |
BPS Bioscience |
10 mg |
EUR 310 |
|
Description: CP 80633 is a selective inhibitor of PDE4, which does not display significant isozyme selectivity. It also inhibits hydrolysis of cAMP in isolated monocytes, eosinophils, human peripheral blood and T-cells. It has been shown to exhibit anti-inflammatory and bronchodilatory activity in vivo. |
CP 80633 |
|
B7162-10 |
ApexBio |
10 mg |
EUR 447.6 |
CP 80633 |
|
B7162-50 |
ApexBio |
50 mg |
EUR 1635.6 |
 CP 154526 |
|
B7185-1 |
ApexBio |
1 mg |
EUR 130.8 |
CP 154526 |
|
B7185-5 |
ApexBio |
5 mg |
EUR 176.4 |
 CP 96345 |
|
B7213-10 |
ApexBio |
10 mg |
EUR 466.8 |
CP 96345 |
|
B7213-50 |
ApexBio |
50 mg |
EUR 1771.2 |
 CP 135807 |
|
B7497-10 |
ApexBio |
10 mg |
EUR 447.6 |
CP 135807 |
|
B7497-50 |
ApexBio |
50 mg |
EUR 1635.6 |
 CP 775146 |
|
B7609-10 |
ApexBio |
10 mg |
EUR 525.6 |
CP 775146 |
|
B7609-50 |
ApexBio |
50 mg |
EUR 1966.8 |
 CP-809101 |
|
A3330-10 |
ApexBio |
10 mg |
EUR 301.2 |
|
Description: CP-809101 is a potent and selective 5-HT2C receptor agonist (pEC50 values are 9.96, 7.19 and 6.81 for human 5-HT2C, 5-HT2B and 5-HT2A receptors respectively). |
CP-809101 |
|
A3330-50 |
ApexBio |
50 mg |
EUR 1101.6 |
|
Description: CP-809101 is a potent and selective 5-HT2C receptor agonist (pEC50 values are 9.96, 7.19 and 6.81 for human 5-HT2C, 5-HT2B and 5-HT2A receptors respectively). |
 CP-724714 |
|
A2412-100 |
ApexBio |
100 mg |
EUR 1173.6 |
|
Description: CP-724714 is an inhibitor of erbB2 and EGFR kinases with IC50 values of 10±3 nmol/L and 6,400±2,100 nmol/L, respectively [1].In the in vitro cell cycle assay, CP-724714 cause a G1 block of the Her2-amplified BT-474 breast cancer cells due to its inhibition of erbB2. |
CP-724714 |
|
A2412-25 |
ApexBio |
25 mg |
EUR 505.2 |
|
Description: CP-724714 is an inhibitor of erbB2 and EGFR kinases with IC50 values of 10±3 nmol/L and 6,400±2,100 nmol/L, respectively [1].In the in vitro cell cycle assay, CP-724714 cause a G1 block of the Her2-amplified BT-474 breast cancer cells due to its inhibition of erbB2. |
CP-724714 |
|
A2412-5 |
ApexBio |
5 mg |
EUR 177.6 |
|
Description: CP-724714 is an inhibitor of erbB2 and EGFR kinases with IC50 values of 10±3 nmol/L and 6,400±2,100 nmol/L, respectively [1].In the in vitro cell cycle assay, CP-724714 cause a G1 block of the Her2-amplified BT-474 breast cancer cells due to its inhibition of erbB2. |
CP-724714 |
|
A2412-5.1 |
ApexBio |
10 mM (in 1mL DMSO) |
EUR 196.8 |
|
Description: CP-724714 is an inhibitor of erbB2 and EGFR kinases with IC50 values of 10±3 nmol/L and 6,400±2,100 nmol/L, respectively [1].In the in vitro cell cycle assay, CP-724714 cause a G1 block of the Her2-amplified BT-474 breast cancer cells due to its inhibition of erbB2. |
 CP 471474 |
|
A4435-10 |
ApexBio |
10 mg |
EUR 369.6 |
|
Description: Broad spectrum MMP inhibitor (IC50 values are 0.7, 0.9, 13, 16 and 1170 nM for MMP-2, MMP-13, MMP-9, MMP-3 and MMP-1 respectively). Attenuates early left ventricular dilation after experimental myocardial infarction in mice. |
CP 471474 |
|
A4435-50 |
ApexBio |
50 mg |
EUR 1363.2 |
|
Description: Broad spectrum MMP inhibitor (IC50 values are 0.7, 0.9, 13, 16 and 1170 nM for MMP-2, MMP-13, MMP-9, MMP-3 and MMP-1 respectively). Attenuates early left ventricular dilation after experimental myocardial infarction in mice. |
 CP Antibody |
|
46543-100ul |
SAB |
100ul |
EUR 302.4 |
CP Antibody |
|
45875-100ul |
SAB |
100ul |
EUR 302.4 |
CP Antibody |
|
45875-50ul |
SAB |
50ul |
EUR 224.4 |
 CP-466722 |
|
A8625-10 |
ApexBio |
10 mg |
EUR 273.6 |
|
Description: CP-466722 is a selective inhibitor of ATM kinase with IC50 value of 0.41 ?M [1].ATM (ataxia-telangiectasia, mutated) is a serine/threonine protein kinase and plays an important role in the cellular responses to DNA double-strand breaks (DSBs) [2] [3]. |
CP-466722 |
|
A8625-5 |
ApexBio |
5 mg |
EUR 199.2 |
|
Description: CP-466722 is a selective inhibitor of ATM kinase with IC50 value of 0.41 ?M [1].ATM (ataxia-telangiectasia, mutated) is a serine/threonine protein kinase and plays an important role in the cellular responses to DNA double-strand breaks (DSBs) [2] [3]. |
CP-466722 |
|
A8625-50 |
ApexBio |
50 mg |
EUR 649.2 |
|
Description: CP-466722 is a selective inhibitor of ATM kinase with IC50 value of 0.41 ?M [1].ATM (ataxia-telangiectasia, mutated) is a serine/threonine protein kinase and plays an important role in the cellular responses to DNA double-strand breaks (DSBs) [2] [3]. |
 CP-91149 |
|
A8403-10 |
ApexBio |
10 mg |
EUR 205.2 |
|
Description: CP-91149 is a selective inhibitor of glycogen phosphorylase (GP) with an IC50 value of 0.13 ?M. |
CP-91149 |
|
A8403-25 |
ApexBio |
25 mg |
EUR 346.8 |
|
Description: CP-91149 is a selective inhibitor of glycogen phosphorylase (GP) with an IC50 value of 0.13 ?M. |
CP-91149 |
|
A8403-5 |
ApexBio |
5 mg |
EUR 141.6 |
|
Description: CP-91149 is a selective inhibitor of glycogen phosphorylase (GP) with an IC50 value of 0.13 ?M. |
CP Antibody |
|
1-CSB-PA14599A0Rb |
Cusabio |
|
|
|
|
|
Description: A polyclonal antibody against CP. Recognizes CP from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200 |
CP Antibody |
|
F50324-0.4ML |
NSJ Bioreagents |
0.4 ml |
EUR 322.15 |
|
Description: Ceruloplasmin is a metalloprotein that binds most of the copper in plasma and is involved in the peroxidation of Fe(II) transferrin to Fe(III) transferrin. Mutations in this protein cause aceruloplasminemia, which results in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities. |
Improvement and validation of a fast package for authenticity of murine meat in meat merchandise with a species-specific PCR assay.
Adulteration of meat merchandise with murine meat poses an enormous risk to shopper well being and results in critical disruption in meals markets. Species authentication of murine meat continues to be technically difficult.
We, due to this fact, developed a species-specific PCR package consisting of murine meat DNA extraction, PCR response and figuring out techniques. We designed novel common primers focusing on extremely conserved area on mitochondrial cytochrome b gene (cyt b) from 4 murines (lab rats, lab mice, wild rat and wild mice), in addition to particular primers for meat from 4 extensively consumed animal species, cattle, sheep, duck and donkey.
Concurrently, pasmid inserted particular cyt b fragment was cloned and used as the interior positve management within the package. The package parameters of specificity, sensitivity, stability and validity had been decided utilizing mimic counterfeiting meatball.
The specificity of the DNA detection package was 100% in authentication of the 4 fraudulent meats of cattle, sheep, duck and donkey combined murine meat. The minimal detection restrict of the pattern DNA was 0.1 μg. The package, which had freeze-thawed as much as 20 instances and saved for 1 12 months, additionally was highly effective in detecting an quantity of 0.1 mg in synthetic counterfeited cattle, sheep, duck and donkey meat merchandise.
The murine-species DNA detection package proposed on this research has proved to be a easy, correct and efficient assay, and might be utilized to the identification of murine meat traces in widespread edible meat, to make sure the realisable implementation of meat product market supervision
 Anti-GPR100 Antibody |
|
A09371 |
BosterBio |
100ul |
EUR 476.4 |
|
Description: Rabbit Polyclonal Antibody for GPR100 Antibody (RXFP4) detection.tested for WB in Human. |
 Anti-GPR100 antibody |
|
STJ93312 |
St John's Laboratory |
200 µl |
EUR 236.4 |
|
Description: Rabbit polyclonal to GPR100. |
 anti- GPR105 antibody |
|
FNab03588 |
FN Test |
100µg |
EUR 658.5 |
|
|
|
Description: Antibody raised against GPR105 |
 anti- GPR107 antibody |
|
FNab03589 |
FN Test |
100µg |
EUR 702 |
|
|
|
Description: Antibody raised against GPR107 |
 anti- GPR108 antibody |
|
FNab03590 |
FN Test |
100µg |
EUR 658.5 |
|
|
|
Description: Antibody raised against GPR108 |
 anti- GPR116 antibody |
|
FNab03591 |
FN Test |
100µg |
EUR 658.5 |
|
|
|
Description: Antibody raised against GPR116 |
 anti- GPR132 antibody |
|
FNab03593 |
FN Test |
100µg |
EUR 606.3 |
|
|
|
Description: Antibody raised against GPR132 |
 anti- GPR137 antibody |
|
FNab03594 |
FN Test |
100µg |
EUR 606.3 |
|
|
|
Description: Antibody raised against GPR137 |
 anti- GPR142 antibody |
|
FNab03597 |
FN Test |
100µg |
EUR 702 |
|
|
|
Description: Antibody raised against GPR142 |
 anti- GPR161 antibody |
|
FNab03598 |
FN Test |
100µg |
EUR 606.3 |
|
|
|
Description: Antibody raised against GPR161 |
 anti- GPR162 antibody |
|
FNab03599 |
FN Test |
100µg |
EUR 702 |
|
|
|
Description: Antibody raised against GPR162 |
 anti- GPR177 antibody |
|
FNab03601 |
FN Test |
100µg |
EUR 658.5 |
|
|
|
Description: Antibody raised against GPR177 |
 anti- GPR137B antibody |
|
FNab03595 |
FN Test |
100µg |
EUR 702 |
|
|
|
Description: Antibody raised against GPR137B |
 anti- GPR17 antibody |
|
FNab03600 |
FN Test |
100µg |
EUR 658.5 |
|
|
|
Description: Antibody raised against GPR17 |
 anti- GPR22 antibody |
|
FNab03603 |
FN Test |
100µg |
EUR 606.3 |
|
|
|
Description: Antibody raised against GPR22 |
 anti- GPR35 antibody |
|
FNab03605 |
FN Test |
100µg |
EUR 702 |
|
|
|
Description: Antibody raised against GPR35 |
 anti- GPR50 antibody |
|
FNab03607 |
FN Test |
100µg |
EUR 658.5 |
|
|
|
Description: Antibody raised against GPR50 |
anti- GPR50 antibody |
|
FNab03608 |
FN Test |
100µg |
EUR 658.5 |
|
|
|
Description: Antibody raised against GPR50 |
 anti- GPR81 antibody |
|
FNab03610 |
FN Test |
100µg |
EUR 702 |
|
|
|
Description: Antibody raised against GPR81 |
 anti- GPR83 antibody |
|
FNab03611 |
FN Test |
100µg |
EUR 702 |
|
|
|
Description: Antibody raised against GPR83 |
 anti- GPR183/EBI2 antibody |
|
FNab03602 |
FN Test |
100µg |
EUR 658.5 |
|
|
|
Description: Antibody raised against GPR183/EBI2 |
 anti- GPR3 antibody |
|
FNab03604 |
FN Test |
100µg |
EUR 702 |
|
|
|
Description: Antibody raised against GPR3 |
 Anti-OR51E1/Gpr164 Antibody |
|
A30874 |
BosterBio |
100ul |
EUR 476.4 |
|
Description: Rabbit Polyclonal OR51E1/Gpr164 Antibody. Validated in IF, WB and tested in Human. |
 anti- GPR101-Specific antibody |
|
FNab03587 |
FN Test |
100µg |
EUR 702 |
|
|
|
Description: Antibody raised against GPR101-Specific |
 anti- GPR123-Specific antibody |
|
FNab03592 |
FN Test |
100µg |
EUR 702 |
|
|
|
Description: Antibody raised against GPR123-Specific |
 anti- GPR139-Specific antibody |
|
FNab03596 |
FN Test |
100µg |
EUR 606.3 |
|
|
|
Description: Antibody raised against GPR139-Specific |
 Anti-FFAR3/Gpr41 Antibody |
|
A02437 |
BosterBio |
100ul |
EUR 476.4 |
|
Description: Rabbit Polyclonal FFAR3/Gpr41 Antibody. Validated in IF and tested in Human, Mouse. |
 anti- GPR58/TAAR2 antibody |
|
FNab03609 |
FN Test |
100µg |
EUR 658.5 |
|
|
|
Description: Antibody raised against GPR58/TAAR2 |
 Anti-SUCNR1/Gpr91 Antibody |
|
A08485 |
BosterBio |
100ul |
EUR 476.4 |
|
Description: Rabbit Polyclonal SUCNR1/Gpr91 Antibody. Validated in WB and tested in Human, Mouse, Rat. |
 anti- GPR37/Pael-R antibody |
|
FNab03606 |
FN Test |
100µg |
EUR 606.3 |
|
|
|
Description: Antibody raised against GPR37/Pael-R |
 Anti-GPCR GPR40/FFAR1 Antibody |
|
A01349-2 |
BosterBio |
100ug/vial |
EUR 352.8 |
 Anti-LGR4/Gpr48 Monoclonal Antibody |
|
M02134 |
BosterBio |
100uL |
EUR 531.6 |
|
Description: Mouse Monoclonal LGR4/Gpr48 Antibody. Validated in IHC, WB and tested in Human. |
 Polyclonal Goat Anti-TAS1R2 / GPR71 Antibody |
|
AMM05957G |
Leading Biology |
0.1 mg |
EUR 580.8 |
|
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-TAS1R2 / GPR71 . This antibody is tested and proven to work in the following applications: |
 Anti-LGR5/GPR49 Rabbit Monoclonal Antibody |
|
M00239 |
BosterBio |
100ug/vial |
EUR 476.4 |
|
Description: Rabbit Monoclonal LGR5/GPR49 Antibody. Validated in Flow Cytometry, IP, WB and tested in Human, Mouse, Rat. |
 Anti-GPCR GPR7/NPBWR1 Antibody |
|
A08247-1 |
BosterBio |
100ug/vial |
EUR 352.8 |
) Anti Human GPR120 Monoclonal Antibody (Clone No. 2B6) |
|
KG138 |
Sceti |
each |
EUR 856.8 |
|
Description: The Anti Human GPR120 Monoclonal Antibody (Clone No. 2B6) is available in Europe and for worldwide shipping via Gentaur. |
) Anti Mouse GPR40 Monoclonal Antibody (Clone No. G16) |
|
KG116 |
Sceti |
each |
EUR 919.2 |
|
Description: The Anti Mouse GPR40 Monoclonal Antibody (Clone No. G16) is available in Europe and for worldwide shipping via Gentaur. |
 Polyclonal Antibody) Rabbit Anti Human Gpr15 (N-Terminal) Polyclonal Antibody |
|
CPBT-65692RH |
Creative Diagnostics |
0.1 mg |
EUR 696 |
 anti- GPRIN1 antibody |
|
FNab03615 |
FN Test |
100µg |
EUR 658.5 |
|
|
|
Description: Antibody raised against GPRIN1 |
 anti- GPRASP2 antibody |
|
FNab03613 |
FN Test |
100µg |
EUR 658.5 |
|
|
|
Description: Antibody raised against GPRASP2 |
 Anti-PPP2R3C/G5Pr Antibody |
|
A11897 |
BosterBio |
100ul |
EUR 476.4 |
|
Description: Rabbit Polyclonal PPP2R3C/G5Pr Antibody. Validated in WB and tested in Human. |
Anti-PPP2R3C/G5Pr Antibody |
|
A11897-1 |
BosterBio |
100ul |
EUR 476.4 |
|
Description: Rabbit Polyclonal Antibody for PPP2R3C Antibody (PPP2R3C) detection. Tested with WB in Human, Mouse, Rat. |
 Antibody) GPR105 (GPR105) Antibody |
|
abx233588-100ug |
Abbexa |
100 ug |
EUR 610.8 |
|
|
GPR105 (GPR105) Antibody |
|
20-abx217588 |
Abbexa |
|
|
|
|
GPR105 (GPR105) Antibody |
|
20-abx008376 |
Abbexa |
-
EUR 360.00
-
EUR 526.80
-
EUR 226.80
|
|
|
|
 GPR174 antibody |
|
70R-10017 |
Fitzgerald |
50 ug |
EUR 560.4 |
|
Description: Affinity purified rabbit polyclonal GPR174 antibody |
 GPR164 antibody |
|
70R-12526 |
Fitzgerald |
100 ul |
EUR 548.4 |
|
Description: Affinity purified Rabbit polyclonal GPR164 antibody |
 GPR120 antibody |
|
70R-12527 |
Fitzgerald |
100 ul |
EUR 548.4 |
|
Description: Affinity purified Rabbit polyclonal GPR120 antibody |
 GPR114 antibody |
|
70R-12939 |
Fitzgerald |
100 ul |
EUR 548.4 |
|
Description: Affinity purified Rabbit polyclonal GPR114 antibody |
 GPR139 Antibody |
|
44331-100ul |
SAB |
100ul |
EUR 302.4 |
GPR139 Antibody |
|
44331-50ul |
SAB |
50ul |
EUR 224.4 |
 GPR143 Antibody |
|
47887-100ul |
SAB |
100ul |
EUR 302.4 |
 GPR123 Antibody |
|
47892-100ul |
SAB |
100ul |
EUR 302.4 |
 GPR115 Antibody |
|
47967-100ul |
SAB |
100ul |
EUR 302.4 |
 GPR103 Antibody |
|
47988-100ul |
SAB |
100ul |
EUR 302.4 |
 GPR151 Antibody |
|
47989-100ul |
SAB |
100ul |
EUR 302.4 |
 GPR169 Antibody |
|
47991-100ul |
SAB |
100ul |
EUR 302.4 |
 GPR132 Antibody |
|
48165-100ul |
SAB |
100ul |
EUR 399.6 |
GPR132 Antibody |
|
48165-50ul |
SAB |
50ul |
EUR 286.8 |
GPR132 Antibody |
|
47590-100ul |
SAB |
100ul |
EUR 302.4 |
 GPR137 Antibody |
|
47602-100ul |
SAB |
100ul |
EUR 302.4 |
 GPR149 Antibody |
|
47603-100ul |
SAB |
100ul |
EUR 302.4 |
 GPR160 Antibody |
|
47605-100ul |
SAB |
100ul |
EUR 302.4 |
 GPR153 Antibody |
|
47606-100ul |
SAB |
100ul |
EUR 302.4 |
GPR151 Antibody |
|
47770-100ul |
SAB |
100ul |
EUR 302.4 |
 GPR142 Antibody |
|
47778-100ul |
SAB |
100ul |
EUR 302.4 |
 GPR157 Antibody |
|
47779-100ul |
SAB |
100ul |
EUR 302.4 |
 GPR180 Antibody |
|
47826-100ul |
SAB |
100ul |
EUR 302.4 |
 GPR106 Antibody |
|
47853-100ul |
SAB |
100ul |
EUR 302.4 |
 GPR112 Antibody |
|
44576-100ul |
SAB |
100ul |
EUR 302.4 |
GPR112 Antibody |
|
44576-50ul |
SAB |
50ul |
EUR 224.4 |
 GPR125 Antibody |
|
44577-100ul |
SAB |
100ul |
EUR 302.4 |
GPR125 Antibody |
|
44577-50ul |
SAB |
50ul |
EUR 224.4 |
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