Utility of droplet digital PCR for the detection of vector copy quantity in scientific CAR/TCR T cell merchandise.
Genetically engineered T cells have turn into an essential remedy for B-cell malignancies. Measuring the effectivity of vector integration into the T cell genome is essential for assessing the efficiency and security of those most cancers immunotherapies.A digital droplet polymerase chain response (ddPCR) assay was developed and evaluated for assessing the common variety of lenti- and retroviral vectors built-in into Chimeric Antigen Receptor (CAR) and T Cell Receptor (TCR)-engineered T cells.
- The ddPCR assay constantly measured the focus of an empty vector in answer and the common variety of CAR and TCR vectors built-in into T cell populations.
- There was a linear relationship between the common vector copy quantity per cell measured by ddPCR and the proportion of cells transduced as measured by stream cytometry.
- Related vector copy quantity measurements had been obtained by completely different employees utilizing the ddPCR assay, highlighting the assays reproducibility amongst technicians. Evaluation of contemporary and cryopreserved CAR T and TCR engineered T cells yielded comparable outcomes.
- ddPCR is a sturdy software for correct quantitation of common vector copy quantity in CAR and TCR engineered T cells. The assay can be relevant to different kinds of genetically engineered cells together with Pure Killer cells and hematopoietic stem cells.
Improvement of a PCR-based lateral stream strip assay for the straightforward, fast, and correct detection of pork in meat and meat merchandise.
In recent times, adulteration of meat and meat merchandise has turn into a significant meals security problem. PCR and real-time PCR applied sciences are mainstream strategies used to determine animal-derived elements.
Nonetheless, these applied sciences rely extremely on pricey gear {and professional} technicians; they’re due to this fact tough to make use of in resource-limited settings. On this research, a novel, extremely delicate molecular assay, Pig-PCR-Strip (Pig particular polymerase chain reaction-Lateral stream strip), was developed for fast detection of pig and swine-derived elements.
The assay relies on PCR amplification, hybridization of the PCR product to the probe, adopted by detection utilizing a strip format. Utilizing this format, the PCR product might be detected by the bare eye inside three min, and gives a foundation for the migration of species-specific detection to a point-of-care (POC) microfluidic format.
The Pig-PCR-Strip can detect pork elements at a focus of 0.01% in adulterated meat, and the restrict of detection is as much as 10 fg of goal DNA. The assay was particular to pork and didn’t cross-react with different non-target species. It additionally can be utilized for industrial samples and sophisticated meals samples.
It’s a promising new software for detection of pig-derived meat and might be quickly modified for figuring out different species. It might be extensively used for fixing issues associated to meat high quality assurance, species authentication, and traceability.
genpcr
 CP-724714 |
|
A2412-5 |
ApexBio |
5 mg |
EUR 68 |
|
|
|
Description: JAK/STAT Signaling|EGFR#Tyrosine Kinase|EGFR#Tyrosine Kinase|HER2 |
CP-724714 |
|
A2412-5.1 |
ApexBio |
10 mM (in 1mL DMSO) |
EUR 73.6 |
|
|
|
Description: JAK/STAT Signaling|EGFR#Tyrosine Kinase|EGFR#Tyrosine Kinase|HER2 |
 CP 471474 |
|
A4435-1 |
ApexBio |
1mg |
EUR 40 |
|
|
|
Description: Proteases|MMP |
CP 471474 |
|
A4435-10 |
ApexBio |
10 mg |
EUR 176 |
|
|
|
Description: Proteases|MMP |
CP 471474 |
|
A4435-5 |
ApexBio |
5mg |
EUR 104 |
|
|
|
Description: Proteases|MMP |
CP 471474 |
|
A4435-50 |
ApexBio |
50 mg |
EUR 842 |
|
|
|
Description: Broad spectrum MMP inhibitor |
 CP-809101 |
|
A3330-10 |
ApexBio |
10 mg |
EUR 137.6 |
|
|
|
Description: Neuroscience|5-HT Receptor |
CP-809101 |
|
A3330-50 |
ApexBio |
50 mg |
EUR 591.2 |
|
|
|
Description: Neuroscience|5-HT Receptor |
 CP-91149 |
|
A8403-10 |
ApexBio |
10 mg |
EUR 104.8 |
|
|
|
Description: Metabolism|Phospholipase |
CP-91149 |
|
A8403-25 |
ApexBio |
25 mg |
EUR 210.4 |
|
|
|
Description: Metabolism|Phospholipase |
CP-91149 |
|
A8403-5 |
ApexBio |
5 mg |
EUR 88 |
|
|
|
Description: Metabolism|Phospholipase |
 CP-466722 |
|
A8625-10 |
ApexBio |
10 mg |
EUR 136 |
|
|
|
Description: Cell Cycle/Checkpoint|ATM/ATR#DNA Damage/DNA Repair|ATM/ATR |
CP-466722 |
|
A8625-5 |
ApexBio |
5 mg |
EUR 88 |
|
|
|
Description: Cell Cycle/Checkpoint|ATM/ATR#DNA Damage/DNA Repair|ATM/ATR |
CP-466722 |
|
A8625-50 |
ApexBio |
50 mg |
EUR 373.6 |
|
|
|
Description: Cell Cycle/Checkpoint|ATM/ATR#DNA Damage/DNA Repair|ATM/ATR |
 CP Antibody |
|
45875-100ul |
SAB |
100ul |
EUR 302.4 |
CP Antibody |
|
45875-50ul |
SAB |
50ul |
EUR 224.4 |
CP Antibody |
|
46543 |
SAB |
100ul |
EUR 319 |
CP Antibody |
|
46543-100ul |
SAB |
100ul |
EUR 302.4 |
CP Antibody |
|
DF9369-100ul |
Affinity Biosciences |
100ul |
EUR 168 |
|
|
|
Description: WB,IHC,IF/ICC,ELISA(peptide) |
CP Antibody |
|
DF9369-200ul |
Affinity Biosciences |
200ul |
EUR 210 |
|
|
|
Description: WB,IHC,IF/ICC,ELISA(peptide) |
 CP-640186 |
|
B3585-10 |
ApexBio |
10 mg |
EUR 156 |
|
|
|
Description: isozyme-nonselective acetyl-CoA carboxylase (ACCase) inhibitor |
CP-640186 |
|
B3585-2 |
ApexBio |
2 mg |
EUR 64 |
|
|
|
Description: isozyme-nonselective acetyl-CoA carboxylase (ACCase) inhibitor |
CP-640186 |
|
B3585-5 |
ApexBio |
5 mg |
EUR 96 |
|
|
|
Description: isozyme-nonselective acetyl-CoA carboxylase (ACCase) inhibitor |
CP-640186 |
|
B3585-50 |
ApexBio |
50 mg |
EUR 616 |
|
|
|
Description: isozyme-nonselective acetyl-CoA carboxylase (ACCase) inhibitor |
 CP 80633 |
|
B7162-.5 |
ApexBio |
500ug |
EUR 40 |
|
Description: Metabolism|PDE |
CP 80633 |
|
B7162-1 |
ApexBio |
1mg |
EUR 56 |
|
Description: Metabolism|PDE |
CP 80633 |
|
B7162-10 |
ApexBio |
10 mg |
EUR 392 |
|
|
|
Description: Metabolism|PDE |
CP 80633 |
|
B7162-5 |
ApexBio |
5mg |
EUR 228 |
|
Description: Metabolism|PDE |
CP 80633 |
|
B7162-50 |
ApexBio |
50 mg |
EUR 1019 |
|
|
|
Description: PDE4 inhibitor |
 CP 154526 |
|
B7185-1 |
ApexBio |
1 mg |
EUR 66.4 |
|
|
|
Description: GPCR/G protein|CRF1 Receptors |
CP 154526 |
|
B7185-5 |
ApexBio |
5 mg |
EUR 103.2 |
|
|
|
Description: GPCR/G protein|CRF1 Receptors |
 CP 96345 |
|
B7213-10 |
ApexBio |
10 mg |
EUR 472 |
|
|
|
Description: Neuroscience|Substance P/NK1 Receptor |
CP 96345 |
|
B7213-50 |
ApexBio |
50 mg |
EUR 1482.4 |
|
|
|
Description: Neuroscience|Substance P/NK1 Receptor |
Improvement and validation of a fast package for authenticity of murine meat in meat merchandise with a species-specific PCR assay.
Adulteration of meat merchandise with murine meat poses an enormous risk to shopper well being and results in critical disruption in meals markets. Species authentication of murine meat continues to be technically difficult.
We, due to this fact, developed a species-specific PCR package consisting of murine meat DNA extraction, PCR response and figuring out techniques. We designed novel common primers focusing on extremely conserved area on mitochondrial cytochrome b gene (cyt b) from 4 murines (lab rats, lab mice, wild rat and wild mice), in addition to particular primers for meat from 4 extensively consumed animal species, cattle, sheep, duck and donkey.
Concurrently, pasmid inserted particular cyt b fragment was cloned and used as the interior positve management within the package. The package parameters of specificity, sensitivity, stability and validity had been decided utilizing mimic counterfeiting meatball.
The specificity of the DNA detection package was 100% in authentication of the 4 fraudulent meats of cattle, sheep, duck and donkey combined murine meat. The minimal detection restrict of the pattern DNA was 0.1 μg. The package, which had freeze-thawed as much as 20 instances and saved for 1 12 months, additionally was highly effective in detecting an quantity of 0.1 mg in synthetic counterfeited cattle, sheep, duck and donkey meat merchandise.
The murine-species DNA detection package proposed on this research has proved to be a easy, correct and efficient assay, and might be utilized to the identification of murine meat traces in widespread edible meat, to make sure the realisable implementation of meat product market supervision
 GPR100 Antibody |
|
E19-4888 |
EnoGene |
100μg/100μl |
EUR 225 |
|
Description: Available in various conjugation types. |
GPR100 Antibody |
|
MBS851558-01mg |
MyBiosource |
0.1mg |
EUR 305 |
GPR100 Antibody |
|
MBS851558-01mLAF405L |
MyBiosource |
0.1mL(AF405L) |
EUR 465 |
GPR100 Antibody |
|
MBS851558-01mLAF405S |
MyBiosource |
0.1mL(AF405S) |
EUR 465 |
GPR100 Antibody |
|
MBS851558-01mLAF610 |
MyBiosource |
0.1mL(AF610) |
EUR 465 |
GPR100 Antibody |
|
MBS851558-01mLAF635 |
MyBiosource |
0.1mL(AF635) |
EUR 465 |
GPR100 Antibody |
|
MBS8515590-01mg |
MyBiosource |
0.1mg |
EUR 305 |
GPR100 Antibody |
|
MBS8515590-01mLAF405L |
MyBiosource |
0.1mL(AF405L) |
EUR 465 |
GPR100 Antibody |
|
MBS8515590-01mLAF405S |
MyBiosource |
0.1mL(AF405S) |
EUR 465 |
GPR100 Antibody |
|
MBS8515590-01mLAF610 |
MyBiosource |
0.1mL(AF610) |
EUR 465 |
GPR100 Antibody |
|
MBS8515590-01mLAF635 |
MyBiosource |
0.1mL(AF635) |
EUR 465 |
GPR100 Antibody |
|
MBS9606340-01mL |
MyBiosource |
0.1mL |
EUR 260 |
GPR100 Antibody |
|
MBS9606340-02mL |
MyBiosource |
0.2mL |
EUR 305 |
GPR100 Antibody |
|
MBS9606340-5x02mL |
MyBiosource |
5x0.2mL |
EUR 1220 |
GPR100 Antibody |
|
MBS9507933-005mL |
MyBiosource |
0.05mL |
EUR 285 |
GPR100 Antibody |
|
MBS9507933-01mL |
MyBiosource |
0.1mL |
EUR 385 |
GPR100 Antibody |
|
MBS9507933-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1590 |
 GPR100 antibody |
|
MBS839751-01mg |
MyBiosource |
0.1mg |
EUR 465 |
GPR100 antibody |
|
MBS839751-5x01mg |
MyBiosource |
5x0.1mg |
EUR 1945 |
 GPR100 Polyclonal Antibody |
|
ABP54815-003ml |
Abbkine |
0.03ml |
EUR 189.6 |
|
|
|
Description: A polyclonal antibody for detection of GPR100 from Human. This GPR100 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR100 at AA rangle: 300-380 |
GPR100 Polyclonal Antibody |
|
ABP54815-01ml |
Abbkine |
0.1ml |
EUR 346.8 |
|
|
|
Description: A polyclonal antibody for detection of GPR100 from Human. This GPR100 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR100 at AA rangle: 300-380 |
GPR100 Polyclonal Antibody |
|
ABP54815-02ml |
Abbkine |
0.2ml |
EUR 496.8 |
|
|
|
Description: A polyclonal antibody for detection of GPR100 from Human. This GPR100 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR100 at AA rangle: 300-380 |
GPR100 Polyclonal Antibody |
|
BT-AP03669-100ul |
Jiaxing Korain Biotech Ltd (BT Labs) |
100ul |
Ask for price |
|
|
|
Description: GPR100 is a member of the rhodopsin family of G protein-coupled receptors (GPRs) (Fredriksson et al. 2003 [PubMed 14623098]). |
GPR100 Polyclonal Antibody |
|
BT-AP03669-20ul |
Jiaxing Korain Biotech Ltd (BT Labs) |
20ul |
Ask for price |
|
|
|
Description: GPR100 is a member of the rhodopsin family of G protein-coupled receptors (GPRs) (Fredriksson et al. 2003 [PubMed 14623098]). |
GPR100 Polyclonal Antibody |
|
BT-AP03669-50ul |
Jiaxing Korain Biotech Ltd (BT Labs) |
50ul |
Ask for price |
|
|
|
Description: GPR100 is a member of the rhodopsin family of G protein-coupled receptors (GPRs) (Fredriksson et al. 2003 [PubMed 14623098]). |
GPR100 Polyclonal Antibody |
|
JOT-AP03669-100ul |
Jotbody |
100ul |
EUR 220 |
|
|
|
Description: Human |
GPR100 Polyclonal Antibody |
|
JOT-AP03669-50ul |
Jotbody |
50ul |
EUR 144 |
|
|
|
Description: Human |
GPR100 Polyclonal Antibody |
|
E44H04840 |
EnoGene |
100ul |
EUR 255 |
|
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody |
GPR100 Polyclonal Antibody |
|
E20-71947 |
EnoGene |
100ug |
EUR 225 |
|
Description: Available in various conjugation types. |
GPR100 Polyclonal Antibody |
|
MBS2536981-002mL |
MyBiosource |
0.02mL |
EUR 140 |
GPR100 Polyclonal Antibody |
|
MBS2536981-006mL |
MyBiosource |
0.06mL |
EUR 180 |
GPR100 Polyclonal Antibody |
|
MBS2536981-012mL |
MyBiosource |
0.12mL |
EUR 260 |
GPR100 Polyclonal Antibody |
|
MBS2536981-02mL |
MyBiosource |
0.2mL |
EUR 405 |
GPR100 Polyclonal Antibody |
|
MBS9243797-01mL |
MyBiosource |
0.1mL |
EUR 415 |
GPR100 Polyclonal Antibody |
|
MBS9243797-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1841 |
GPR100 Polyclonal Antibody |
|
MBS8522928-01mg |
MyBiosource |
0.1mg |
EUR 305 |
GPR100 Polyclonal Antibody |
|
MBS8522928-01mLAF405L |
MyBiosource |
0.1mL(AF405L) |
EUR 465 |
GPR100 Polyclonal Antibody |
|
MBS8522928-01mLAF405S |
MyBiosource |
0.1mL(AF405S) |
EUR 465 |
GPR100 Polyclonal Antibody |
|
MBS8522928-01mLAF610 |
MyBiosource |
0.1mL(AF610) |
EUR 465 |
GPR100 Polyclonal Antibody |
|
MBS8522928-01mLAF635 |
MyBiosource |
0.1mL(AF635) |
EUR 465 |
GPR100 Polyclonal Antibody |
|
UB-GEN-13073 |
UpingBio |
100 ul |
EUR 200 |
 GPR100 Rabbit Polyclonal Antibody |
|
ES5814-100ul |
ELK Biotech |
100ul |
EUR 124 |
|
|
|
Description: WB, IF, ELISA |
GPR100 Rabbit Polyclonal Antibody |
|
ES5814-50ul |
ELK Biotech |
50ul |
EUR 74 |
|
|
|
Description: WB, IF, ELISA |
 Antibody) G-Protein Coupled Receptor 100 (GPR100) Antibody |
|
abx015249-100g |
Abbexa |
100 µg |
EUR 43.75 |
G-Protein Coupled Receptor 100 (GPR100) Antibody |
|
20-abx015249 |
Abbexa |
-
Ask for price
-
Ask for price
-
Ask for price
-
Ask for price
|
- 10 ug
- 100 ug
- 200 ug
- 300 µg
|
|
|
 GPR100 Blocking Peptide |
|
DF4888-BP |
Affbiotech |
1mg |
EUR 234 |
GPR100 Blocking Peptide |
|
MBS9625057-1mg |
MyBiosource |
1mg |
EUR 380 |
GPR100 Blocking Peptide |
|
MBS9625057-5x1mg |
MyBiosource |
5x1mg |
EUR 1650 |
 Rabbit Polyclonal to Human RXFP4 / GPR100 |
|
MBS242737-005mg |
MyBiosource |
0.05mg |
EUR 615 |
Rabbit Polyclonal to Human RXFP4 / GPR100 |
|
MBS242737-5x005mg |
MyBiosource |
5x0.05mg |
EUR 2605 |
Rabbit Polyclonal to Human RXFP4 / GPR100 |
|
MBS246803-005mg |
MyBiosource |
0.05mg |
EUR 615 |
Rabbit Polyclonal to Human RXFP4 / GPR100 |
|
MBS246803-5x005mg |
MyBiosource |
5x0.05mg |
EUR 2605 |
 Anti- GPR162 Antibody Antibody |
|
GWB-AAD3BE |
GenWay Biotech |
0.05 ml |
Ask for price |
Anti- GPR162 Antibody Antibody |
|
GWB-C5010A |
GenWay Biotech |
0.05 ml |
Ask for price |
Anti- GPR162 Antibody Antibody |
|
GWB-39B7E0 |
GenWay Biotech |
0.05 ml |
Ask for price |
|
|
 Anti-GPR100 Antibody |
|
MBS8308011-005mL |
MyBiosource |
0.05mL |
EUR 210 |
Anti-GPR100 Antibody |
|
MBS8308011-01mL |
MyBiosource |
0.1mL |
EUR 255 |
Anti-GPR100 Antibody |
|
MBS8308011-02mL |
MyBiosource |
0.2mL |
EUR 335 |
Anti-GPR100 Antibody |
|
MBS8308011-5x02mL |
MyBiosource |
5x0.2mL |
EUR 1420 |
 Anti-GPR100 antibody |
|
STJ93312 |
St John's Laboratory |
200 µl |
EUR 236.4 |
|
Description: Rabbit polyclonal to GPR100. |
 anti- GPR105 antibody |
|
FNab03588 |
FN Test |
100µg |
EUR 658.5 |
|
|
|
Description: Antibody raised against GPR105 |
 anti- GPR107 antibody |
|
FNab03589 |
FN Test |
100µg |
EUR 702 |
|
|
|
Description: Antibody raised against GPR107 |
 anti- GPR108 antibody |
|
FNab03590 |
FN Test |
100µg |
EUR 658.5 |
|
|
|
Description: Antibody raised against GPR108 |
 anti- GPR116 antibody |
|
FNab03591 |
FN Test |
100µg |
EUR 658.5 |
|
|
|
Description: Antibody raised against GPR116 |
 anti- GPR132 antibody |
|
FNab03593 |
FN Test |
100µg |
EUR 606.3 |
|
|
|
Description: Antibody raised against GPR132 |
 anti- GPR137 antibody |
|
FNab03594 |
FN Test |
100µg |
EUR 606.3 |
|
|
|
Description: Antibody raised against GPR137 |
 anti- GPR142 antibody |
|
FNab03597 |
FN Test |
100µg |
EUR 702 |
|
|
|
Description: Antibody raised against GPR142 |
 anti- GPR161 antibody |
|
FNab03598 |
FN Test |
100µg |
EUR 606.3 |
|
|
|
Description: Antibody raised against GPR161 |
 anti- GPR162 antibody |
|
FNab03599 |
FN Test |
100µg |
EUR 702 |
|
|
|
Description: Antibody raised against GPR162 |
 anti- GPR177 antibody |
|
FNab03601 |
FN Test |
100µg |
EUR 658.5 |
|
|
|
Description: Antibody raised against GPR177 |
 anti- GPR137B antibody |
|
FNab03595 |
FN Test |
100µg |
EUR 702 |
|
|
|
Description: Antibody raised against GPR137B |
 anti- GPR17 antibody |
|
FNab03600 |
FN Test |
100µg |
EUR 658.5 |
|
|
|
Description: Antibody raised against GPR17 |
 anti- GPR22 antibody |
|
FNab03603 |
FN Test |
100µg |
EUR 606.3 |
|
|
|
Description: Antibody raised against GPR22 |
 anti- GPR35 antibody |
|
FNab03605 |
FN Test |
100µg |
EUR 702 |
|
|
|
Description: Antibody raised against GPR35 |
 anti- GPR50 antibody |
|
FNab03607 |
FN Test |
100µg |
EUR 658.5 |
|
|
|
Description: Antibody raised against GPR50 |
anti- GPR50 antibody |
|
FNab03608 |
FN Test |
100µg |
EUR 658.5 |
|
|
|
Description: Antibody raised against GPR50 |
 anti- GPR81 antibody |
|
FNab03610 |
FN Test |
100µg |
EUR 702 |
|
|
|
Description: Antibody raised against GPR81 |
 anti- GPR83 antibody |
|
FNab03611 |
FN Test |
100µg |
EUR 702 |
|
|
|
Description: Antibody raised against GPR83 |
 Anti-G2A GPR132 Antibody |
|
A07182 |
BosterBio |
100ul |
EUR 399 |
|
|
|
Description: Boster Bio Anti-G2A GPR132 Antibody catalog # A07182. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse. |
 anti- GPR183/EBI2 antibody |
|
FNab03602 |
FN Test |
100µg |
EUR 658.5 |
|
|
|
Description: Antibody raised against GPR183/EBI2 |
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