PCR for the detection of vector copy quantity in scientific CAR/TCR T cell

Utility of droplet digital PCR for the detection of vector copy quantity in scientific CAR/TCR T cell merchandise.

Genetically engineered T cells have turn into an essential remedy for B-cell malignancies. Measuring the effectivity of vector integration into the T cell genome is essential for assessing the efficiency and security of those most cancers immunotherapies.A digital droplet polymerase chain response (ddPCR) assay was developed and evaluated for assessing the common variety of lenti- and retroviral vectors built-in into Chimeric Antigen Receptor (CAR) and T Cell Receptor (TCR)-engineered T cells.

 

  • The ddPCR assay constantly measured the focus of an empty vector in answer and the common variety of CAR and TCR vectors built-in into T cell populations.
  • There was a linear relationship between the common vector copy quantity per cell measured by ddPCR and the proportion of cells transduced as measured by stream cytometry.
  • Related vector copy quantity measurements had been obtained by completely different employees utilizing the ddPCR assay, highlighting the assays reproducibility amongst technicians. Evaluation of contemporary and cryopreserved CAR T and TCR engineered T cells yielded comparable outcomes.
  • ddPCR is a sturdy software for correct quantitation of common vector copy quantity in CAR and TCR engineered T cells. The assay can be relevant to different kinds of genetically engineered cells together with Pure Killer cells and hematopoietic stem cells.

Improvement of a PCR-based lateral stream strip assay for the straightforward, fast, and correct detection of pork in meat and meat merchandise.

In recent times, adulteration of meat and meat merchandise has turn into a significant meals security problem. PCR and real-time PCR applied sciences are mainstream strategies used to determine animal-derived elements.

Nonetheless, these applied sciences rely extremely on pricey gear {and professional} technicians; they’re due to this fact tough to make use of in resource-limited settings. On this research, a novel, extremely delicate molecular assay, Pig-PCR-Strip (Pig particular polymerase chain reaction-Lateral stream strip), was developed for fast detection of pig and swine-derived elements.

The assay relies on PCR amplification, hybridization of the PCR product to the probe, adopted by detection utilizing a strip format. Utilizing this format, the PCR product might be detected by the bare eye inside three min, and gives a foundation for the migration of species-specific detection to a point-of-care (POC) microfluidic format.

The Pig-PCR-Strip can detect pork elements at a focus of 0.01% in adulterated meat, and the restrict of detection is as much as 10 fg of goal DNA. The assay was particular to pork and didn’t cross-react with different non-target species. It additionally can be utilized for industrial samples and sophisticated meals samples.

It’s a promising new software for detection of pig-derived meat and might be quickly modified for figuring out different species. It might be extensively used for fixing issues associated to meat high quality assurance, species authentication, and traceability.

genpcr

genpcr

CP-640186

9422-25 each
EUR 666

CP-640186

9422-5 each
EUR 222

CP-466722

A8625-10 10 mg
EUR 136
Description: ATM inhibitor,potent and reversible

CP-466722

A8625-5 5 mg
EUR 88
Description: ATM inhibitor,potent and reversible

CP-466722

A8625-50 50 mg
EUR 374
Description: ATM inhibitor,potent and reversible

CP-91149

A8403-10 10 mg
EUR 105
Description: Selective inhibitor of glycogen phosphorylase

CP-91149

A8403-25 25 mg
EUR 210
Description: Selective inhibitor of glycogen phosphorylase

CP-91149

A8403-5 5 mg
EUR 63
Description: Selective inhibitor of glycogen phosphorylase

Coelenterazine CP

10112 50uG
EUR 77
Description: N/A

Coelenterazine CP

10112-1 1MG
EUR 77
Description: N/A

Coelenterazine CP

10112-1-1 EA
EUR 831

Coelenterazine CP

10112-2 250uG
EUR 257
Description: N/A

Coelenterazine CP

10112-2-1 EA
EUR 257

CP-690550

1622-25 each
EUR 738

CP-690550

1622-5 each
EUR 255.6

CP-609754

206096 10.0mg
EUR 450

CP-547632

200810 5.0mg
EUR 300

CP-724714

200820 5.0mg
EUR 250

CP-346086

471083 5.0mg
EUR 270

CP-335963

471085 1.0g
EUR 170

CP-101537

471091 10.0mg
EUR 90

CP Antibody

46543 100ul
EUR 319

CP Antibody

46543-100ul 100ul
EUR 302.4

CP Antibody

45875-100ul 100ul
EUR 302.4

CP Antibody

45875-50ul 50ul
EUR 224.4

CP 135807

413313 5.0mg
EUR 275

CP Antibody

E046543 100μg/100μl
EUR 255
Description: Available in various conjugation types.

CP Antibody

DF9369 200ul
EUR 420

CP Antibody

DF9369-100ul 100ul
EUR 280

CP Antibody

DF9369-200ul 200ul
EUR 350

CP Antibody

E19-9369-1 50ug/50ul
EUR 145
Description: Available in various conjugation types.

CP Antibody

E19-9369-2 100ug/100ul
EUR 225
Description: Available in various conjugation types.

CP-466722

B1722-1 each
EUR 183.6

CP-466722

B1722-5 each
EUR 418.8

CP-547632

B2056-500 each
EUR 183.6

CP 135807

B7497-10 10 mg
EUR 270
Description: 5-HT1D receptor agonist

CP 135807

B7497-50 50 mg
EUR 1096
Description: 5-HT1D receptor agonist

CP 775146

B7609-10 10 mg
EUR 308
Description: PPARα agonist

CP 775146

B7609-50 50 mg
EUR 1234
Description: PPARα agonist

CP 80633

B7162-10 10 mg
EUR 258
Description: PDE4 inhibitor

CP 80633

B7162-50 50 mg
EUR 1019
Description: PDE4 inhibitor

CP 154526

B7185-1 1 mg
EUR 48
Description: CRF1 receptor antagonist

CP 154526

B7185-5 5 mg
EUR 78
Description: CRF1 receptor antagonist

CP 96345

B7213-10 10 mg
EUR 285
Description: NK1 receptor antagonist

CP 96345

B7213-50 50 mg
EUR 1163
Description: NK1 receptor antagonist

CP-424174

B2531-1 each
EUR 157.2

CP-424174

B2531-5 each
EUR 405.6

CP-544326

B1121-25 each
EUR 666

CP-544326

B1121-5 each
EUR 222

CP-724714

B1333-25 each
EUR 940.8

CP-724714

B1333-5 each
EUR 314.4

CP-673451

B1337-1 each
EUR 288

CP-673451

B1337-5 each
EUR 810

CP-673451

B2173-10 10 mg
EUR 180
Description: PDGFRα/β inhibitor,potent and selective

CP-673451

B2173-200 200 mg
EUR 1672
Description: PDGFRα/β inhibitor,potent and selective

CP-673451

B2173-5 5 mg
EUR 95
Description: PDGFRα/β inhibitor,potent and selective

CP-673451

B2173-5.1 10 mM (in 1mL DMSO)
EUR 128
Description: PDGFRα/β inhibitor,potent and selective

CP-673451

B2173-50 50 mg
EUR 543
Description: PDGFRα/β inhibitor,potent and selective

CP 316819

B5448-10 10 mg
EUR 298
Description: Selective glycogen phosphorylase inhibitor

CP 316819

B5448-50 50 mg
EUR 1185
Description: Selective glycogen phosphorylase inhibitor

CP 20961

B5474-10 10 mg
EUR 226
Description: non-immunogenic adjuvant

CP 20961

B5474-50 50 mg
EUR 952
Description: non-immunogenic adjuvant

CP-640186

B3585-10 10 mg
EUR 156
Description: isozyme-nonselective acetyl-CoA carboxylase (ACCase) inhibitor

CP-640186

B3585-2 2 mg
EUR 64
Description: isozyme-nonselective acetyl-CoA carboxylase (ACCase) inhibitor

CP-640186

B3585-5 5 mg
EUR 96
Description: isozyme-nonselective acetyl-CoA carboxylase (ACCase) inhibitor

CP-640186

B3585-50 50 mg
EUR 616
Description: isozyme-nonselective acetyl-CoA carboxylase (ACCase) inhibitor

CP-10447

C781200 10mg
EUR 201
Description: 843-93-6

CP 96345

C781210 250mg
EUR 9200
Description: 132746-60-2

(±)-CP 55940

C781225 0.5mg
EUR 1518

CP-141938

C781290 100mg
EUR 12800
Description: 182822-62-4

CP-316819

C781300 10mg
EUR 356

Improvement and validation of a fast package for authenticity of murine meat in meat merchandise with a species-specific PCR assay.

Adulteration of meat merchandise with murine meat poses an enormous risk to shopper well being and results in critical disruption in meals markets. Species authentication of murine meat continues to be technically difficult.

 

We, due to this fact, developed a species-specific PCR package consisting of murine meat DNA extraction, PCR response and figuring out techniques. We designed novel common primers focusing on extremely conserved area on mitochondrial cytochrome b gene (cyt b) from 4 murines (lab rats, lab mice, wild rat and wild mice), in addition to particular primers for meat from 4 extensively consumed animal species, cattle, sheep, duck and donkey.

 

Concurrently, pasmid inserted particular cyt b fragment was cloned and used as the interior positve management within the package. The package parameters of specificity, sensitivity, stability and validity had been decided utilizing mimic counterfeiting meatball.

 

The specificity of the DNA detection package was 100% in authentication of the 4 fraudulent meats of cattle, sheep, duck and donkey combined murine meat. The minimal detection restrict of the pattern DNA was 0.1 μg. The package, which had freeze-thawed as much as 20 instances and saved for 1 12 months, additionally was highly effective in detecting an quantity of 0.1 mg in synthetic counterfeited cattle, sheep, duck and donkey meat merchandise.

 

The murine-species DNA detection package proposed on this research has proved to be a easy, correct and efficient assay, and might be utilized to the identification of murine meat traces in widespread edible meat, to make sure the realisable implementation of meat product market supervision

 

GPR100 Antibody

MBS851558-01mLAF405S 0.1mL(AF405S)
EUR 465

GPR100 Antibody

MBS851558-01mLAF610 0.1mL(AF610)
EUR 465

GPR100 Antibody

MBS851558-01mLAF635 0.1mL(AF635)
EUR 465

GPR100 Antibody

MBS8515590-01mg 0.1mg
EUR 305

GPR100 Antibody

MBS8515590-01mLAF405L 0.1mL(AF405L)
EUR 465

GPR100 Antibody

MBS8515590-01mLAF405S 0.1mL(AF405S)
EUR 465

GPR100 Antibody

MBS8515590-01mLAF610 0.1mL(AF610)
EUR 465

GPR100 Antibody

MBS8515590-01mLAF635 0.1mL(AF635)
EUR 465

GPR100 antibody

MBS839751-01mg 0.1mg
EUR 465

GPR100 antibody

MBS839751-5x01mg 5x0.1mg
EUR 1945

GPR100 Antibody

MBS9606340-01mL 0.1mL
EUR 260

GPR100 Antibody

MBS9606340-02mL 0.2mL
EUR 305

GPR100 Antibody

MBS9606340-5x02mL 5x0.2mL
EUR 1220

GPR100 Antibody

MBS9507933-005mL 0.05mL
EUR 285

GPR100 Antibody

MBS9507933-01mL 0.1mL
EUR 385

GPR100 Antibody

MBS9507933-5x01mL 5x0.1mL
EUR 1590

GPR100 Antibody

G117-100ul 100μl
EUR 217
Description: GPR100 Rabbit Polyclonal Antibody

GPR100 Antibody

G117-50ul 50μl
EUR 143.5
Description: GPR100 Rabbit Polyclonal Antibody

GPR100 Polyclonal Antibody

ABP54815-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of GPR100 from Human. This GPR100 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR100 at AA rangle: 300-380

GPR100 Polyclonal Antibody

ABP54815-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of GPR100 from Human. This GPR100 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR100 at AA rangle: 300-380

GPR100 Polyclonal Antibody

ABP54815-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of GPR100 from Human. This GPR100 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR100 at AA rangle: 300-380

GPR100 Polyclonal Antibody

E20-71947 100ug
EUR 225
Description: Available in various conjugation types.

GPR100 Polyclonal Antibody

BT-AP03669-100ul 100ul Ask for price
Description: GPR100 is a member of the rhodopsin family of G protein-coupled receptors (GPRs) (Fredriksson et al. 2003 [PubMed 14623098]).

GPR100 Polyclonal Antibody

BT-AP03669-20ul 20ul Ask for price
Description: GPR100 is a member of the rhodopsin family of G protein-coupled receptors (GPRs) (Fredriksson et al. 2003 [PubMed 14623098]).

GPR100 Polyclonal Antibody

BT-AP03669-50ul 50ul Ask for price
Description: GPR100 is a member of the rhodopsin family of G protein-coupled receptors (GPRs) (Fredriksson et al. 2003 [PubMed 14623098]).

GPR100 Polyclonal Antibody

E44H04840 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

GPR100 Polyclonal Antibody

MBS9243797-01mL 0.1mL
EUR 415

GPR100 Polyclonal Antibody

MBS9243797-5x01mL 5x0.1mL
EUR 1841

GPR100 Polyclonal Antibody

MBS8522928-01mg 0.1mg
EUR 305

GPR100 Polyclonal Antibody

MBS8522928-01mLAF405L 0.1mL(AF405L)
EUR 465

GPR100 Polyclonal Antibody

MBS8522928-01mLAF405S 0.1mL(AF405S)
EUR 465

GPR100 Polyclonal Antibody

MBS8522928-01mLAF610 0.1mL(AF610)
EUR 465

GPR100 Polyclonal Antibody

MBS8522928-01mLAF635 0.1mL(AF635)
EUR 465

GPR100 Polyclonal Antibody

MBS2536981-002mL 0.02mL
EUR 140

GPR100 Polyclonal Antibody

MBS2536981-006mL 0.06mL
EUR 180

GPR100 Polyclonal Antibody

MBS2536981-012mL 0.12mL
EUR 260

GPR100 Polyclonal Antibody

MBS2536981-02mL 0.2mL
EUR 405

GPR100 Rabbit Polyclonal Antibody

ES5814-100ul 100ul
EUR 124
Description: A Rabbit Polyclonal antibody against GPR100 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA

GPR100 Rabbit Polyclonal Antibody

ES5814-50ul 50ul
EUR 74
Description: A Rabbit Polyclonal antibody against GPR100 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA

G-Protein Coupled Receptor 100 (GPR100) Antibody

20-abx015249
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

G-Protein Coupled Receptor 100 (GPR100) Antibody

abx015249-100g 100 µg
EUR 43.75

GPR100 Blocking Peptide

DF4888-BP 1mg
EUR 234

GPR100 Blocking Peptide

MBS9625057-1mg 1mg
EUR 380

GPR100 Blocking Peptide

MBS9625057-5x1mg 5x1mg
EUR 1650

Rabbit Polyclonal to Human RXFP4 / GPR100

MBS242737-005mg 0.05mg
EUR 615

Rabbit Polyclonal to Human RXFP4 / GPR100

MBS242737-5x005mg 5x0.05mg
EUR 2605

Rabbit Polyclonal to Human RXFP4 / GPR100

MBS246803-005mg 0.05mg
EUR 615

Rabbit Polyclonal to Human RXFP4 / GPR100

MBS246803-5x005mg 5x0.05mg
EUR 2605

Anti- GPR162 Antibody Antibody

GWB-39B7E0 0.05 ml Ask for price

Anti- GPR162 Antibody Antibody

GWB-AAD3BE 0.05 ml Ask for price

Anti- GPR162 Antibody Antibody

GWB-C5010A 0.05 ml Ask for price

Anti-GPR100 Antibody

A09371 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for GPR100 Antibody (RXFP4) detection.tested for WB in Human.

Anti-GPR100 antibody

STJ93312 200 µl
EUR 236.4
Description: Rabbit polyclonal to GPR100.

Anti-GPR100 Antibody

MBS8308011-005mL 0.05mL
EUR 210

Anti-GPR100 Antibody

MBS8308011-01mL 0.1mL
EUR 255

Anti-GPR100 Antibody

MBS8308011-02mL 0.2mL
EUR 335

Anti-GPR100 Antibody

MBS8308011-5x02mL 5x0.2mL
EUR 1420

anti- GPR105 antibody

FNab03588 100µg
EUR 658.5
Description: Antibody raised against GPR105

anti- GPR107 antibody

FNab03589 100µg
EUR 702
Description: Antibody raised against GPR107

anti- GPR108 antibody

FNab03590 100µg
EUR 658.5
Description: Antibody raised against GPR108

anti- GPR116 antibody

FNab03591 100µg
EUR 658.5
Description: Antibody raised against GPR116

anti- GPR132 antibody

FNab03593 100µg
EUR 606.3
Description: Antibody raised against GPR132

anti- GPR137 antibody

FNab03594 100µg
EUR 606.3
Description: Antibody raised against GPR137

anti- GPR142 antibody

FNab03597 100µg
EUR 702
Description: Antibody raised against GPR142

anti- GPR161 antibody

FNab03598 100µg
EUR 606.3
Description: Antibody raised against GPR161

anti- GPR162 antibody

FNab03599 100µg
EUR 702
Description: Antibody raised against GPR162

anti- GPR177 antibody

FNab03601 100µg
EUR 658.5
Description: Antibody raised against GPR177

anti- GPR137B antibody

FNab03595 100µg
EUR 702
Description: Antibody raised against GPR137B

anti- GPR17 antibody

FNab03600 100µg
EUR 658.5
Description: Antibody raised against GPR17

anti- GPR22 antibody

FNab03603 100µg
EUR 606.3
Description: Antibody raised against GPR22

anti- GPR35 antibody

FNab03605 100µg
EUR 702
Description: Antibody raised against GPR35

anti- GPR50 antibody

FNab03607 100µg
EUR 658.5
Description: Antibody raised against GPR50

anti- GPR50 antibody

FNab03608 100µg
EUR 658.5
Description: Antibody raised against GPR50

anti- GPR81 antibody

FNab03610 100µg
EUR 702
Description: Antibody raised against GPR81

anti- GPR83 antibody

FNab03611 100µg
EUR 702
Description: Antibody raised against GPR83

anti- GPR183/EBI2 antibody

FNab03602 100µg
EUR 658.5
Description: Antibody raised against GPR183/EBI2

Anti-GP183 GPR183 Antibody

A02573 100ul
EUR 380
Description: Boster Bio Anti-GP183 GPR183 Antibody catalog # A02573. Tested in ELISA, WB applications. This antibody reacts with Human.

Anti-GP179 GPR179 Antibody

A10399-1 100ul
EUR 380
Description: Boster Bio Anti-GP179 GPR179 Antibody catalog # A10399-1. Tested in IHC applications. This antibody reacts with Human.

Anti-OR51E1/Gpr164 Antibody

A30874 100ul
EUR 476.4
Description: Rabbit Polyclonal OR51E1/Gpr164 Antibody. Validated in IF, WB and tested in Human.

Anti-Protein GPR107 GPR107 Antibody

A10357-1 100ul
EUR 380
Description: Boster Bio Anti-Protein GPR107 GPR107 Antibody catalog # A10357-1. Tested in ELISA, IF, IHC-P, WB applications. This antibody reacts with Human.

anti- GPR3 antibody

FNab03604 100µg
EUR 702
Description: Antibody raised against GPR3
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